Common variable immunodeficiency classification by quantifying T-cell receptor and immunoglobulin κ-deleting recombination excision circles

Kamae, Chikako; Nakagawa, Noriko; Sato, Hiroki; Honma, Kenichi; Mitsuiki, Noriko; Ohara, Osamu; Kanegane, Hirokazu; Pasic, Srdjan; Pan‐Hammarström, Qiang; Zelm, Menno C. van; Morio, Tomohiro; Imai, Kohsuke; Nonoyama, Shigeaki

doi:10.1016/j.jaci.2012.10.059

Cited by 53 publications

(50 citation statements)

References 9 publications

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“…17 Thus, it is likely that younger donors have an advantage in terms of B-cell reconstitution. Our results indicated that a younger recipient age also contributed to increased levels of sjKRECs and cjKRECs.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Cord blood transplantation is associated with rapid B-cell neogenesis compared with BM transplantation

Nakatani

Imai

Shigeno

et al. 2014

Bone Marrow Transplant

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Hematopoietic cell transplantation (HCT) is used for treatment of hematopoietic diseases. Assessment of T-and B-cell reconstitution after HCT is crucial because poor immune recovery has a major effect on the clinical course. In this study, we retrospectively analyzed T-cell receptor excision circles (TRECs) as well as signal and coding joint kappa-deleting recombination excision circles (sjKRECs and cjKRECs, respectively) as markers of newly produced lymphocytes in 133 patients (56 primary immunodeficient and 77 malignant cases, median (range): 12 (0-62) years old). We analyzed the kinetics of TREC and KREC recovery and determined the factors that contributed to better immune recovery. KRECs became positive earlier than TRECs and increased thereafter. Younger recipient age had a favorable effect on recovery of sjKRECs and cjKRECs. Compared with BM and peripheral blood, our data suggested that cord blood (CB) provided rapid B-cell recovery. CB also provided better B-cell neogenesis in adult HCT recipients. Chronic GVHD was associated with low TRECs, but not increased sjKRECs/cjKRECs. Finally, positive sjKRECs 1 month after HCT were associated with fewer infectious episodes. Monitoring of TRECs and KRECs may serve as a useful tool for assessment of immune reconstitution post HCT.

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Section: Discussionmentioning

confidence: 99%

“…Measurement of TREC and KREC levels TREC, sjKREC and cjKREC levels were measured by real-time PCR as described previously [15][16][17][18][19] at 1, 3 and 6 months, and yearly after HCT. RNase P was used as an internal control.…”

Section: Introductionmentioning

confidence: 99%

Cord blood transplantation is associated with rapid B-cell neogenesis compared with BM transplantation

Nakatani

Imai

Shigeno

et al. 2014

Bone Marrow Transplant

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“…Kamae et al [33] indicated that low TREC and/or KREC levels are useful biomarkers that are associated with the overall survival rate in CVID patients. Moreover, TRECs and KRECs are positively correlated with absolute counts of naive T cells and naive B cells, respectively, as well as with memory B cells.…”

Section: T-cell Abnormalities In Cvidmentioning

confidence: 99%

T-Cell Abnormalities in Common Variable Immunodeficiency

Azizi

Rezaei

Kiaee

et al. 2016

J Investig Allergol Clin Immunol

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Common variable immunodeficiency (CVID) is the most common clinical primary immunodeficiency. It is characterized by a defect in B-cell differentiation to plasma and memory B cells. Moreover, numerous T-cell abnormalities have been reported and include decreased T-cell count and proliferative response, increased T-cell activation and apoptosis, and abnormalities in cytokine production. The aims of this review are to describe phenotypic and functional defects in T cells in CVID patients and to review the literature with respect to the effects of immunoglobulin replacement on the T-cell component in CVID patients. Key words: Common variable immunodeficiency. T cell. Helper T cells. Regulatory T cells. ResumenLa inmunodeficiencia común variable (CVID) es la inmunodeficiencia primaria más frecuente. Se caracteriza por un defecto en la diferenciación de linfocitos B hacia células plasmáticas y linfocitos B memoria. Se han descrito numerosas alteraciones en los linfocitos T de estos pacientes, tales como disminución en el número de linfocitos T y en sus respuestas proliferativas, aumento en la activación de células T y en la apoptosis, así como alteraciones en la producción de citokinas. El objetivo de esta revisión es describir las alteraciones funcionales y fenotípicas de los linfocitos T en los pacientes con CVID y revisar la bibliografía en relación a los efectos que la administración de inmunoglobulinas produce en los linfocitos T de los pacientes con CVID. Palabras clave: Inmunodeficiencia común variable. Células T. Células T helper. Células T reguladoras.

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“…In addition to B cell subset evaluation in CVID, quantitative defects (both increase and decrease in specific cell populations) in other lymphocyte subsets have also been reported in subsets of CVID patients, including T cells (99)(100)(101)(102)(103)(104) and NK cells (105). CVID patients with severe defects in naive T cell differentiation are now often classified as a separate entity, late-onset combined immunodeficiency (LOCID), as they likely represent another genetically distinct subset of CVID (106).…”

Section: Cellular Immunophenotyping In Pidsmentioning

confidence: 99%

Flow Cytometry, a Versatile Tool for Diagnosis and Monitoring of Primary Immunodeficiencies

Abraham

Aubert

2016

Clin Vaccine Immunol

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bGenetic defects of the immune system are referred to as primary immunodeficiencies (PIDs). These immunodeficiencies are clinically and immunologically heterogeneous and, therefore, pose a challenge not only for the clinician but also for the diagnostic immunologist. There are several methodological tools available for evaluation and monitoring of patients with PIDs, and of these tools, flow cytometry has gained prominence, both for phenotyping and functional assays. Flow cytometry allows real-time analysis of cellular composition, cell signaling, and other relevant immunological pathways, providing an accessible tool for rapid diagnostic and prognostic assessment. This minireview provides an overview of the use of flow cytometry in disease-specific diagnosis of PIDs, in addition to other broader applications, which include immune phenotyping and cellular functional measurements.

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Common variable immunodeficiency classification by quantifying T-cell receptor and immunoglobulin κ-deleting recombination excision circles

Cited by 53 publications

References 9 publications

Cord blood transplantation is associated with rapid B-cell neogenesis compared with BM transplantation

Cord blood transplantation is associated with rapid B-cell neogenesis compared with BM transplantation

T-Cell Abnormalities in Common Variable Immunodeficiency

Flow Cytometry, a Versatile Tool for Diagnosis and Monitoring of Primary Immunodeficiencies

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