Community Structure and Potential Nitrogen Metabolisms of Subtropical Aquaculture Pond Microbiota

Abstract

Microorganisms play a substantial role in the aquatic nitrogen cycle. However, the community structures and metabolic potential of microbiota inhabiting subtropical aquaculture ponds are still poorly understood. In the present study, we investigated the community structure of pond microbiota by amplicons sequencing using the MiSeq sequencing platform and predicted their potential metabolic characteristics. A total of 614 dominant operational taxonomic units (OTUs) were detected from 27 samples, of which 61 were dominant in more than half of the samples. Many of the taxa observed in this study have known roles in nitrification and denitrification. In addition, most genes related to nitrogen metabolism were detected using a predictive metagenome approach, and a complementary pattern in which ponds had different genes in the same metabolic pathway emerged. These results provide basic information for the management of microbiota to control the nitrogen balance in pond water.

Peer Review #2 of "Artificial substrata increase pond farming density of grass carp (Ctenopharyngodon idella) by increasing the bacteria that participate in nitrogen and phosphorus cycles in pond water (v0.1)"

“…The accumulation of TN and TP in pond water is considered an important reason for slower growth rates in aquaculture (Rahman et al, 2005;Besson et al, 2016;Ni et al, 2018). In the present study, our results showed that TN and TP in pond water fluctuated but increased with increases in aquaculture time ( Fig.…”

Effects of Dietary Andrographolide Levels on Growth Performance, Antioxidant Capacity, Intestinal Immune Function and Microbioma of Rice Field Eel (Monopterus Albus)

“…The mRNA primer sequences are listed in Table 2 , and RPL-17 was selected as a reference gene. Expression was calculated using the Ct (2 −ΔΔCt ) method [ 35 , 36 , 37 ].…”

Peer Review #1 of "Artificial substrata increase pond farming density of grass carp (Ctenopharyngodon idella) by increasing the bacteria that participate in nitrogen and phosphorus cycles in pond water (v0.1)"

“…High-density culture in ponds not only reduces the growth rate of cultured fish, but also increases their FCR, and increases the accumulation of TN and TP in pond water and sediment (Rahman et al, 2005;Besson et al, 2016). The accumulation of TN and TP, and especially the increase in nitrite and ammonia nitrogen, will lead to the slow growth of cultured fish (Ni et al, 2018). In the present study, our results also showed that the growth rate of grass carp decreased gradually (Fig.…”