Community effectiveness of pyriproxyfen as a dengue vector control method: A systematic review

Maoz, Dorit; Ward, Tara; Samuel, Moody; Müller, Pie; Runge-Ranzinger, Silvia; Toledo, João; Boyce, Ross M.; Velayudhan, Raman; Horstick, Olaf

doi:10.1371/journal.pntd.0005651

Cited by 51 publications

(50 citation statements)

References 31 publications

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“…In our study, the diluted 0.01% PPF commercial product could be directly applied for residual spraying without any pretreatment. On the other hand, the concentration of PPF product used in previous autodissemination studies varied from 0.5-10% and could result in larval/pupal mortality of the dengue vector of more than 60% [9,11,21,24,30]. The concentration of PPF product used in our study (0.01%) was relatively low compared to that in other studies, and the result showed that the mean EI% was 37% one month post-spraying.…”

Section: Discussioncontrasting

confidence: 65%

Using UPLC–MS/MS to Evaluate the Dissemination of Pyriproxyfen by Aedes Mosquitoes to Combat Cryptic Larval Habitats after Source Reduction in Kaohsiung in Southern Taiwan

Chen

Lai

et al. 2020

Insects

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The policy regarding mosquito control strategies in Taiwan is based on integrated vector management (IVM). The major approach is source reduction via collaboration by both residents and governments. However, small and cryptic habitats of dengue vectors are hard to find and eliminate in urban communities. Therefore, this study evaluated a complementary approach that targeted cryptic habitats by utilizing mosquitoes themselves as vehicles to transfer an insect growth regulator, pyriproxyfen (PPF), to their breeding sites; the amount of PPF in breeding water was determined with ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). A bioassay conducted by introducing ten late-instar larvae into PPF solution was performed to assess emergence inhibition (EI). PPF was found at 0.56 ± 0.04 ng in 25 mL of water by dissemination via ten Aedes aegypti mosquitoes exposed to 0.01% PPF, leading to 100% EI. After the community-level source reduction, a field trial in Kaohsiung in Southern Taiwan showed that 30.8-31.5% of cryptic ovitraps reached EI ≥ 50% one month after spraying 0.01% PPF in microhabitats favored by mosquitoes. IVM in parallel with residual spraying of PPF on resting surfaces of mosquitoes could serve as a simple and complementary approach to reduce cryptic larval sources in urban communities in Southern Taiwan.

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Section: Discussioncontrasting

confidence: 65%

Using UPLC–MS/MS to Evaluate the Dissemination of Pyriproxyfen by Aedes Mosquitoes to Combat Cryptic Larval Habitats after Source Reduction in Kaohsiung in Southern Taiwan

Chen

Lai

et al. 2020

Insects

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“…Novel vector control methods include lethal ovitraps [ 18 ], insect growth regulators ( e . g ., pyroproxyfen) [ 19 ], Wolbachia infections [ 20 ], and genetically-modified sterile mosquitoes [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Social-ecological factors and preventive actions decrease the risk of dengue infection at the household-level: Results from a prospective dengue surveillance study in Machala, Ecuador

et al. 2017

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BackgroundIn Ecuador, dengue virus (DENV) infections transmitted by the Aedes aegypti mosquito are among the greatest public health concerns in urban coastal communities. Community- and household-level vector control is the principal means of controlling disease outbreaks. This study aimed to assess the impact of knowledge, attitudes, and practices (KAPs) and social-ecological factors on the presence or absence of DENV infections in the household.MethodsIn 2014 and 2015, individuals with DENV infections from sentinel clinics in Machala, Ecuador, were invited to participate in the study, as well as members of their household and members of four neighboring households located within 200 meters. We conducted diagnostic testing for DENV on all study participants; we surveyed heads of households (HOHs) regarding demographics, housing conditions and KAPs. We compared KAPs and social-ecological factors between households with (n = 139) versus without (n = 80) DENV infections, using bivariate analyses and multivariate logistic regression models with and without interactions.ResultsSignificant risk factors in multivariate models included proximity to abandoned properties, interruptions in piped water, and shaded patios (p<0.05). Significant protective factors included the use of mosquito bed nets, fumigation inside the home, and piped water inside the home (p<0.05). In bivariate analyses (but not multivariate modeling), DENV infections were positively associated with HOHs who were male, employed, and of younger age than households without infections (p<0.05). DENV infections were not associated with knowledge, attitude, or reported barriers to prevention activities.DiscussionSpecific actions that can be considered to decrease the risk of DENV infections in the household include targeting vector control in highly shaded properties, fumigating inside the home, and use of mosquito bed nets. Community-level interventions include cleanup of abandoned properties, daily garbage collection, and reliable piped water inside houses. These findings can inform interventions to reduce the risk of other diseases transmitted by the Ae. aegypti mosquito, such as chikungunya and Zika fever.

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“…The inhibitory effect of PPF on mosquito reproduction is affected by the time of exposure, formulation, methods of application, and other abiotic factors [36][37][38]. In this study, we exposed adult mosquitoes to PPF by placing them into cartons that had a lining of PPF-treated cotton gauzes.…”

Section: Ppf Suppressed Reproduction Of Female Ae Aegypti In a Dose-mentioning

confidence: 99%

The juvenile hormone receptor Methoprene-tolerant is involved in the sterilizing effect of pyriproxyfen on adultAedes aegyptimosquitoes

Ahmed

Saunders

Mullins

et al. 2020

Preprint

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Exposure of adult mosquitoes to pyriproxyfen (PPF), an analog of insect juvenile hormone (JH), has shown promise to effectively sterilize female mosquitoes. However, the underlying mechanisms of the PPF-induced decrease in mosquito fecundity are largely unknown. We performed a comprehensive study to dissect the mode of PPF action in Aedes aegypti mosquitoes. Exposure to PPF prompted the overgrowth of primary follicles in sugar-fed Ae.aegypti females but blocked the development of primary follicles at Christopher's Stage III after blood feeding. Secondary follicles were precociously activated in PPF-treated mosquitoes.Moreover, PPF substantially altered the expression of many genes that are essential for mosquito physiology and oocyte development in the fat body and ovary. In particular, many metabolic genes were differentially expressed in response to PPF treatment, thereby affecting the mobilization and utilization of energy reserves. Furthermore, PPF treatment on the previtellogenic female adults considerably modified mosquito responses to JH and 20hydroxyecdysone (20E), two major hormones that govern mosquito reproduction. Krüppel homolog 1, a JH-inducible transcriptional regulator, showed consistently elevated expression after PPF exposure. Conversely, PPF upregulated the expression of several key players of the 20E regulatory cascades, including HR3 and E75A, in the previtellogenic stage. After bloodfeeding, the expression of these 20E response genes was significantly weaker in PPF-treated mosquitoes than the solvent-treated control groups. RNAi-mediated knockdown of the Methoprene-tolerant (Met) protein, the JH receptor, partially rescued the impaired follicular development after PPF exposure and substantially increased the hatching of the eggs produced by PPF-treated female mosquitoes. Thus, the results suggested that PPF relied on Met to exert its sterilizing effects on female mosquitoes. In summary, this study finds that PPF exposure disturbs normal hormonal responses and metabolism in Ae. aegypti, shedding light on the molecular targets and the downstream signaling pathways activated by PPF. Author summaryAedes aegypti mosquitoes are responsible for the transmission of dengue, yellow fever, chikungunya, and Zika fever. Insecticides are widely used as the primary tool in the prevention and control of these infectious diseases. In light of the rapid increase of insecticide resistance in mosquito populations, there is an urgent need to find new classes of insecticides with a different mode of action. Here we found that pyriproxyfen, an analog of insect juvenile hormone (JH), had a large impact on the oocyte development, both before and after blood feeding, in female mosquitoes. Pyriproxyfen disturbed normal hormonal responses and caused metabolic shifting in female adults. These actions appear to collectively impair oocyte development and substantially reduce viable progenies of female mosquitoes. Besides, we demonstrated the involvement of the JH receptor Met in pyriproxyfen-induced female sterilization. ...

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Community effectiveness of pyriproxyfen as a dengue vector control method: A systematic review

Cited by 51 publications

References 31 publications

Using UPLC–MS/MS to Evaluate the Dissemination of Pyriproxyfen by Aedes Mosquitoes to Combat Cryptic Larval Habitats after Source Reduction in Kaohsiung in Southern Taiwan

Using UPLC–MS/MS to Evaluate the Dissemination of Pyriproxyfen by Aedes Mosquitoes to Combat Cryptic Larval Habitats after Source Reduction in Kaohsiung in Southern Taiwan

Social-ecological factors and preventive actions decrease the risk of dengue infection at the household-level: Results from a prospective dengue surveillance study in Machala, Ecuador

The juvenile hormone receptor Methoprene-tolerant is involved in the sterilizing effect of pyriproxyfen on adultAedes aegyptimosquitoes

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