Community epidemiology of Chlamydia and Mycoplasma pneumoniae in LRTI in France over 29 months

Gaillat, J.; Flahault, Antoine; deBarbeyrac, Bertille; Orfila, J; Portier, H.; Ducroix, J.P.; Bébéar, Christiane; Mayaud, C

doi:10.1007/s10654-005-5868-9

Cited by 19 publications

(9 citation statements)

References 35 publications

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“…As shown in these tables, the proportion of LRTI in children and adults, including community-acquired pneumonia, associated with M. pneumoniae infection during the past 5 years has ranged from 0 to 66.7% [7,8], varying with age and the geographic location of the population examined and the diagnostic methods used. In a lot of studies, the diagnosis of M. pneumoniae was based on serology alone [7,[9][10][11]; some used a PCR assay alone [8,12,13] or at least one serological test and a PCR assay [14][15][16][17][18][19][20]. Only a limited number of studies used culture in combination with a serological test and/or PCR assay [21][22][23][24][25] and three studies applied two different PCR assays [26][27][28].…”

Section: Epidemiology Of Respiratory Infection Due To M Pneumoniaementioning

confidence: 99%

Acute respiratory infection due to Mycoplasma pneumoniae: current status of diagnostic methods

Loens

Goossens

Ieven

2010

Eur J Clin Microbiol Infect Dis

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Because of the absence of well-standardized both in-house and FDA-approved commercially available diagnostic tests, the reliable diagnosis of respiratory infection due to Mycoplasma pneumoniae remains difficult. In addition, no formal external quality assessment schemes which would allow to conclude about the performance of M. pneumoniae diagnostic tests exist. In this review, the current state of knowledge of M. pneumoniae-associated respiratory infections in the context of epidemiological studies published during the past 5 years is discussed, with particular emphasis on the diagnostic strategies used and their impact on results. The role of M. pneumoniae as a cause of respiratory tract infections (RTIs) differs from study to study due to geographical and epidemiological differences, as well as to the application of different diagnostic techniques and criteria used.

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Section: Epidemiology Of Respiratory Infection Due To M Pneumoniaementioning

confidence: 99%

Acute respiratory infection due to Mycoplasma pneumoniae: current status of diagnostic methods

Loens

Goossens

Ieven

2010

Eur J Clin Microbiol Infect Dis

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“…Severe pneumonia remains the major reasons for admission to the intensive care unit (ICU), mainly related to Streptococcus pneumoniae (SP). Atypical pneumonia (AP) related, for instance, to Chlamydophila pneumoniae (CP) and Mycoplasma pneumoniae (MP) accounts for 1-30% of documented pneumonia in patients admitted to ICU [1][2][3][4][5][6][7][8][9][10][11]. Although AP is rarely severe, some patients with community-acquired AP require ICU admission.…”

Section: Introductionmentioning

confidence: 99%

Severe atypical pneumonia in critically ill patients: a retrospective multicenter study

Valade

Biard

Lemiale

et al. 2018

Ann. Intensive Care

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BackgroundChlamydophila pneumoniae (CP) and Mycoplasma pneumoniae (MP) patients could require intensive care unit (ICU) admission for acute respiratory failure. MethodsAdults admitted between 2000 and 2015 to 20 French ICUs with proven atypical pneumonia were retrospectively described. Patients with MP were compared to Streptococcus pneumoniae (SP) pneumonia patients admitted to ICUs. ResultsA total of 104 patients were included, 71 men and 33 women, with a median age of 56 [44–67] years. MP was the causative agent for 76 (73%) patients and CP for 28 (27%) patients. Co-infection was documented for 18 patients (viruses for 8 [47%] patients). Median number of involved quadrants on chest X-ray was 2 [1–4], with alveolar opacities (n = 61, 75%), interstitial opacities (n = 32, 40%). Extra-pulmonary manifestations were present in 34 (33%) patients. Mechanical ventilation was required for 75 (72%) patients and vasopressors for 41 (39%) patients. ICU length of stay was 16.5 [9.5–30.5] days, and 11 (11%) patients died in the ICU. Compared with SP patients, MP patients had more extensive interstitial pneumonia, fewer pleural effusion, and a lower mortality rate [6 (8%) vs. 17 (22%), p = 0.013]. According MCA analysis, some characteristics at admission could discriminate MP and SP. MP was more often associated with hemolytic anemia, abdominal manifestations, and extensive chest radiograph abnormalities. SP-P was associated with shock, confusion, focal crackles, and focal consolidation.ConclusionIn this descriptive study of atypical bacterial pneumonia requiring ICU admission, mortality was 11%. The comparison with SP pneumonia identified clinical, laboratory, and radiographic features that may suggest MP or CP pneumonia.Electronic supplementary materialThe online version of this article (10.1186/s13613-018-0429-z) contains supplementary material, which is available to authorized users.

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“…The highest incidence of C. pneumoniae and M. pneumoniae infections is among schoolchildren 5 to 14 years old (9,16,17,23). Symptoms can be mild with nonproductive persistent cough, malaise, and fever, but more-severe illness occurs when the lower respiratory tracts are affected, giving rise to acute bronchitis and pneumonia (3,10,14). Cardiovascular disease and neurological diseases are serious nonrespiratory symptoms associated with C. pneumoniae and M. pneumoniae infections (4,5,11,13,28).…”

mentioning

confidence: 99%

Simultaneous Detection ofChlamydophila pneumoniaeandMycoplasma pneumoniaeby Use of Molecular Beacons in a Duplex Real-Time PCR

Gullsby

Storm

2008

J Clin Microbiol

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A real-time PCR was designed for detection of Chlamydophila pneumoniae and Mycoplasma pneumoniae such that each pathogen could be detected in a single tube and differentiated using molecular beacons marked with different fluorochromes. This duplex PCR, targeting the P1 adhesion gene for M. pneumoniae and the ompA gene for C. pneumoniae, was compared with two conventional PCR assays targeting the 16S rRNA gene and the ompA gene. A total of 120 clinical throat and nasopharyngeal swab samples were tested. DNA extraction was performed using an alkali denaturation/neutralization method, and real-time amplification, detection, and data analysis were performed using a Rotor-Gene 2000 real-time rotary analyzer (Corbett Life Science, Sydney, Australia). Using conventional PCR as a reference in an analysis of 120 samples, 13 of 14 samples positive for C. pneumoniae were detected by the novel real-time PCR. In an analysis of M. pneumoniae, 22 samples were positive in the conventional PCR and the novel assay detected 24 positive samples. When using the conventional PCR as a reference, sensitivity and specificity were 93% and 100%, respectively, for C. pneumoniae and 100% and 98%, respectively, for M. pneumoniae. With an overall agreement of 98.8%, this suggests that performance of the new duplex real-time PCR is comparable to that of conventional PCR.

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Community epidemiology of Chlamydia and Mycoplasma pneumoniae in LRTI in France over 29 months

Abstract: CP or MP diagnosed by PCR were found in more than 7% of patients with LRTI in community settings with a significant geographical heterogeneity and significant temporal trends in the incidence.

Cited by 19 publications

References 35 publications

Acute respiratory infection due to Mycoplasma pneumoniae: current status of diagnostic methods

Acute respiratory infection due to Mycoplasma pneumoniae: current status of diagnostic methods

Severe atypical pneumonia in critically ill patients: a retrospective multicenter study

Simultaneous Detection ofChlamydophila pneumoniaeandMycoplasma pneumoniaeby Use of Molecular Beacons in a Duplex Real-Time PCR

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