Compactin — A new orobol bioside fromGenista compacta

Ozimina, I. I.; Bandyukova, V. A.

doi:10.1007/bf00579140

Cited by 2 publications

(2 citation statements)

References 3 publications

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“…Although no compound corresponding to this molecular weight was found in the output from the DNP, based on the molecular weight and the retention factor ( R f ), this compound was proposed to be a tetrahydroxyisoflavone. Only one tetrahydroxyisoflavone has been reported from plant of the Genista genus, the compactin isolated from Genista compacta [16]. As compactin is glycosylated, the product found in spot A2 could be the corresponding aglycone, i.e.…”

Section: Resultsmentioning

confidence: 99%

Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS

et al. 2014

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Genista species are sources of antioxidant phenolic compounds such as O-and C-glycosylflavonoids and isoflavonoids. A combination of a DPPH scavenging assay with HPTLC-MS, a fast and efficient method for identification of bioactive compounds, has been applied for evaluation of the radical scavenging activity of metabolites from Genista saharae Coss. & Dur. Different organs collected at various periods have been compared. Identification of antioxidant compounds was obtained by elution of the major DPPH-inhibition zones. The resulting HPTLC-MS analysis under moderately polar conditions, coupled to the DPPH results led to the putative identification of two antioxidant isoflavone aglycones: 3',4',5,7-tetrahydroxyisoflavone (1) and ficuisoflavone (3), whereas polar migration conditions led to the identification of the glycosides 5-methoxy-4',7-trihydroxy-8-glucopyranosylisoflavone (4) and 4',5-dihydroxy-7-methoxyisoflavone-4'-O-β-D-glucopyranoside (5). Evaluation of percentage of inhibition of DPPH radical by the purified isoflavone 4 from the root extract showed that it affords a moderate contribution to the total radical scavenging activity of the extract.

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Section: Resultsmentioning

confidence: 99%

Rapid Identification of Antioxidant Compounds of Genista saharae Coss. & Dur. by Combination of DPPH Scavenging Assay and HPTLC-MS

et al. 2014

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“…31 Again a signicant decrease in retention time under UPLC-MS conditions relative to biochanin A would support additional hydroxylation in the B-ring; in addition, an increased retention time of 2.93 min relative to orobol (Table 2) ts well with the equivalent avonoid pairing of luteolin and diosmetin where methylation of the 4 0 -OH leads to an increased retention time of 2.37 min (Table 1). However, alternative structures for Gt 4 include 3 0 -O-methylorobol, the isoavonoid equivalent of chrysoeriol (4), or 7-O-methylorobol possibly formed by alkylation of a 7-O-glycosidic species 32 under the acidic extraction conditions, as recently reported in a study of the constituents of sawwort (Serratula tinctoria L.). 33 However, it is clear that it is not the 5-O-methylated derivative as this, by analogy with isoprunetin, would be expected to elute much earlier in the UPLC chromatogram.…”

Section: Uplc-ms Studymentioning

confidence: 95%