Common-path lensless digital holographic microscope employing a Fresnel biprism

Ebrahimi, Samira; Dashtdar, Masoomeh; Anand, Arun; Javidi, Bahram

doi:10.1016/j.optlaseng.2020.106014

Cited by 26 publications

(15 citation statements)

References 31 publications

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“…A major drawback in common-path DHM systems is their applicability to investigate quantitative phase images. The differences of that work 27 and our proposed FB-based system are the experimental resolution limit (

27 versus

) and the accuracy of the phase images using a single-shot approach. In Ref.…”

Section: Introductionmentioning

confidence: 87%

“…In Ref. 27, the sample is illuminated by two slightly tilted diverging spherical waves. Although both diverging spherical waves that illuminate the sample should have the same curvature, their origins (e.g., center of the spherical wave) are different, and therefore, they would never cancel each other.…”

Section: Introductionmentioning

confidence: 99%

“…In fact, note that the reconstructed phase image of the Candida albicans cells reported in Ref. 27 was generated after compensating the undesired spherical wavefront by a posteriori point-wise subtraction of the measured phase with no sample information placed from the measurement with the sample in place. The main drawback of this latter method is that it requires two shots, which may not always be possible in live cell imaging.…”

Section: Introductionmentioning

confidence: 99%

“… 26 In a close related work, Ebrahimi et al. 27 proposed an off-axis lensless DHM system using a FB. Because the system follows an off-axis configuration, meaning that there is no overlay between the different orders composing the Fourier spectrum of the recorded hologram, one can retrieve both amplitude and phase distributions by means of just spatial filtering the object frequencies from the spectrum of the single hologram.…”

Section: Introductionmentioning

confidence: 99%

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Advantages of Fresnel biprism-based digital holographic microscopy in quantitative phase imaging

et al. 2020

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Significance: The hallmarks of digital holographic microscopy (DHM) compared with other quantitative phase imaging (QPI) methods are high speed, accuracy, spatial resolution, temporal stability, and polarization-sensitivity (PS) capability. The above features make DHM suitable for real-time quantitative PS phase imaging in a broad number of biological applications aimed at understanding cell growth and dynamic changes occurring during physiological processes and/or in response to pharmaceutical agents. Aim: The insertion of a Fresnel biprism (FB) in the image space of a light microscope potentially turns any commercial system into a DHM system enabling QPI with the five desired features in QPI simultaneously: high temporal sensitivity, high speed, high accuracy, high spatial resolution, and PS. To the best of our knowledge, this is the first FB-based DHM system providing these five features all together. Approach: The performance of the proposed system was calibrated with a benchmark phase object. The PS capability has been verified by imaging human U87 glioblastoma cells. Results: The proposed FB-based DHM system provides accurate phase images with high spatial resolution. The temporal stability of our system is in the order of a few nanometers, enabling live-cell studies. Finally, the distinctive behavior of the cells at different polarization angles (e.g., PS capability) can be observed with our system. Conclusions: We have presented a method to turn any commercial light microscope with monochromatic illumination into a PS QPI system. The proposed system provides accurate quantitative PS phase images in a new, simple, compact, and cost-effective format, thanks to the low cost (a few hundred dollars) involved in implementing this simple architecture, enabling the use of this QPI technique accessible to most laboratories with standard light microscopes.

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27 versus

) and the accuracy of the phase images using a single-shot approach. In Ref.…”

Section: Introductionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Advantages of Fresnel biprism-based digital holographic microscopy in quantitative phase imaging

et al. 2020

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“…The loss of temporal stability can be overcome by incorporating common-path approaches, where interfering waves share identical or similar optical paths [ 28 , 29 , 30 ], thus compensating for environmental vibrations. In common-path configurations, increased attention has to be paid to designing a uniform reference wave, allowing for the primary classification of common-path approaches [ 31 , 32 , 33 , 34 , 35 ] as point diffraction or lateral shearing, which are appropriate for compact optical systems. The Shearing Device (SD) is incorporated in lateral shearing approaches, introducing the spatial carrier frequency and duplicating the FoV.…”

Section: Introductionmentioning

confidence: 99%

Developing a Reliable Holographic Flow Cyto-Tomography Apparatus by Optimizing the Experimental Layout and Computational Processing

Běhal

Borrelli²,

Mugnano

et al. 2022

Cells

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Digital Holographic Tomography (DHT) has recently been established as a means of retrieving the 3D refractive index mapping of single cells. To make DHT a viable system, it is necessary to develop a reliable and robust holographic apparatus in order that such technology can be utilized outside of specialized optics laboratories and operated in the in-flow modality. In this paper, we propose a quasi-common-path lateral-shearing holographic optical set-up to be used, for the first time, for DHT in a flow-cytometer modality. The proposed solution is able to withstand environmental vibrations that can severely affect the interference process. Furthermore, we have scaled down the system while ensuring that a full 360° rotation of the cells occurs in the field-of-view, in order to retrieve 3D phase-contrast tomograms of single cells flowing along a microfluidic channel. This was achieved by setting the camera sensor at 45° with respect to the microfluidic direction. Additional optimizations were made to the computational elements to ensure the reliable retrieval of 3D refractive index distributions by demonstrating an effective method of tomographic reconstruction, based on high-order total variation. The results were first demonstrated using realistic 3D numerical phantom cells to assess the performance of the proposed high-order total variation method in comparison with the gold-standard algorithm for tomographic reconstructions: namely, filtered back projection. Then, the proposed DHT system and the processing pipeline were experimentally validated for monocytes and mouse embryonic fibroblast NIH-3T3 cells lines. Moreover, the repeatability of these tomographic measurements was also investigated by recording the same cell multiple times and quantifying the ability to provide reliable and comparable tomographic reconstructions, as confirmed by a correlation coefficient greater than 95%. The reported results represent various steps forward in several key aspects of in-flow DHT, thus paving the way for its use in real-world applications.

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Investigation on the optical focusing effect of Fresnel biprism

Zhang

2023

Optoelectron. Lett.

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Common-path lensless digital holographic microscope employing a Fresnel biprism

Cited by 26 publications

References 31 publications

Advantages of Fresnel biprism-based digital holographic microscopy in quantitative phase imaging

Advantages of Fresnel biprism-based digital holographic microscopy in quantitative phase imaging

Developing a Reliable Holographic Flow Cyto-Tomography Apparatus by Optimizing the Experimental Layout and Computational Processing

Investigation on the optical focusing effect of Fresnel biprism

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