Common Virulence Factors and Genetic Relationships between O18:K1:H7
            <i>Escherichia coli</i>
            Isolates of Human and Avian Origin

Moulin-Schouleur, Maryvonne; Schouler, Catherine; Tailliez, Patrick; Kao, Mu-Rong; Brée, Annie; Germon, Pierre; Oswald, Éric; Mainil, Jacques; Blanco, Miguel; Blanco, Jorge

doi:10.1128/jcm.00548-06

Cited by 154 publications

(121 citation statements)

References 67 publications

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“…The present study involved a collection of 86 E. coli strains, some of which were the same as those reported in previously published studies (5,7,8,17,34,39,40,42) (Table 1). Sixty-three (73.3%) were obtained from human extraintestinal infections (28 from urinary tract infections [UTIs], 21 from sepsis, 12 from meningitis, 1 from intra-abdominal pus, and 1 from a wound infection), and 23 were obtained from the intestinal mucosae of patients with CD (16 strains) or ulcerative colitis (1 strain) and the intestinal mucosae of control subjects (without inflammatory bowel disease [non-IBD]) (6 strains).…”

Section: Methodsmentioning

confidence: 99%

Similarity and Divergence among Adherent-Invasive Escherichia coli and Extraintestinal Pathogenic E. coli Strains

et al. 2009

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Adherent-invasive Escherichia coli (AIEC) pathovar strains, which are associated with Crohn's disease, share many genetic and phenotypic features with extraintestinal pathogenic E. coli (ExPEC) strains, but little is known about the level of genetic similarity between the two pathovars. We aimed to determine the frequency of strains with the "AIEC phenotype" among a collection of ExPEC strains and to further search for a common phylogenetic origin for the intestinal and extraintestinal AIEC strains. The adhesion, invasion, and intramacrophage replication capabilities (AIEC phenotype) of 63 ExPEC strains were determined. Correlations between virulence genotype and AIEC phenotype and between intestinal/extraintestinal origin, serotype, and phylogroup were evaluated for the 63 ExPEC and 23 intestinal AIEC strains. Phylogenetic relationships between extraintestinal and intestinal AIEC strains were determined using multilocus sequence typing (MLST) and pulsed-field gel electrophoresis. Only four (6.35%) ExPEC strains, belonging to the O6:H1, O83:H1, and O25:H4 serotypes, were classified as having an AIEC phenotype. These strains were found to be genetically related to some intestinal AIEC strains of the same serotypes as revealed by MLST. No particular virulence gene sets correlated with the intestinal/extraintestinal origin of the strains or with the AIEC phenotype, whereas the gene sets did correlate with the serogroup. We identified two intestinal AIEC strains and one extraintestinal AIEC strain belonging to the O25:H4 serotype that also belonged to the emerging and virulent clonal group ST131. In conclusion, the ExPEC and AIEC pathovars share similar virulence gene sets, and certain strains are phylogenetically related. However, the majority of ExPEC strains did not behave like AIEC strains, thus confirming that the AIEC pathovar possesses virulence-specific features that, to date, are detectable only phenotypically.Members of the Enterobacteriaceae family, especially Escherichia coli, have been repeatedly suggested to play a role in the origin and/or perpetuation of Crohn's disease (CD). In part, this suggestion was based on the higher abundance of this bacterium in CD patients than in control subjects (4,10,20,23,28,29,32,41,48,51). Although considerable effort has been devoted to the search for intestinal pathogenic E. coli strains associated with CD, to date none of the six previously described pathovars (27) has been implicated in this condition. observed that E. coli strains with adhesion and invasion properties colonized the ileal mucosae of CD patients more frequently than those of control subjects. Darfeuille-Michaud et al. further characterized these strains and proposed a new potential E. coli pathovar associated with CD, which was designated adherent-invasive E. coli (AIEC) (10). The implication of AIEC in CD is becoming increasingly relevant because several independent studies from different countries have reported a higher prevalence of invasive E. coli in CD patients (4,17,33,34,47).The main chara...

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Section: Methodsmentioning

confidence: 99%

Similarity and Divergence among Adherent-Invasive Escherichia coli and Extraintestinal Pathogenic E. coli Strains

et al. 2009

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“…Several studies have investigated the similarities of UPEC and APEC in their serogroups, virulence genotypes and assignments to phylogenetic groups (Johnson et al, 1998(Johnson et al, , 2001aKaper et al, 2004;Mokady et al, 2005;Johnson et al, 2006a;Moulin-Schouleur et al, 2006). It has been proposed that poultry may be a candidate vehicle for E. coli capable of causing human urinary tract disease, based on research showing transmission of avian E. coli from poultry to humans or similarities between avian E. coli and UPEC (Levy et al, 1976;Linton et al, 1977;Ojeniyi, 1989;van den Bogaard et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…The common presence of a set of virulence-associated genes among APEC and UPEC strains as well as similar disease patterns and phylogenetic background indicate a genetic relationship between APEC and UPEC isolates (Kaper et al, 2004;Moulin-Schouleur et al, 2006;Ron, 2006). A few studies have investigated the expression in vivo of specific genes and the whole transcriptome of UPEC strain CFT073 in a murine urinary infection model, and the upregulation of some genes such as iron-related genes and outermembrane protein encoding genes was observed (Snyder et al, 2004(Snyder et al, , 2005Hagan & Mobley, 2007;Haugen et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Comparison of virulence factors and expression of specific genes between uropathogenic Escherichia coli and avian pathogenic E. coli in a murine urinary tract infection model and a chicken challenge model

et al. 2009

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Avian pathogenic Escherichia coli (APEC) and uropathogenic E. coli (UPEC) establish infections in extraintestinal habitats of different hosts. As the diversity, epidemiological sources and evolutionary origins of extraintestinal pathogenic E. coli (ExPEC) are so far only partially defined, in the present study,100 APEC isolates and 202 UPEC isolates were compared by their content of virulence genes and phylogenetic groups. The two groups showed substantial overlap in terms of their serogroups, phylogenetic groups and virulence genotypes, including their possession of certain genes associated with large transmissible plasmids of APEC. In a chicken challenge model, both UPEC U17 and APEC E058 had similar LD 50 , demonstrating that UPEC U17 had the potential to cause significant disease in poultry. To gain further information about the similarities between UPEC and APEC, the in vivo expression of 152 specific genes of UPEC U17 and APEC E058 in both a murine urinary tract infection (UTI) model and a chicken challenge model was compared with that of these strains grown statically to exponential phase in rich medium. It was found that in the same model (murine UTI or chicken challenge), various genes of UPEC U17 and APEC E058 showed a similar tendency of expression. Several iron-related genes were upregulated in the UTI model and/or chicken challenge model, indicating that iron acquisition is important for E. coli to survive in blood or the urinary tract. Based on these results, the potential for APEC to act as human UPEC or as a reservoir of virulence genes for UPEC should be considered. Further, this study compared the transcriptional profile of virulence genes among APEC and UPEC in vivo.

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“…Escherichia coli is a genetically diverse species that causes many infections in humans and animals such as diarrhoea, biliary and urinary tract infections, sepsis and meningitis (Johnson and Stell 2000;Moulin-Schouleur et al 2006). Some of these diseases are thought to occur as biofilm-associated infections (Reisner et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Measurement of biofilm formation by clinical isolates ofEscherichia coliis method-dependent

Naves

Prado

Huelves

et al. 2008

Journal of Applied Microbiology

166

110

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Aims: In this study, we have evaluated the impact of methodological approaches in the determination of biofilm formation by four clinical isolates of Escherichia coli in static assays. Methods and Results: The assays were performed in microtitre plates with two minimal and two enriched broths, with one‐ or two‐steps protocol, and using three different mathematical formulas to quantify adherent bacteria. Different biofilm formation patterns were found depending on the E. coli strain, culture medium and reading optical density on one‐ and two‐steps protocol. Strong or moderate biofilm formation occurred mostly in minimal media. The mathematical formulas used to quantify biofilm formation also gave different results and bacterial growth rate should be taken into account to quantify biofilm. Conclusions: Escherichia coli forms biofilms on static assays in a method‐dependent fashion, depending on strain, and it is strongly modulated by culture conditions. Significance and Impact of the Study: As verified in the studied E. coli strains, biofilm formation by any organism should be cautiously interpreted, considering all variables in the experimental settings.

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Common Virulence Factors and Genetic Relationships between O18:K1:H7 Escherichia coli Isolates of Human and Avian Origin

Cited by 154 publications

References 67 publications

Similarity and Divergence among Adherent-Invasive Escherichia coli and Extraintestinal Pathogenic E. coli Strains

Similarity and Divergence among Adherent-Invasive Escherichia coli and Extraintestinal Pathogenic E. coli Strains

Comparison of virulence factors and expression of specific genes between uropathogenic Escherichia coli and avian pathogenic E. coli in a murine urinary tract infection model and a chicken challenge model

Measurement of biofilm formation by clinical isolates ofEscherichia coliis method-dependent

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