Common endothelial progenitor cell assays identify discrete endothelial progenitor cell populations

Povsic, Thomas J.; Zavodni, Katherine L.; Vainorius, Enrikas; Kherani, Jennifer; Goldschmidt‐Clermont, Pascal J.; Peterson, Eric D.

doi:10.1016/j.ahj.2008.10.010

Cited by 31 publications

(33 citation statements)

References 36 publications

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“…These markers are more reliable and accurate detectors of EPCs than other EPC culturebased assays (13). Interestingly, we also identified trends toward lower numbers of other exploratory markers of EPCs including cell surface expression of CD34…”

Section: Discussionmentioning

confidence: 61%

“…EPC number was assayed using flow cytometry on the basis of cell-surface expression of CD133, CD34, CD146, and CD14 as well as on the basis of aldehyde dehydrogenase (ALDH) activity, a property of stem cells, but lacking in more differentiated tissues, a method that is more precise than other EPC assays (12,13). Briefly, peripheral blood mononuclear cells (MNCs) were isolated using density gradient centrifugation and washed, and the relative level of EPCs in the MNC population was determined.…”

Section: Laboratory Proceduresmentioning

confidence: 99%

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Intradialytic Hypertension and its Association with Endothelial Cell Dysfunction

Inrig

Buren

Kim

et al. 2011

Clinical Journal of the American Society of Nephrology

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SummaryBackground and objectives Intradialytic hypertension is associated with adverse outcomes, yet the mechanism is uncertain. Patients with intradialytic hypertension exhibit imbalances in endothelial-derived vasoregulators nitric oxide and endothelin-1, indirectly suggesting endothelial cell dysfunction. We hypothesized that intradialytic hypertension is associated in vivo with endothelial cell dysfunction, a novel predictor of adverse cardiovascular outcomes. Design, settings, participants, & measurementsWe performed a case-control cohort study including 25 hemodialysis (HD) subjects without (controls) and 25 with intradialytic hypertension (an increase in systolic BP pre-to postdialysis Ն10 mmHg Ն4/6 consecutive HD sessions). The primary outcome was peripheral blood endothelial progenitor cells (EPCs) assessed by aldehyde dehydrogenase activity (ALDH br ) and cell surface marker expression (CD34 ϩ CD133 ϩ ). We also assessed endothelial function by ultrasonographic measurement of brachial artery flow-mediated vasodilation (FMD) normalized for shear stress. Parametric and nonparametric t tests were used to compare EPCs, FMD, and BP.Results Baseline characteristics and comorbidities were similar between groups. Compared with controls, 2-week average predialysis systolic BP was lower among subjects with intradialytic hypertension (144.0 versus 155.5 mmHg), but postdialysis systolic BP was significantly higher (159.0 versus 128.1 mmHg). Endothelial cell function was impaired among subjects with intradialytic hypertension as measured by decreased median ALDH br cells and decreased CD34 ϩ CD133 ϩ cells (ALDH br , 0.034% versus 0.053%; CD34 ϩ CD133 ϩ , 0.033% versus 0.059%). FMD was lower among subjects with intradialytic hypertension (1.03% versus 1.67%). ConclusionsIntradialytic hypertension is associated with endothelial cell dysfunction. We propose that endothelial cell dysfunction may partially explain the higher event rates observed in these patients.

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Section: Discussionmentioning

confidence: 61%

Section: Laboratory Proceduresmentioning

confidence: 99%

Intradialytic Hypertension and its Association with Endothelial Cell Dysfunction

Inrig

Buren

Kim

et al. 2011

Clinical Journal of the American Society of Nephrology

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“…ALDHbr cells from human bone marrow are highly enriched in progenitors that give rise to multipotent mesenchymal cells [25,26]. Endothelial progenitor cells are also highly enriched in human ALDHbr populations from bone marrow [25,26] and normal peripheral blood [35,36]. In the context of tissue repair, human ALDHbr cells, especially cells derived from bone marrow, can provide a mixture of potential progenitor and, as discussed below, paracrine activities from a variety of stem cell types.…”

Section: Aldhbr Cells From Sources Of Hematopoietic Graft In Transplamentioning

confidence: 99%

“…Povsic et al [35,36] reported that the number of ALDHbr cells circulating in the blood of cardiovascular patients was inversely correlated with the extent of coronary artery disease. This group suggested that circulating ALDHbr cell levels could be used to assess risk of cardiac disease and to monitor the efficacy of therapeutic interventions.…”

Section: Aldhbr Cell From Human Cb and Bone Marrow In Repair Of Ischementioning

confidence: 99%

Concise Review: Aldehyde Dehydrogenase Bright Stem and Progenitor Cell Populations from Normal Tissues: Characteristics, Activities, and Emerging Uses in Regenerative Medicine

Balber¹

2011

Stem Cells

102

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“…The EPC number was assayed using flow cytometry based on cell surface expression of CD34+CD133+ aldehyde dehydrogenase bright (ALDH br ) activity (16,17). Briefly, PBMCs were isolated using density gradient centrifugation and were washed, and relative level of EPCs in the mononuclear cell population was determined.…”

Section: Study Proceduresmentioning

confidence: 99%

Probing the Mechanisms of Intradialytic Hypertension

Inrig

Buren

Kim

et al. 2012

Clinical Journal of the American Society of Nephrology

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SummaryBackground and objectives Intradialytic hypertension may be caused by an impaired endothelial cell response to hemodialysis. Carvedilol has been shown to improve endothelial cell function in vivo and to block endothelin-1 release in vitro. This study hypothesized that carvedilol would improve endothelial cell function and reduce the occurrence of intradialytic hypertension.Design, setting, participants, & measurements A prospective 12-week pilot study of carvedilol titrated to 50 mg twice daily was performed among 25 hemodialysis participants with intradialytic hypertension. Each patient served as his or her own control. Paired tests were used to analyze changes in BP and endothelial cell functionassessed by flow-mediated vasodilation, endothelial progenitor cells (aldehyde dehydrogenase bright activity and CD34 + CD133 + ), asymmetric dimethylarginine, and endothelin-1-from baseline to study end.Results Flow-mediated vasodilation was significantly improved with carvedilol (from 1.03% to 1.40%, P=0.02). There was no significant change in endothelial progenitor cells, endothelin-1, or asymmetric dimethylarginine. Although prehemodialysis systolic BP was unchanged (144-146 mmHg, P=0.5), posthemodialysis systolic BP, 44-hour ambulatory systolic BP, and the frequency of intradialytic hypertension decreased with carvedilol (159-142 mmHg, P,0.001; 155-148 mmHg, P=0.05; and 77% [4.6 of 6] to 28% [1.7 of 6], P,0.001, respectively).Conclusions Among hemodialysis participants with intradialytic hypertension, targeting endothelial cell dysfunction with carvedilol was associated with modest improvements in endothelial function, improved intradialytic and interdialytic BP, and reduced frequency of intradialytic hypertension. Randomized controlled trials are required to confirm these findings.

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Common endothelial progenitor cell assays identify discrete endothelial progenitor cell populations

Abstract: Background-Multiple measures of endothelial progenitor cells (EPCs) have been described, but there has been limited study of the comparability of these assays. We sought to determine the reproducibility of and correlation between alternative EPC assay methodologies.

Cited by 31 publications

References 36 publications

Intradialytic Hypertension and its Association with Endothelial Cell Dysfunction

Intradialytic Hypertension and its Association with Endothelial Cell Dysfunction

Concise Review: Aldehyde Dehydrogenase Bright Stem and Progenitor Cell Populations from Normal Tissues: Characteristics, Activities, and Emerging Uses in Regenerative Medicine

Probing the Mechanisms of Intradialytic Hypertension

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