2019
DOI: 10.1101/530964
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Commonly used insect repellents hide human odors from Anopheles mosquitoes

Abstract: 18The mode of action for most mosquito repellents is unknown. This is primarily due to the 19 difficulty in monitoring how the mosquito olfactory system responds to repellent odors. 20Here, we used the Q-system of binary expression to enable activity-dependent Ca 2+ 21 imaging in olfactory neurons of the African malaria mosquito Anopheles coluzzii. This 22 disruptively change the chemical profile of host scent signatures on the skin surface 34 rendering humans invisible to mosquitoes. 35 36 Introduction 37Mo… Show more

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Cited by 12 publications
(16 citation statements)
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“…To build a flexible system for expressing a diverse array of effector transgenes, we employed the Q-binary expression system for transgene amplification (Potter et al, 2010), which has been successfully implemented in An. gambiae malaria mosquitoes (Riabinina et al, 2016; Afify et al, 2019). We used CRISPR-Cas9 with the same guide RNA used to generate the ppk301 mutant to introduce an in-frame T2A sequence into the ppk301 locus followed by the QF2 transcriptional activator (Figure 4B).…”
Section: Resultsmentioning
confidence: 99%
“…To build a flexible system for expressing a diverse array of effector transgenes, we employed the Q-binary expression system for transgene amplification (Potter et al, 2010), which has been successfully implemented in An. gambiae malaria mosquitoes (Riabinina et al, 2016; Afify et al, 2019). We used CRISPR-Cas9 with the same guide RNA used to generate the ppk301 mutant to introduce an in-frame T2A sequence into the ppk301 locus followed by the QF2 transcriptional activator (Figure 4B).…”
Section: Resultsmentioning
confidence: 99%
“…DEET has some drawbacks; for example, it needs to be used in high concentrations (up to 30%) for biting arthropods, which dissolves some plastics; it is oily and has an unpleasant smell; and there is the possibility of developing DEET‐insensitive mutants, such as in Drosophila melanogaster (Meigen) 12,13 . To improve and discover new repellents, some repellents, such as IR3535, pidaridin, and even natural botanical‐based insect repellents, have been studied, but the mode of action remains unclear 14 …”
Section: Introductionmentioning
confidence: 99%
“…Proof of this hypothesis will have to await the identification of the specific antagonist binding sites and detailed mutagenesis studies. The proposed mode of action for the identified bioactive VOCs is thus distinct from the receptorindependent function of DEET, which, apparently, "repels" mosquitoes and other insects, at least in part, via its association with volatile receptor ligands acting as attractants, thereby reducing their volatility and effective concentrations thus masking their presence in the mosquito's environment (51).…”
Section: Discussionmentioning
confidence: 93%