Communicating by touch – neurons are not alone

Kornberg, Thomas B.; Roy, Sougata

doi:10.1016/j.tcb.2014.01.003

Cited by 65 publications

(61 citation statements)

References 58 publications

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“…This was caused by the changing morphology of developing neurons forming long processes, which led to increased intercellular space within the network, and by reduced tight cell-cell contacts accompanying progenitor-to-neuron transition (Kornberg and Roy 2014). In contrast, R Cell of the control (NPM) increased until day 8 followed by a decrease till day 14 to values still higher than found at day 0.…”

Section: Reliable and Sensitive Impedimetric Monitoring Of Neural Plumentioning

confidence: 97%

Impedimetric real-time monitoring of neural pluripotent stem cell differentiation process on microelectrode arrays

Seidel

Obendorf

Englich

et al. 2016

Biosensors and Bioelectronics

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In today's neurodevelopment and -disease research, human neural stem/progenitor cell-derived networks represent the sole accessible in vitro model possessing a primary phenotype. However, cultivation and moreover, differentiation as well as maturation of human neural stem/progenitor cells are very complex and time-consuming processes. Therefore, techniques for the sensitive non-invasive, real-time monitoring of neuronal differentiation and maturation are highly demanded. Using impedance spectroscopy, the differentiation of several human neural stem/progenitor cell lines was analyzed in detail. After development of an optimum microelectrode array for reliable and sensitive long-term monitoring, distinct cell-dependent impedimetric parameters that could specifically be associated with the progress and quality of neuronal differentiation were identified. Cellular impedance changes correlated well with the temporal regulation of biomolecular progenitor versus mature neural marker expression as well as cellular structure changes accompanying neuronal differentiation. More strikingly, the capability of the impedimetric differentiation monitoring system for the use as a screening tool was demonstrated by applying compounds that are known to promote neuronal differentiation such as the γ-secretase inhibitor DAPT. The non-invasive impedance spectroscopy-based measurement system can be used for sensitive and quantitative monitoring of neuronal differentiation processes. Therefore, this technique could be a very useful tool for quality control of neuronal differentiation and moreover, for neurogenic compound identification and industrial high-content screening demands in the field of safety assessment as well as drug development.

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Section: Reliable and Sensitive Impedimetric Monitoring Of Neural Plumentioning

confidence: 97%

Impedimetric real-time monitoring of neural pluripotent stem cell differentiation process on microelectrode arrays

Seidel

Obendorf

Englich

et al. 2016

Biosensors and Bioelectronics

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“…1,2,4 A large number of experimental and theoretical investigations on the formation and functioning of morphogen gradients has appeared in recent years. [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]21,22 However, many aspects of biological signaling remain not well understood. 4,6 One of the most important problems of the biological development is to understand the mechanisms of the morphogen gradients formation.…”

Section: Figure 0: Toc Graphicmentioning

confidence: 99%

New Model for Understanding Mechanisms of Biological Signaling: Direct Transport via Cytonemes

Teimouri

Kolomeisky

2015

J. Phys. Chem. Lett.

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Biological signaling is a crucial natural process that governs the formation of all multicellular organisms. It relies on efficient and fast transfer of information between different cells and tissues. It has been presumed for a long time that these long-distance communications in most systems can take place only indirectly via the diffusion of signaling molecules, also known as morphogens, through the extracellular fluid. However, recent experiments indicate that there is also an alternative direct delivery mechanism. It utilizes dynamic tubular cellular extensions, called cytonemes, that directly connect cells, supporting the flux of morphogens to specific locations. We present a first quantitative analysis of the cytoneme-mediated mechanism of biological signaling. Dynamics of the formation of signaling molecule profiles, which are also known as morphogen gradients, is discussed. It is found that the direct-delivery mechanism is more robust with respect to fluctuations in comparison with the passive diffusion mechanism. In addition, we show that the direct transport of morphogens through cytonemes

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“…3B). Cytonemes make synaptic contacts and ferry signaling proteins between signaling cells (Kornberg and Roy, 2014a, b; Roy et al, 2014). They are actin-based filopodia and do not contain microtubules (Ramírez-Weber and Kornberg, 1999).…”

Section: Textmentioning

confidence: 99%

The contrasting roles of primary cilia and cytonemes in Hh signaling

Kornberg

2014

Developmental Biology

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Hedgehog (Hh) is a paracrine signaling protein with major roles in development and disease. In vertebrates and invertebrates, Hh signal transduction is carried out almost entirely by evolutionarily conserved components, and in both, intercellular movement of Hh is mediated by cytonemes – specialized filopodia that serve as bridges that bring distant cells into contact. A significant difference is the role of primary cilia, a slender, tubulin-based protuberance of many vertebrate cells. Although the primary cilium is essential for Hh signaling in cells that have one, most Drosophila cells lack a primary cilium. This perspective addresses the roles of primary cilia and cytonemes, and proposes that for Hh signaling, the role of primary cilia is to provide a specialized hydrophobic environment that hosts lipid-modified Hh and other components of Hh signal transduction after Hh has traveled from elsewhere in the cell. Implicit in this model is the idea that initial binding and uptake of Hh is independent of and segregated from the processes of signal transduction and activation.

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Communicating by touch – neurons are not alone

Cited by 65 publications

References 58 publications

Impedimetric real-time monitoring of neural pluripotent stem cell differentiation process on microelectrode arrays

Impedimetric real-time monitoring of neural pluripotent stem cell differentiation process on microelectrode arrays

New Model for Understanding Mechanisms of Biological Signaling: Direct Transport via Cytonemes

The contrasting roles of primary cilia and cytonemes in Hh signaling

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