Common diseases of farm animals, by R. A. Craig... 123 illustrations in the text.

Craig, Robert Alexander

doi:10.5962/bhl.title.27345

Cited by 19 publications

(37 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is known that guinea-pig [34] (in contrast to rat [ 3 5 ] ) x-lactalbumin is unglycosylated. However, whilst it is clear that guinea-pig caseins A, B and C are glycosylated 1361. only casein C contains significant amounts of the neutral sugars [36]. It is not known whether these sugar residues are linked to asparagine NH2 residues (N-glycosylation) or serine OH or threonine OH residues (0-glycosylation) ; in other species oligosaccharide side-chains are exclusively 0-linked [37].…”

Section: Effect Of Tunicamycin On the Secretion Of Other Proteinsmentioning

confidence: 99%

The Influence of Topology and Glycosylation on the Fate of Heterologous Secretory Proteins Made in Xenopus Oocytes

Colman

Lane

Craig

et al. 1981

European Journal of Biochemistry

View full text Add to dashboard Cite

Secretory proteins made in Xenopus luevis oocytes under the direction of heterologous messenger RNA are modified, topologically segregated and exported. Thus the oocyte may serve as a useful surrogate secretory system and we have studied some of the factors governing access to the export pathway. Unglycosylated chicken ovalbumin, synthesized and trapped in the cytosol, is not secreted but glycosylated ovalbumin, found sequestered within vesicles, is exported from oocytes. However, ovalbumin, which is transferred across the endoplasmic reticulum in the presence of tunicamycin and which is indistinguishable by immunoprecipitation, by two-dimensional gel electrophoresis and by concanavalin-A -Sepharose binding from the cytosolic form, is still secreted. Guinea-pig milk proteins and human interferon are also exported from tunicamycin-treated frog cells. These observations demonstrate that access to the endoplasmic reticulum but not glycosylation is a mandatory intermediate step in secretion, and emphasize the advantages of the oocyte as a surrogate system for the study of the later events in the gene expression pathway.Oocytes of Xenopus luevis will export guinea-pig caseins and human interferon encoded by microinjected mRNAs; heterologous non-secretory proteins are not secreted [l]. Recently we have shown that secretory proteins from such diverse sources as rats, insects and plants are also selectively exported from oocytes (Lane et al., unpublished observations). These results demonstrate the possible usefulness of the oocyte as a general system for studies of protein secretion. In this paper we further examine the fidelity of protein secretion in this system by correlating the subcellular location and secondary modification of secretory proteins with their subsequent fate.Nascent polypeptide chains of secretory proteins contain a 'signal' sequence of some 15-30 amino acids [2-51, which interacts with a putative membrane receptor. This interaction results in the vectorial discharge of nascent polypeptides into the lumen of the endoplasmic reticulum. Secondary modification (e.g. glycosylation [6], phosphorylation [6]) of protein can occur within the endoplasmic reticulum and the use of tunicamycin, an inhibitor of glycosylation [7,8] indicates that this modification may protect proteins from degradation [9], affect the rate of secretion [lo, 111 or alter the intracellular destination of proteins [12]. The subsequent events in secretion are less well understood but are popularly thought Ahhreviution. PhMeSOZF, phenylmethylsulfonyl fluoride.to involve encapsulation of secretory polypeptides within the Golgi apparatus followed by movement of the resultant secretory vesicles to the cell surface, where exocytosis occurs [6,13,14].It is generally believed that the co-translational transfer of secretory proteins across the membranes of the endoplasmic reticulum is a mandatory step in the secretion of these proteins [3,4] ; the corollary of this theory is that proteins localized in the cytosol cannot be secreted. Much of...

show abstract

Section: Effect Of Tunicamycin On the Secretion Of Other Proteinsmentioning

confidence: 99%

The Influence of Topology and Glycosylation on the Fate of Heterologous Secretory Proteins Made in Xenopus Oocytes

Colman

Lane

Craig

et al. 1981

European Journal of Biochemistry

View full text Add to dashboard Cite

show abstract

“…In this case it gives a mixture of isomeric oximes 7a,b (anti-/syn-≈ 12 : 1, 1 H NMR data) through initial formation of plausible nitroso intermediate 6. The configuration of the C=N double bond in isomers 7a and 7b was established from 13 C NMR spectroscopic data, because it is known 27,28 that the oxime hydroxyl group exerts a substantial shielding effect on the adjacent α-carbon atom.…”

Section: Methodsmentioning

confidence: 99%

“…Optical rotation values were measured on a Jasco P-2000 polarimeter. 1 H and 13 C NMR spectra were recorded in CDCl3 or C6D6 at 303 K on a Bruker AM-300 spectrometer. Chemical shifts were reported relative to CHCl3 (δH = 7.27 and δC = 77.0) or C6H6 (δ H = 7.17 and δ C = 128.6).…”

Section: Methodsmentioning

confidence: 99%

“…Currently, there are few direct C-C bond formation reactions that have been successfully applied for the construction of all-carbon quaternary stereocenters. 1,9,10 Among them the enantioselective catalysis for allylic alkylation, 1,[9][10][11][12][13] conjugation addition, 14,15 Diels-Alder reactions 16 and some other reactions have been used. 17,18 There are significantly fewer examples of efficient control of the stereogenic process and stereochemistry of the newly formed quaternary center directed by chiral center or centers present initially in the starting materials.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enantiosective synthesis of a substituted cyclopentanone with all-carbon quaternary stereocenter

Lozanova¹,

Степанов²,

Zlokazov³

et al. 2017

Arkivoc

View full text Add to dashboard Cite

show abstract

“…Moreover, braceros eventually comprised one-third of the overall seasonal agricultural labour force in California (Pfeffer, 1980: 32;Calavita, 1992). Scholars have shown that the state managed migrants' mobility during this period of Mexico-US migration (Massey, 1986;Craig, 1971;Massey and Liang, 1989), but we know relatively little about how labour pools were organized in Mexico and who the key actors and institutions involved were. Although the bracero programme was operated by both the Mexican and US governments, our understanding about the programme focuses on how the US government and agribusiness regulated, instituted, and managed the programme (Pfeffer, 1980;Galarza, 1964;Calavita, 1992;Garcı´a y Griego, 1983).…”

Section: Introductionmentioning

confidence: 99%

The Sonoran Desert’s Domestic Bracero Programme: Institutional Actors and the Creation of Labour Migration Streams

Chávez

2012

International Migration

View full text Add to dashboard Cite

This paper traces the journeys of male migrants to Empalme, Sonora, Mexico to uncover the development of the often overlooked domestic bracero programme that operated in conjunction with its well-known international equivalent. Drawing on interviews and observations with ex-braceros who met at a park near the Mexico-US border, I examine their experiences and participation in Mexico's domestic bracero program, an unintended and unexplored consequence of its international counterpart. The study shows how regulation and control were constantly reinvented at every step of the selection process by state actors and their affiliates in Mexico. The paper reveals how the oversupply of labour and modernization of agriculture in Sonora resulted in the development of a migration industry where local municipal leaders, coyotes, the state, and Mexican agribusiness capitalized from men's displacement. The migration industry during the bracero selection process controlled who gained access to the United States labour market by capturing migrant labour en route to the United States in the process fueling a thriving cotton industry in the otherwise stagnant Sonoran Desert economy. The study concludes by taking the lessons from the historic domestic bracero programme to show one instance in which internal and international labour markets were closely interwoven. In the end, I call for more research that examines the relationship between markets on both sides of the border that uncovers how networks are not only structured by personal ties but also by state and market relations.

show abstract

Common diseases of farm animals, by R. A. Craig... 123 illustrations in the text.

Cited by 19 publications

References 0 publications

The Influence of Topology and Glycosylation on the Fate of Heterologous Secretory Proteins Made in Xenopus Oocytes

The Influence of Topology and Glycosylation on the Fate of Heterologous Secretory Proteins Made in Xenopus Oocytes

Enantiosective synthesis of a substituted cyclopentanone with all-carbon quaternary stereocenter

The Sonoran Desert’s Domestic Bracero Programme: Institutional Actors and the Creation of Labour Migration Streams

Contact Info

Product

Resources

About