Communication—Accessing Stability of Oxidase-Based Biosensors via Stabilizing the Advanced H₂O₂Transducer

Abstract

Operational stability of biosensors is of particular importance especially for wearable devices. Prussian Blue (PB) based advanced hydrogen peroxide transducer, 1000 times more active and selective than platinum, is deposited onto screen-printed structures and stabilized with nickel hexacyanoferrate (NiHCF), both in open circuit mode. Operational stability of PB-NiHCF bilayer based biosensors and labile lactate oxidase is significantly improved in terms of twice longer half inactivation and ≈3.5 times lower inactivation constant. The dynamic range of PB-NiHCF based biosensors is similar to it for conventional PB based ones, which allows using the former for similar purposes drastically improving their performance characteristics.

Electrochromic biosensors based on screen-printed Prussian Blue electrodes

“…ferrocyanide is also possible and may be desirable when a less acidic pH is required to preserve the activity of enzymes or other biomolecules. [15] Chemical growth, on the other hand, may also be used to produce Prussian Blue films, both on electrodes [26,30,31] and on inert surfaces [32] in many different ways. [33] However, this is not an ideal approach because it lacks the surface selectivity and much of the control that electrochemical methods enjoy.…”

Assessment of MgZr4P6O24as a Solid Electrolyte for Sensing Mg in Molten Non-Ferrous Alloys

“…21 Operational stability of the oxide-based electrochemical sensor characterised in this study, like the oxidase-based biosensors, is of particular importance as it significantly improves the sensor performance and applicability in the relevant environment. 22 One requirement for electromotive force (emf) measurements is that both the electrolyte and electrodes are chemically stable in the operating environment. 23 Thus, emf measurements can be conveniently used to measure the more active element in an alloy, as illustrated in this study where the activity of Mg has been measured in molten Al.…”

Physicochemical Characteristics of Polypyrrole/(Glucose oxidase)/(Prussian Blue)‐based Biosensor Modified with Ni‐ and Co‐Hexacyanoferrates

“…Enzymes‐oxidases‐oxidize their specific substrates in the presence of molecular oxygen and forms hydrogen peroxide as one of reaction product . Therefore, the application of different metal hexacyanoferrates in modified electrodes has been extensively studied and these materials are becoming used as redox mediators in biosensors based on oxidase‐type enzymes as the biological recognition element.…”

“…However, the mechanism of hydrogen peroxide reduction catalysis by transition metals hexacyanoferrates is not discovered yet . Moreover, it is claimed that the electrocatalytic activity of non‐iron transition metals hexacyanoferrates occurs due to presence of some Prussian blue in the crystal lattice of these hexacyanoferrates . Recently Sitnikova et.…”

“…Even though composite Prussian blue‐non‐iron metal hexacyanoferrate layers have been created successfully , according to the best of our knowledge, glucose biosensor containing composite Prussian blue/non‐iron metal hexacyanoferrate (PB‐Me non‐iron HCF) layer have not been reported yet. An important advantage in the formation of PB‐Me non‐iron HCF layer is simplicity of deposition procedure.…”