Community-associated methicillin-resistant Staphylococcus aureus (MRSA) in Australia

Nimmo, Graeme R.; Coombs, Geoffrey W.

doi:10.1016/j.ijantimicag.2007.08.011

Cited by 109 publications

(107 citation statements)

References 49 publications

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“…PVL-negative reference strains were Sanger MSSA476 (a sequenced ST1-MSSA-SCCfus, i.e., with a staphylococcal casette chromosome element harboring fusC; GenBank accession number BX571857.1), Mu50 and N315 (both sequenced ST5-MRSA-II strains; GenBank accession numbers BA000017.4 and BA000018.3), NCTC 8325 (a sequenced ST8-MSSA strain; GenBank accession number CP000253.1), and COL (a sequenced CC8/ST250-MRSA-I isolate; GenBank accession number CP000046.1), as well as West Australian (WA) MRSA-8 (ST75-MRSA-IV 03-17848 [19]) and WA-MRSA-59 (a CC12-MRSA strain with an atypical staphylococcal cassette chromosome mec element [SCCmec] [20]). …”

Section: Methodsmentioning

confidence: 99%

“…PVL-positive reference strains were MW2-USA400 (a sequenced ST1-MRSA-IV strain; GenBank accession number BA000033.2), USA300-FPR3757 (a sequenced ST8-MRSA-IV strain; GenBank accession number CP000255.1), ATCC 25923 (a historic ST30-MSSA isolate widely used in diagnostic microbiology for quality control purposes [21]), Queensland CA-MRSA (ST93-MRSA-IV 03-16790 [19]), and WA-MRSA-60/Bengal Bay CA-MRSA (ST772-MRSA-V [20]). …”

Section: Methodsmentioning

confidence: 99%

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Rapid Detection of Panton-Valentine Leukocidin in Staphylococcus aureus Cultures by Use of a Lateral Flow Assay Based on Monoclonal Antibodies

Monecke

Müller

Buechler³

et al. 2013

J Clin Microbiol

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Panton-Valentine leukocidin (PVL) is a virulence factor of Staphylococcus aureus, which is associated with skin and soft-tissue infections and necrotizing pneumonia. To develop a rapid phenotypic assay, recombinant PVL F component was used to generate monoclonal antibodies by phage display. These antibodies were spotted on protein microarrays and screened using different lukF-PV preparations and detection antibodies. This led to the identification of the optimal antibody combination that was then used to establish a lateral flow assay. This test was used to detect PVL in S. aureus cultures. The detection limit of the assay with purified native and recombinant antigens was determined to be around 1 ng/ml. Overnight cultures from various solid and liquid media proved suitable for PVL detection. Six hundred strains and clinical isolates from patients from America, Europe, Australia, Africa, and the Middle East were tested. Isolates were genotyped in parallel by DNA microarray hybridization for confirmation of PVL status and assignment to clonal complexes. The sensitivity, specificity, and positive and negative predictive values of the assay in this trial were 99.7, 98.3, 98.4, and 99.7%, respectively. A total of 302 clinical isolates and reference strains were PVL positive and were assigned to 21 different clonal complexes. In summary, the lateral flow test allows rapid and economical detection of PVL in a routine bacteriology laboratory. As the test utilizes cultures from standard media and does not require sophisticated equipment, it can be easily integrated into a laboratory's workflow and might contribute to timely therapy of PVLassociated infections.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Rapid Detection of Panton-Valentine Leukocidin in Staphylococcus aureus Cultures by Use of a Lateral Flow Assay Based on Monoclonal Antibodies

Monecke

Müller

Buechler³

et al. 2013

J Clin Microbiol

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“…The prevailing genotype in the current study, PFGE type D, was identified among patients treated in six different wards over a 3-year period. Interestingly, all PFGE type D isolates harbored type IV SCCmec, which presently is found in the majority of methicillin-resistant Staphylococcus aureus (MRSA) strains of community origin (15). Furthermore, MLST results showed that four of the six identified genotypes (PFGE types D, F, G, and H) belonged to ST2.…”

mentioning

confidence: 99%

A Multidrug-Resistant Staphylococcus epidermidis Clone (ST2) Is an Ongoing Cause of Hospital-Acquired Infection in a Western Australian Hospital

et al. 2012

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Staphylococcus epidermidis is an important pathogen involved in health care-associated bloodstream infections and infections related to vascular catheters and prosthetic devices (17). Several investigations have demonstrated that certain multidrug-resistant S. epidermidis (MDRSE) genotypes become established as opportunistic pathogens in the health care setting as a novel ecological niche (8,10,12). In addition, recent studies identified several worldwide epidemic clonal lineages (9,12,13,18,21). Currently, little information is available on the molecular epidemiology of S. epidermidis in the health care setting in Australia (16,18).We have previously documented the occurrence and potential dissemination of two genotypes of MDRSE in 11 hospitals in northern Europe between 2001 and 2008. The aims of this study were to examine the molecular epidemiology of clinical isolates of MDRSE collected in a teaching hospital in Australia and determine the possible presence of previously described health careassociated MDRSE clones.

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“…The CA-MRSA clones included three international PVL-positive clones; USA300 (one isolate), Taiwan CA-MRSA (one isolate) and South West Pacific (SWP CA-MRSA) (two isolates). The remaining eight CA-MRSA clones included the five dominant CA-MRSA clones isolated in Australia [11]; PVLpositive Queensland (Qld) CA-MRSA (ST93-MRSA-IV), WA1 CA-MRSA (ST1-MRSA-IV), of which two isolates were PVL positive, WA23 CA-MRSA (ST45-MRSA-IV), WA2 CA-MRSA (ST78-MRSA-IV), and WA3 CA-MRSA (ST5-MRSA-IV). Penicillin Study ID 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 …”

Section: Clonal Diversitymentioning

confidence: 99%

“…Although S. aureus possesses a suite of virulence factors, most interest has been invested in one major determinant, Panton-Valentine leukocidin (PVL), because emergent virulent community-acquired MRSA isolates in the USA, Europe and Australia are frequently PVL positive [9][10][11]. However, the exact role that PVL plays in pathogenesis remains a topic of intense controversy [12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Community-onsetStaphylococcus aureusinfections presenting to general practices in South-eastern Australia

et al. 2013

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SUMMARYCommunity-acquired Staphylococcus aureus infections are a public health concern, yet little is known about infections that do not present to hospital. We identified community-onset S. aureus infections via specimens submitted to a community-based pathology service. Referring doctors confirmed eligibility and described infection site, severity and treatment. Isolates were characterized on antibiotic resistance, PFGE, MLST/SCCmec, and Panton-Valentine leukocidin (PVL), representing 106 community-onset infections; 34 non-multiresistant methicillin-resistant S. aureus (nmMRSA) (resistant to <3 non-β-lactam antibiotics), 15 multiply antibiotic-resistant MRSA (mMRSA) and 57 methicillin-sensitive S. aureus (MSSA). Most (93%) were skin and soft tissue infections. PVL genes were carried by 42% of nmMRSA isolates [95% confidence interval (CI) 26-61] and 15% of MSSA (95% CI 8-28). PVL was associated with infections of the trunk, head or neck (56·4% vs. 24·3%, P = 0·005) in younger patients (23 vs. 52 years, P < 0·001), and with boils or abscesses (OR 8·67, 95% CI 2·9-26·2), suggesting underlying differences in exposure and/or pathogenesis.

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Community-associated methicillin-resistant Staphylococcus aureus (MRSA) in Australia

Cited by 109 publications

References 49 publications

Rapid Detection of Panton-Valentine Leukocidin in Staphylococcus aureus Cultures by Use of a Lateral Flow Assay Based on Monoclonal Antibodies

Rapid Detection of Panton-Valentine Leukocidin in Staphylococcus aureus Cultures by Use of a Lateral Flow Assay Based on Monoclonal Antibodies

A Multidrug-Resistant Staphylococcus epidermidis Clone (ST2) Is an Ongoing Cause of Hospital-Acquired Infection in a Western Australian Hospital

Community-onsetStaphylococcus aureusinfections presenting to general practices in South-eastern Australia

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