Common pathogens isolated from burn wounds and their antibiotic resistance patterns

Yolbaş, İlyas; Tekin, Recep; Kelekçi, Selvi; Tayyar, C. Selçuk; Okur, Mesut; Tan, İlhan; Uluca, Ünal

doi:10.5798/diclemedj.0921.2013.03.0290

Cited by 8 publications

(8 citation statements)

References 14 publications

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“…The incidence of P. aeruginosa infection was higher in men than in females, 42 (56%) and 33 (44%) respectively. The results of the current study agree with the study of (15) of bacteria isolated from burn patients, which showed that the percentage of males was (77%) and females (23%), and also agrees with the study (Yolbaş et al, 2013), where This study showed infection rate for males (70%) and females (30%).…”

Section: 7supporting

confidence: 92%

Detection of some virulence factors of Pseudomonas aeruginosa as a result of treatment with antibiotic Levofloxacin and using specialist primer

2022

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The current study included a group of (150) clinical swabs for patients suffering from burns, infections in wounds, diabetic foot ulcers and urinary tract infections for both gender, (69) males and (81) females, their ages ranged between 1-68 years, for the period of November 2021 until the end of February 2022, from Hospitals in Mosul. After the final diagnosis of the samples, (75) isolates of P. aeruginosa were obtained, representing (50%) of the total clinical samples, and the rate of isolation of them from samples of burns, wounds, diabetic foot ulcers and urinary tract infections was 75% (42/56), 41.1% ( 23/56), 53.3% (8/15) and 8.7% (2/23), respectively.The antimicrobial susceptibility test was performed by the disk diffusion method, and it was found that the isolates are multi-antibiotic resistant. The percentage of resistance shown by the isolates were 70.7% for Piperacillin , 89.3% for Amikacin, 74.7% for Aztreonam and Levofloxacin , 81.3% for Gentamicin, Ceftazidime and Cefepime, 76% for Ciprofloxacin, 80% for Netilmicin and Tobramycin , and 45.3% for Imipenem.The effect of the antibiotic Levofloxacin on the PslA gene responsible for the production of biofilms, and the PhzM gene responsible for the production of the pyocyanin pigment by bacteria was studied by extracting the DNA of ten bacterial isolates and then performing a polymerase chain reaction (PCR) using the primers PslA, PhzM, and then performing the electrophoresis process For the final reaction products and to note the presence of Bands before and after the treatment of bacterial isolates with the antibiotic.Five isolates (PA1, PA4, PA5, PA6, PA7) were selected to perform DNA sequencing before and after treatment with the antibiotic Levofloxacin by sending the PCR products of the five samples with the 16SrRNA primer to the Macrogen biotechnology company to know the sequence of nitrogenous bases using a Genetic analyzer 3130 , Changes were observed in the sequence of nitrogenous bases after treatment with the antibiotic Levofloxacin, four new genes were recorded in the National Center for Biotechnology Information (NCBI), and these genes were named short for the name of the supervisor and researcher.

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Section: 7supporting

confidence: 92%

Detection of some virulence factors of Pseudomonas aeruginosa as a result of treatment with antibiotic Levofloxacin and using specialist primer

2022

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“…Results and Discussion The incidence of P. aeruginosa infection was higher in men than in females, 42 (56%) and 33 (44%) respectively. The results of the current study agree with the study of (AL-Shamaa et al, 2016) of bacteria isolated from burn patients, which showed that the percentage of males was (77%) and females (23%), and also agree with the study (Yolbaş et al, 2013), where This study showed infection rate for males (70%) and females (30%).…”

supporting

confidence: 93%

Effect of the antibiotic levofloxacin on the ability of Pseudomonas aeruginosa to form biofilm and produce pyocyanin pigment

2022

pnr

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The current study included a group of (150) clinical swabs for patients suffering from burns, wounds, diabetic ulcers, and urinary tract infections for (69) males and (81) females, their ages ranging from 1-68 years, for the period of November 2021 until March 2022, from Hospitals in Mosul. 75 samples of P. aeruginosa were isolated, representing (50%) of the total clinical samples, and the rate of isolation of them from samples of burns, wounds, diabetic foot ulcers, and urinary tract infections was 75% (42/56), 41.1% ( 23/56), 53.3% (8/15) and 8.7% (2/23), respectively. The antimicrobial susceptibility test was performed by the disk diffusion method, and it was found that the isolates are multi-antibiotic resistant. The percentage of resistance shown by the isolates was 70.7% for Piperacillin, 89.3% for Amikacin, 74.7% for Aztreonam and Levofloxacin, 81.3% for Gentamicin, Ceftazidime, and Cefepime, 76% for Ciprofloxacin, 80% for Netilmicin and Tobramycin, and 45.3% for Imipenem. The MIC values for the antibiotic Levofloxacin were determined using Macrodilution method, the results showed that the MIC value ranged between (0.5-512) µg/ml.The biofilm formation was assayed by the microtiter plate. Pyocyanin was extracted from the culture supernatants, and the absorbance values were measured using a spectrophotometer.The results showed that the antibiotic Levofloxacin at the MIC and Sub-MIC concentrations has an inhibitory effect to varying degrees on biofilm formation and pyocyanin pigment production.

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“…The findings were in accordance with various studies. 16,18 The percentage of colonization by Staphylococcus aureus did not show much change in the periodic samples. The persistence of Staphylococcus throughout our study could be due to cross-infection of micro-organisms in ICU.…”

Section: Discussionmentioning

confidence: 93%

“…15 Regarding growth of Acinetobacter baumanni, it was similar to other studies who also reported higher frequency of Acinetobacter infections. 16 Acinetobacter baumannii is a rapidly emerging pathogen in the health care setting, where it causes infections that include bacteremia, pneumonia, meningitis, urinary tract infection, and wound infection. The organism's ability to survive under a wide range of environmental conditions and to persist for extended periods of time on surfaces make it a frequent cause of outbreaks of infection and an endemic, health care-associated pathogen.…”

Section: Discussionmentioning

confidence: 99%

Time Related Changes in Pathogenic Bacterial Patterns in Burn Wound Infections and Their Antibiotic Sensitivity Traits

Mehta

Singh²,

Sawhney

et al. 2017

Bangladesh J Med Sci

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Objectives: To examine the time related changes in the bacterial flora isolated from the burn patients and to compare the antibiograms of the predominant bacteria. Burn injuries constitute a major health concern with respect to morbidity and mortality as well as cost of management particularly in developing countries. It has been estimated that 75% of all deaths following thermal injuries are related to infection. It is essential for every hospital to determine the specific pattern of burn wound microbial colonization, time-related changes in predominant flora and anti microbial sensitivity profiles. Materials and Methods: 60 burn patients and their microbial isolates were examined during the study period. Periodic swabs were taken from burn wounds of the patients on admission and on 7th, 14th, and 21st days of hospitalization and the isolates were processed for culture, identification and antimicrobial sensitivity as per CLSI guidelines. Results: Among the 240 samples, single organism was isolated in 161 samples (67.1%) and mixed organisms in 22 samples (9.1 %) and no growth in 57 samples (23.7%). Among single isolates pseudomonas aerugenosa was predominant species (20.8%) followed by acinetobacter baumanii (15.4%), staph. aureus (14.1%) and klebsiella (7.5%). Among mixed growth pseudomonas was predominant species (30.4%) followed by acinetobacter (23.9%). klebsiella (17.4 %) and staph. aureus (10.8%). There was time related changes in bacterial isolation from burn wound during hospital stay of patients. On admission, Staph. Aureus was 18.3 %, pseudomonas was 8.3% and klebsiella was 6.6%. No growth found in 48.3% samples. These findings gradually changed with time and on day 21 Pseudomonas was 30% followed by Acinetobacter sp. (21.6%), Staph. aureus (13.3%) and Klebsella pneumoniae (6.6%). No growth was seen in 15% cases only. Antimicrobial sensitivity test showed that pseudomonas was sensitive to Imipenem, Merepenem and Ampicillin. Acinetobacter was also found most sensitive to Amikacin and Tetracycline, followed by Tobramycin. Staphylococcus aureus was sensitive to Linezolid, Gentamycin followed by Vancomycin. Conclusion: There were timerelated changes in microbial colonization during hospital stay of patients. Effective protocols should be made for burn patients and effort is needed for improving overall infection related morbidity and mortality.

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Common pathogens isolated from burn wounds and their antibiotic resistance patterns

Cited by 8 publications

References 14 publications

Detection of some virulence factors of Pseudomonas aeruginosa as a result of treatment with antibiotic Levofloxacin and using specialist primer

Detection of some virulence factors of Pseudomonas aeruginosa as a result of treatment with antibiotic Levofloxacin and using specialist primer

Effect of the antibiotic levofloxacin on the ability of Pseudomonas aeruginosa to form biofilm and produce pyocyanin pigment

Time Related Changes in Pathogenic Bacterial Patterns in Burn Wound Infections and Their Antibiotic Sensitivity Traits

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