Compact and fully automated system for monitoring photodynamic therapy, based on two LEDs and a single CCD

Kleshnin, Mikhail; Fiks, I. I.; Плеханов, В. И.; Gamayunov, S. V.; Turchin, Ilya V.

doi:10.1088/1612-2011/12/11/115602

Cited by 21 publications

(12 citation statements)

References 25 publications

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“…In the phantom and in vivo experiments, a custom-built fluorescence imaging device [ 20 , 29 ] was employed for the registration of the fluorescence from chlorin-based PSs with the absorption and emission spectra as in Figure 1 . The setup featured two LED sources at the wavelengths of 405 ± 10 nm (blue) and 660 ± 10 nm (red) synchronized with the CCD camera.…”

Section: Methodsmentioning

confidence: 99%

Dual-Wavelength Fluorescence Monitoring of Photodynamic Therapy: From Analytical Models to Clinical Studies

Kirillin

Khilov

Kurakina

et al. 2021

Cancers

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Fluorescence imaging modalities are currently a routine tool for the assessment of marker distribution within biological tissues, including monitoring of fluorescent photosensitizers (PSs) in photodynamic therapy (PDT). Conventional fluorescence imaging techniques provide en-face two-dimensional images, while depth-resolved techniques require complicated tomographic modalities. In this paper, we report on a cost-effective approach for the estimation of fluorophore localization depth based on dual-wavelength probing. Owing to significant difference in optical properties of superficial biotissues for red and blue ranges of optical spectra, simultaneous detection of fluorescence excited at different wavelengths provides complementary information from different measurement volumes. Here, we report analytical and numerical models of the dual-wavelength fluorescence imaging of PS-containing biotissues considering topical and intravenous PS administration, and demonstrate the feasibility of this approach for evaluation of the PS localization depth based on the fluorescence signal ratio. The results of analytical and numerical simulations, as well as phantom experiments, were translated to the in vivo imaging to interpret experimental observations in animal experiments, human volunteers, and clinical studies. The proposed approach allowed us to estimate typical accumulation depths of PS localization which are consistent with the morphologically expected values for both topical PS administration and intravenous injection.

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Section: Methodsmentioning

confidence: 99%

Dual-Wavelength Fluorescence Monitoring of Photodynamic Therapy: From Analytical Models to Clinical Studies

Kirillin

Khilov

Kurakina

et al. 2021

Cancers

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“…Monitoring of PS accumulation in the target area and its photobleaching in the course of the PDT procedure was performed by an FI device (IAP RAS, Russia) with probing wavelengths of 405 and 660 nm corresponding to the absorption bands of chlorin e6, and fluorescence detection in the range of 702 to 842 nm. 33,39 The device provides two-dimensional fluorescence images of the target area at both excitation wavelengths. This approach allows to indirectly estimate the PS penetration depth and the range of PDT action depths basing on the red-to-blue signal ratio dynamics R λ ¼ I f660 ∕I f405 , where I f660 and I f405 are the fluorescence intensities averaged over the region of interest upon excitation at wavelengths of 660 and 405 nm, respectively.…”

Section: Optical Diagnostics Modalitiesmentioning

confidence: 99%

Comparative analysis of single- and dual-wavelength photodynamic therapy regimes with chlorin-based photosensitizers: animal study

Kurakina¹,

Khilov²,

Shakhova

et al. 2019

J. Biomed. Opt.

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Comparative analysis of single-and dual-wavelength photodynamic therapy regimes with chlorin-based photosensitizers: animal study," J.Abstract. Two pronounced absorption peaks in blue and red ranges of the chlorin-based photosensitizer (PS) absorption spectrum provide additional benefits in photodynamic therapy (PDT) performance. Differing optical properties of biological tissues in these ranges allow for both dual-wavelength diagnostics and PDT performance. We provide a comparative analysis of different PDT regimes performed with blue and red lights and their combination, with doses varying from 50 to 150 J∕cm 2 . The study was performed on the intact skin of a rabbit ear inner surface, with the use of chlorin e6 as a PS. PDT procedure protocol included monitoring of the treated site with fluorescence imaging technique to evaluate PS accumulation and photobleaching, as well as with optical coherence tomography (OCT) to register morphological and functional responses of the tissue. Optical diagnostic observations were compared with the results of histopathology examination. We demonstrated that PDT procedures with the considered regimes induce weaker organism reaction manifested by edema in normal tissue as compared to irradiation-only exposures with the same light doses. The light doses delivered with red light induce weaker tissue reaction as compared to the same doses delivered with blue light only or with a combination of red and blue lights in equal parts. Results of in-vivo OCT monitoring of tissue reaction are in agreement with the results of histopathology study.

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“…The irradiance at the tissue area was tuned to 0.4 W∕cm 2 resulting in total illumination time of 125 s. The treated area was 2 × 3 cm 2 , a total of six areas were treated. Optical monitoring of a PDT procedure was performed by OCT (OCT-1300E device, BioMedTech, IAP RAS, Nizhny Novgorod, Russia) and FI (fluorescence imaging device, 50 IAP RAS, Nizhny Novgorod, Russia). OCT imaging was performed before PS administration (intact tissue), in 15 min after PS administration, immediately after laser impact, in 1, 3, and 7 days after PDT procedure.…”

Section: Animal Studymentioning

confidence: 99%

Photodynamic therapy with chlorin-based photosensitizer at 405 nm: numerical, morphological, and clinical study

et al. 2018

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Employment of chlorin-based photosensitizers (PSs) provides additional advantages to photodynamic therapy (PDT) due to absorption peak around 405 nm allowing for superficial impact and efficient antimicrobial therapy. We report on the morphological and clinical study of the efficiency of PDT at 405 nm employing chlorin-based PS. Numerical studies demonstrated difference in the distribution of absorbed dose at 405 nm in comparison with traditionally employed wavelength of 660 nm and difference in the in-depth absorbed dose distribution for skin and mucous tissues. Morphological study was performed at the inner surface of rabbit ear with histological examinations at different periods after PDT procedure. Animal study revealed tissue reaction to PDT consisting in edema manifested most in 3 days after the procedure and neoangiogenesis. OCT diagnostics was confirmed by histological examination. Clinical study included antimicrobial PDT of pharynx chronic inflammatory diseases. It revealed no side effects or complications of the PDT procedure. Pharyngoscopy indicated reduction of inflammatory manifestations, and, in particular cases, hypervascularization was observed. Morphological changes were also detected in the course of monitoring, which are in agreement with pharyngoscopy results. Microbiologic study after PDT revealed no pathogenic bacteria; however, in particular cases, saprophytic flora was detected.

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Compact and fully automated system for monitoring photodynamic therapy, based on two LEDs and a single CCD

Cited by 21 publications

References 25 publications

Dual-Wavelength Fluorescence Monitoring of Photodynamic Therapy: From Analytical Models to Clinical Studies

Dual-Wavelength Fluorescence Monitoring of Photodynamic Therapy: From Analytical Models to Clinical Studies

Comparative analysis of single- and dual-wavelength photodynamic therapy regimes with chlorin-based photosensitizers: animal study

Photodynamic therapy with chlorin-based photosensitizer at 405 nm: numerical, morphological, and clinical study

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