Comparability of corneal thickness and opacity depth assessed by OCT and UBM

Garcia, Patricia Novita; Chamon, Wallace; Allemann, Norma

doi:10.1007/s00417-021-05161-9

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…From the perspective of detection depth, more and more clinical diagnostic methods have shown considerable advantages. Garcia et al [125] used Optovue OCT, Visaante OCT and ultrasonic thickness gauge to diagnose opaque cornea, and finally measured depths of 534.03 μm, 523.72 μm, and 529.84 μm, which were consistent with the study of MNA penetration depth. The study used a cornea that is very different from the skin tissue, but it provides an alternative method in terms of the detectable depth.…”

Section: Strategies For Appropriate Imagingmentioning

confidence: 54%

Bioimaging of Dissolvable Microneedle Arrays: Challenges and Opportunities

Yanni

Tao

et al. 2022

Research

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The emergence of microneedle arrays (MNAs) as a novel, simple, and minimally invasive administration approach largely addresses the challenges of traditional drug delivery. In particular, the dissolvable MNAs act as a promising, multifarious, and well-controlled platform for micro-nanotransport in medical research and cosmetic formulation applications. The effective delivery mostly depends on the behavior of the MNAs penetrated into the body, and accurate assessment is urgently needed. Advanced imaging technologies offer high sensitivity and resolution visualization of cross-scale, multidimensional, and multiparameter information, which can be used as an important aid for the evaluation and development of new MNAs. The combination of MNA technology and imaging can generate considerable new knowledge in a cost-effective manner with regards to the pharmacokinetics and bioavailability of active substances for the treatment of various diseases. In addition, noninvasive imaging techniques allow rapid, receptive assessment of transdermal penetration and drug deposition in various tissues, which could greatly facilitate the translation of experimental MNAs into clinical application. Relying on the recent promising development of bioimaging, this review is aimed at summarizing the current status, challenges, and future perspective on in vivo assessment of MNA drug delivery by various imaging technologies.

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Section: Strategies For Appropriate Imagingmentioning

confidence: 54%

Bioimaging of Dissolvable Microneedle Arrays: Challenges and Opportunities

Yanni

Tao

et al. 2022

Research

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“…Although SMILE is considered a safe, effective, and predictable method in the treatment of myopia and myopic astigmatism, it is important to understand the influence of cap morphology on refractive results. AS-OCT is very useful in analyzing the morphology of the cornea, since it is a contact-free method which is easy to use and allows for the view of a large surface of the cornea at different depths, as well as for the direct measurement of the cap thickness in different meridians [19][20][21][22][23][24].…”

Section: Discussionmentioning

confidence: 99%

Assessment of Changes in Cap and Residual Stromal Thickness Values during a 6-Month Observation after Refractive Lenticule Extraction Small Incision Lenticule Extraction

Janiszewska-Bil,

Czarnota-Nowakowska,

Kuciel-Polczak

et al. 2024

JCM

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Background: In this study, the changes in corneal cap and residual stromal thickness (RST) values during a 180-day observation period after refractive lenticule extraction small incision lenticule extraction (ReLEx SMILE) were assessed. Methods: Fifty patients underwent ReLEx SMILE using the VisuMax 500 femtosecond laser, with corneal imaging conducted pre and post procedure via anterior segment optical coherence tomography (AS-OCT). Cap thickness in the center and 1.5 mm from the center in four meridians was measured at various intervals. Results: The results showed a significant decrease in cap thickness 180 days post procedure compared to earlier intervals (p < 0.05). Similarly, RST decreased gradually and significantly post procedure (p < 0.05). Notably, changes in cap thickness within the central 1.5 mm area were more dynamic than RST changes during the 6-month observation period following SMILE. Conclusions: The corneal cap thickness measured with swept-source AS-OCT within the central 1.5 mm area underwent more dynamic changes than the residual stromal thickness during the 6-month observation following SMILE.

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“…A macroscopic evaluation was carried out on each animal, and slit lamp and OCT images were obtained from the right and left eyes after 3 and 12 months of the implant. Corneal opacity was graded according to the classification established by García and cols ( Garcia et al, 2021 ). as mild (grade I), moderate (grade II) and dense (grade III).…”

Section: Methodsmentioning

confidence: 99%

Development of stromal differentiation patterns in heterotypical models of artificial corneas generated by tissue engineering

Blanco-Elices

Morales-Álvarez

Chato‐Astrain

et al. 2023

Front. Bioeng. Biotechnol.

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Purpose: We carried out a histological characterization analysis of the stromal layer of human heterotypic cornea substitutes generated with extra-corneal cells to determine their putative usefulness in tissue engineering.Methods: Human bioartificial corneas were generated using nanostructured fibrin-agarose biomaterials with corneal stromal cells immersed within. To generate heterotypical corneas, umbilical cord Wharton’s jelly stem cells (HWJSC) were cultured on the surface of the stromal substitutes to obtain an epithelial-like layer. These bioartificial corneas were compared with control native human corneas and with orthotypical corneas generated with human corneal epithelial cells on top of the stromal substitute. Both the corneal stroma and the basement membrane were analyzed using histological, histochemical and immunohistochemical methods in samples kept in culture and grafted in vivo for 12 months in the rabbit cornea.Results: Our results showed that the stroma of the bioartificial corneas kept ex vivo showed very low levels of fibrillar and non-fibrillar components of the tissue extracellular matrix. However, in vivo implantation resulted in a significant increase of the contents of collagen, proteoglycans, decorin, keratocan and lumican in the corneal stroma, showing higher levels of maturation and spatial organization of these components. Heterotypical corneas grafted in vivo for 12 months showed significantly higher contents of collagen fibers, proteoglycans and keratocan. When the basement membrane was analyzed, we found that all corneas grafted in vivo showed intense PAS signal and higher contents of nidogen-1, although the levels found in human native corneas was not reached, and a rudimentary basement membrane was observed using transmission electron microscopy. At the epithelial level, HWJSC used to generate an epithelial-like layer in ex vivo corneas were mostly negative for p63, whereas orthotypical corneas and heterotypical corneas grafted in vivo were positive.Conclusion: These results support the possibility of generating bioengineered artificial corneas using non-corneal HWJSC. Although heterotypical corneas were not completely biomimetic to the native human corneas, especially ex vivo, in vivo grafted corneas demonstrated to be highly biocompatible, and the animal cornea became properly differentiated at the stroma and basement membrane compartments. These findings open the door to the future clinical use of these bioartificial corneas.

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Comparability of corneal thickness and opacity depth assessed by OCT and UBM

Cited by 6 publications

References 25 publications

Bioimaging of Dissolvable Microneedle Arrays: Challenges and Opportunities

Bioimaging of Dissolvable Microneedle Arrays: Challenges and Opportunities

Assessment of Changes in Cap and Residual Stromal Thickness Values during a 6-Month Observation after Refractive Lenticule Extraction Small Incision Lenticule Extraction

Development of stromal differentiation patterns in heterotypical models of artificial corneas generated by tissue engineering

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