Commonly Used Drugs Impair Oral Tolerance in Mice

Pecquet, Sophie; Prioult, Guénolée; Campbell, John C.; German, Bruce; Turini, Marco

doi:10.1196/annals.1309.021

Cited by 8 publications

(7 citation statements)

References 12 publications

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“…These results contradict those presented in a recent paper, which described that depletion of the gut microbiota with oral antibiotics results not only in altered induction but also in impaired maintenance of OT to cow's milk proteins (31). These discrepancies could be attributed to major differences between the two studies.…”

Section: Discussioncontrasting

confidence: 56%

“…This impaired specific humoral response leads us to hypothesize that antibiotic treatment early in life might facilitate allergic sensitization to novel food antigens. Although these results should be validated in appropriate clinical trials, a practical recommendation resulting from them may be to avoid the introduction of new food ingredients in the infant diet during an antibiotic treatment (31) in order to reduce the risk of food allergy onset.…”

Section: Discussionmentioning

confidence: 99%

“…These discrepancies could be attributed to major differences between the two studies. Pecquet et al (31) used weaning mice, while we used suckling rats; both the strain and the age of the animals have been shown to affect the immune response. The antibiotics used in the two studies and their effects on the intestinal microbiota also differed and might also have affected the OT response.…”

Section: Discussionmentioning

confidence: 99%

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Antibiotic Administration Early in Life Impairs Specific Humoral Responses to an Oral Antigen and Increases Intestinal Mast Cell Numbers and Mediator Concentrations

Nutten

Schumann

Donnicola

et al. 2007

Clin Vaccine Immunol

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In this study, we assessed the effect of administering the antibiotic amoxicillin to rat pups on the immune response to orally fed ovalbumin (OVA). We first established that amoxicillin administration durably altered the gut microbiota of these animals. In parallel, we observed that the induction of the specific humoral response to ovalbumin was impaired when it occurred during antibiotic administration to the rat pups. We also examined the consequences of those observations on further allergic reactions. Amoxicillin administration had no significant impact on subsequent sensitization to OVA, as nonexacerbated systemic allergic responses were induced in antibiotic-treated animals. However, increased rat mast cell protease II levels and higher mast cell numbers were detected in their small intestines, independently of the antigen administration. Globally, our data suggest that antibiotic administration early in life negatively affects the specific immune response to a luminal antigen when it is first introduced during antibiotic administration. The increased mast cell numbers and mediator concentrations in the intestinal mucosae of the antibiotic-treated animals may testify to the early stages of an altered immune system homeostasis.

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Section: Discussioncontrasting

confidence: 56%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Antibiotic Administration Early in Life Impairs Specific Humoral Responses to an Oral Antigen and Increases Intestinal Mast Cell Numbers and Mediator Concentrations

Nutten

Schumann

Donnicola

et al. 2007

Clin Vaccine Immunol

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“…In addition, stromal cells constitutively express Cox-2 and produce PGE 2 (Newberry et al, 2001), which inhibits IL-12 production by human moDCs, and at low concentrations induces release of IL-23 through differential effects on PGE 2 receptors (Poloso et al, 2013). The overall effect of PGE 2 , however, is anti-inflammatory in the mouse, and contributes to oral tolerance induction (Newberry et al, 2001(Newberry et al, , 1999Pecquet et al, 2004).…”

Section: Conditioning Of Dcs In the Intestinementioning

confidence: 94%

Mucosal Dendritic Cells

2015

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“…Impairment of oral tolerance can be caused by a certain number of therapeutic agents (Kim and Ohsawa, 1995;Louis et al, 1996;Pecquet et al, 2004) and a dioxin (Chmill et al, 2010) and its implication in food allergy has been discussed. However, OVA levels in serum of mice were alike, irrespective of SA exposure (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Effective induction of oral anaphylaxis to ovalbumin in mice sensitized by feeding of the antigen with aid of oil emulsion and salicylate

et al. 2012

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INTRODUCTIONThe potential allergenicity of novel proteins introduced into genetically engineered crops and that of proteins generated during the biotechnological process of food production is an important issue for the evaluation for safety of the food crops and products. Many approaches for assessing the allergenicity of such proteins have been proposed; these can be broadly classified into four areas, namely, the analysis of protein structure to predict allergenicity, serum screening of allergic proteins, new animal models, and proteomics (Selgrade et al., 2009;Ahuja et al., 2010).The biochemical approaches for the assessment of allergenic proteins are grounded on comparisons of the serologic identity of the novel protein with human allergens, taking into account factors such as structural similarity, amino acid sequence homology, resistance to proteolytic digestion in a simulated gastric fluid, and immunochemical reactivity with serum of subjects with allergy (Ladics, 2008). Although such investigations provide important information, they do not directly assess the inherent sensitizing potential of proteins. Animal models of food allergy are required for evaluating the sensitizing potential of allergenic proteins and to investigate mechanisms of food allergy. It is generally recognized that it is hard to reproduce food allergy in animals by oral feeding of a model allergenic protein at ABSTRACT -It is important to evaluate the ability of novel proteins in food crops and products to elicit potentially harmful immunologic responses, including allergic hypersensitivity. We developed a novel mouse model of food allergy involving an oral challenge of a protein antigen after feeding of the antigen in combination with modulating factors often ingested in daily life, namely, dietary oil emulsion and salicylate. In the model, BALB/c mice were sensitized orally for three weeks with ovalbumin (OVA) in linoleic acid/lecithin emulsion, followed immediately by intraperitoneal injection of sodium salicylate. At the end of the sensitization, the incidence of mice positive for serum OVA-specific IgG1 but not IgE had significantly increased in the combined-sensitization group. After the 3-week sensitization, a single or double oral challenge with OVA effectively and significantly caused severe anaphylaxis, as compared with the groups sensitized with OVA in the emulsion or the vehicle alone. Moderate increase of plasma histamine and intestinal abnormality in histology was found only in the combined-sensitization group. Anaphylaxis symptoms in the sensitized mice were induced more by oral challenge than by intravenous challenge, suggesting a critical role for the mucosal system. This is the first model for successful induction of oral anaphylaxis in mice sensitized by feeding of food protein without adjuvant. It will be useful to elucidate the mechanism of food allergy and to detect modulating factors of oral allergy at sensitization using this model, which simulates real life conditions.

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Commonly Used Drugs Impair Oral Tolerance in Mice

Cited by 8 publications

References 12 publications

Antibiotic Administration Early in Life Impairs Specific Humoral Responses to an Oral Antigen and Increases Intestinal Mast Cell Numbers and Mediator Concentrations

Antibiotic Administration Early in Life Impairs Specific Humoral Responses to an Oral Antigen and Increases Intestinal Mast Cell Numbers and Mediator Concentrations

Mucosal Dendritic Cells

Effective induction of oral anaphylaxis to ovalbumin in mice sensitized by feeding of the antigen with aid of oil emulsion and salicylate

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