Common variants in the genes of triglyceride and HDL-C metabolism lack association with coronary artery disease in the Pakistani subjects

Abstract

BackgroundSerum Triglyceride (TG) and High Density Lipoprotein (HDL-C) levels are modifiable coronary artery disease (CAD) risk factors. Polymorphisms in the genes regulating TG and HDL-C levels contribute to the development of CAD. The objective of the current study was to investigate the effect of four such single nucleotide polymorphism (SNPs) in the genes for Lipoprotein Lipase (LPL) (rs328, rs1801177), Apolipoprotein A5 (APOA5) (rs66279) and Cholesteryl ester transfer protein (CETP) (rs708272) on HDL-C and TG levels and to examine the association of these SNPs with CAD risk.MethodsA total of 640 subjects (415 cases, 225 controls) were enrolled in the study. The SNPs were genotyped by KASPar allelic discrimination technique. Serum HDL-C and TG were determined by spectrophotometric methods.ResultsThe population under study was in Hardy Weinberg equilibrium and minor allele of SNP rs1801177 was completely absent in the studied subjects. The SNPs were association with TG and HDL-C levels was checked through regression analysis. For rs328, the effect size of each risk allele on TG and HDL-C (mmol/l) was 0.16(0.08) and −0.11(0.05) respectively. Similarly, the effect size of rs662799 for TG and HDL-C was 0.12(0.06) and −0.13(0.0.3) and that of rs708272 was 0.08(0.04) and 0.1(0.03) respectively. The risk allele frequencies of the SNPs were higher in cases than controls, but the difference was not significant (p > 0.05) and SNPs were not associated with CAD risk (p > 0.05). The combined gene score of four SNPs significantly raised TG and lowered HDL-C but did not increase CAD risk.ConclusionThe studied SNPs were associated with TG and HDL-C levels, but not with CAD in Pakistani population under study.Electronic supplementary materialThe online version of this article (doi:10.1186/s12944-017-0419-4) contains supplementary material, which is available to authorized users.

Association of rs662799 variant and APOA5 gene haplotypes with metabolic syndrome and its components: a meta-analysis in North Africa

“…Our results showed the absence of a significant correlation between rs662799 and cardiovascular risk (data not shown). Our finding is in accordance with others previous studies reporting a lack of association between rs662799 and cardiac diseases in the Canadian population [43], Pakistani population [44], Costa Rican [21] and the United Kingdom population [45].…”

Sequence Analysis of APOA5 Among the Kuwaiti Population Identifies Association of rs2072560, rs2266788, and rs662799 With TG and VLDL Levels

“…In contrast, the usual association of SNPs rs662799, rs2072560, and rs2266788 minor alleles with elevated TG levels was detected amongst Kuwaiti Arabs ( p = 0.01, p = 0.04, p = 0.02, respectively). These findings are comparable with results found in other populations such as Mongolians (Chuluun-Erdene et al, 2017 ), Caucasians (Talmud et al, 2002 ), Uyghur population (Li et al, 2014 ) African Americans (Talmud et al, 2002 ; Lai et al, 2004 ), East Asians (Endo et al, 2002 ; Nabika et al, 2002 ), and Pakistani population (Shahid et al, 2017 ). The MAFs of both rs2072560 and rs2266788 in Kuwaiti Arab population was estimated to be 0.8, which resemble frequencies observed between African Americans and Caucasians (Pennacchio et al, 2002 ).…”

Relationship between CETP gene polymorphisms with coronary artery disease in Polish population

“…According to some studies, there is a relationship between some polymorphic variants of CETP and CAD [ 19 – 21 ]. However, other authors suggest a lack of association [ 22 – 24 ].…”