Common markers of testicular Sertoli cells

You, Xu; Chen, Qian; Yuan, Ding; Zhang, Changcheng; Zhao, Haixia

doi:10.1080/14737159.2021.1924060

Cited by 14 publications

(6 citation statements)

References 189 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Spermatogenic cells are supported by surrounding Sertoli cells, which produce the factors and microenvironment required for each stage of spermatogenic cell development ( Walker, 2021 ; You et al, 2021 ). The factors include GDNF, which promotes the proliferation and self-renewal of SSCs, and SCF, which encourages the differentiation of SSCs ( Guo et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

The Abscopal Effects of Cranial Irradiation Induce Testicular Damage in Mice

et al. 2021

View full text Add to dashboard Cite

To investigate whether the abscopal effects of cranial irradiation (C-irradiation) cause testicular damage in mice, male C57BL/6 mice (9weeks of age) were randomly divided into a sham irradiation group, a shielded group and a C-irradiation group and administered sham/shielded irradiation or C-irradiation at a dose rate of 2.33Gy/min (5Gy/d for 4 d consecutively). All mice were sacrificed at 4weeks after C-irradiation. We calculated the testis index, observed testicular histology by haematoxylin-eosin (HE) staining and observed testicular ultrastructure by transmission electron microscopy. Western blotting was used to determine the protein levels of Bax, Bcl-2, Cleaved caspase 3, glial cell line-derived neurotrophic factor (GDNF) and stem cell factor (SCF) in the testes of mice. Immunofluorescence staining was performed to detect the expression of Cleaved caspase 3 and 3β hydroxysteroid dehydrogenase (3βHSD), and a TUNEL assay was used to confirm the location of apoptotic cells. The levels of testosterone (T), GDNF and SCF were measured by ELISA. We also evaluated the sperm quality in the cauda epididymides by measuring the sperm count, abnormality, survival rate and apoptosis rate. The results showed that there was no significant difference in testicular histology, ultrastructure or sperm quality between the shielded group and sham group. Compared with the sham/shielded group, the C-irradiation group exhibited a lower testis index and severely damaged testicular histology and ultrastructure at 4weeks after C-irradiation. The levels of apoptosis in the testes increased markedly in the C-irradiation group, especially in spermatogonial stem cells. The levels of serum T and testicular 3βHSD did not obviously differ between the sham group and the C-irradiation group, but the levels of GDNF and SCF in the testes increased in the C-irradiation group, compared with the sham group. In addition, the sperm count and survival rate decreased in the C-irradiation group, while the abnormality and apoptosis rate increased. Under these experimental conditions, the abscopal effects of C-irradiation induced testicular damage with regard to both structure and function and ultimately decreased sperm quality in mice. These findings provide novel insights into prevention and treatment targets for male reproductive damage induced by C-irradiation.

show abstract

Section: Discussionmentioning

confidence: 99%

The Abscopal Effects of Cranial Irradiation Induce Testicular Damage in Mice

et al. 2021

View full text Add to dashboard Cite

show abstract

“…GATA4 is a transcription factor that can be used to identify SCs in testis ( 21 ). The fixed SCs were washed with PBS, treated with 0.1% triton-x (1 h) for permeabilization, and incubated with 2% bovine serum albumin (BSA) for 2 h to block protein non-specific binding, and the cells were incubated with primary antibody of rabbit anti-GATA4 (1:150 dilution, Bioss, China) and mouse anti-β-Tublin (1:150 dilution, Bioss, China) for 12 h at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Transcriptome sequencing reveals differences between leydig cells and sertoli cells of yak

Wang

Afedo

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

In this study, we detected the expression of mRNAs, lncRNAs, and miRNAs in primary cultured leydig cells (LCs) and sertoli cells (SCs) of yak by RNA sequencing technology. A total of 84 differently expression mRNAs (DEmRNAs) (LCs vs. SCs: 15 up and 69 down), 172 differently expression lncRNAs (DElncRNAs) (LCs vs. SCs: 36 up and 136 down), and 90 differently expression miRNAs (DEmiRNAs) (LCs vs. SCs: 72 up and 18 down) were obtained between the two types of cells. GO enrichment and KEGG analysis indicated that the differential expression genes (DEGs) were more enriched in the regulation of actin cytoskeleton, Rap1/MAPK signaling pathway, steroid biosynthesis, focal adhesion, and pathways associated with metabolism. Targeted regulation relationship pairs of 3β-HSD and MSTRG.54630.1, CNTLN and MSTRG.19058.1, BRCA2 and MSTRG.28299.4, CA2 and novel-miR-148, and ceRNA network of LAMC3-MSTRG.68870.1- bta-miR-7862/novel-miR-151/novel-miR-148 were constructed by Cytoscape software. In conclusion, the differences between LCs and SCs were mainly reflected in steroid hormone synthesis, cell proliferation and metabolism, and blood-testicular barrier (BTB) dynamic regulation, and 3β-HSD, CNTLN, BRCA2, CA2, and LAMC3 may be the key factors causing these differences, which may be regulated by ncRNAs. This study provides a basic direction for exploring the differential regulation of LCs and SCs by ncRNAs.

show abstract

“…The difficulty is that many adult Sertoli cell markers are also expressed in other cells in the body. 116 An alternative method to achieve Sertoli cell-specific alterations in adult animals is to use viral vectors. A few studies already used AAVs and lentiviral vectors to specifically transduce Sertoli cells in vivo.…”

Section: Challengesmentioning

confidence: 99%

What are Sertoli cells? Historical, methodological, and functional aspects

Thumfart

Mansuy

2023

Andrology

View full text Add to dashboard Cite

Sertoli cells are somatic cells that are in close contact with germ cells in the mammalian testes. They have multiple functions and fulfill key roles for the development and proper maturation of spermatogenic cells into functional spermatozoa. One of their most important properties is to release trophic factors and supply nutrients to germ cells. But they are also involved in the regulation of the immune system in testis, and provide an immunologically privileged environment for developing germ cells. Because they are so essential for reproductive cells, their alterations can have detrimental consequences for fertility. Many environmental factors and exposures such as high caloric diet, toxins, and pollutants are thought to compromise Sertoli cells physiology. This review describes the discovery of Sertoli cells and the methods used for their study, summarizes their major properties and functions, and describes their dysfunctions in pathologies, particularly associated with environmental stressors.

show abstract

Common markers of testicular Sertoli cells

Cited by 14 publications

References 189 publications

The Abscopal Effects of Cranial Irradiation Induce Testicular Damage in Mice

The Abscopal Effects of Cranial Irradiation Induce Testicular Damage in Mice

Transcriptome sequencing reveals differences between leydig cells and sertoli cells of yak

What are Sertoli cells? Historical, methodological, and functional aspects

Contact Info

Product

Resources

About