Common Denominators of Promoter Control in Pseudomonas and Other Bacteria

Deretić, Vojo; Konyecsni, W M; Mohr, Christian; Martin, Daniel W.; Hibler, N S

doi:10.1038/nbt1289-1249

Cited by 75 publications

(82 citation statements)

References 46 publications

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“…The DNA sequence of IS1066 upstream of the putative start codon at position 184 further showed a possible ribosome binding site and promoter sequences (Fig. 2) (6). The end of IS1066 also contained a sequence homologous to the -35 promoter box TTGACA, facing outward (Fig.…”

Section: Resultsmentioning

confidence: 99%

Identification of a novel composite transposable element, Tn5280, carrying chlorobenzene dioxygenase genes of Pseudomonas sp. strain P51

1991

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Analysis of one of the regions of catabolic plasmid pP51 which encode chlorobenzene metabolism of Pseudomonas sp. strain P51 revealed that the tcbA and tcbB genes for chlorobenzene dioxygenase and dehydrogenase are located on a transposable element, Tn5280. Tn5280 showed the features of a composite bacterial transposon with iso-insertion elements (IS1066 and IS1067) at each end of the transposon oriented in an inverted position. When a 12-kb Hindlll fragment of pP51 containing Tn5280 was cloned in the suicide donor plasmid pSUP202, marked with a kanamycin resistance gene, and introduced into Pseudomonas putida KT2442, TnS280 was found to transpose into the genome at random and in single copy. The insertion elements IS1066 and IS1067 differed in a single base pair located in the inner inverted repeat and were found to be highly homologous to a class of repetitive elements of Bradyrhizobium japonicum and distantly related to IS630 of Shigella sonnei. The presence of the catabolic genes tcbA and tcbB on TnS280 suggests a mechanism by which gene clusters can be mobilized as gene cassettes and joined with others to form novel catabolic pathways. have been identified and characterized (49, 52) (Fig. 1). One region was found to contain a gene cluster, tcbCDEF, which encoded a modified ortho cleavage pathway (49, 52). The organization of the tcbCDEF gene cluster was found to be very similar to that of the tfdCDEF operon of Alcaligenes eutrophus JMP134 (8,14,34) and the clcABD operon of P. putida (11). The other region of pP51 contained the tcbAB gene cluster which encoded a chlorobenzene dioxygenase complex and a dehydrogenase, catalyzing the conversion of chlorobenzenes to chlorocatechols (52). This gene cluster exhibited size, organization, and function characteristics similar to those of other known bacterial dioxygenases, such as todCIC2BA from P. putida Fl (16,56), bphAB (12,23,30), or xylXYZ (20 respectively. E. coli S17-1 (41) was used as the donor strain for mobilization of pSUP202-derived plasmids. Pseudomonas putida KT2442 (hsdRl hsdM+) (10) was the recipient strain in mating experiments with E. coli S17-1 and was used to study transposition events. The strain is a rifampinresistant (Rif) plasmid-free derivative of strain P. putida mt-2. Plasmids pUC18 and pUC19 (55) were used as general

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Section: Resultsmentioning

confidence: 99%

Identification of a novel composite transposable element, Tn5280, carrying chlorobenzene dioxygenase genes of Pseudomonas sp. strain P51

1991

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“…Its broad range of biochemical abilities has been studied in detail, and its genome is currently being sequenced. P. aeruginosa promoter structure is quite varied [1,2], and many P. aeruginosa promoters are not well recognized in Escherichia coli. The identi¢-cation and characterization of promoter structures will therefore constitute a major challenge during interpretation of data emerging from the P. aeruginosa genome sequence project, and the results obtained will be doubly useful, since they will be paradigmatic for promoters throughout the large family of soil pseudomonads.…”

Section: Introductionmentioning

confidence: 99%

Construction of improved plasmid vectors for promoter characterization in Pseudomonas aeruginosa and other Gram-negative bacteria

Rist

1998

FEMS Microbiology Letters

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We report the construction of two broad host range promoter-probe plasmid vectors for rapid analysis of promoters in Gram-negative bacteria. The new vectors, pME4507 and pME4510, carry carbenicillin and gentamycin resistance genes, respectively, and are small sized (4 kb) with a flexible multiple cloning site to facilitate directional cloning of putative promoter elements. The vectors allow rapid plate-based screening for promoter activities, using L-galactosidase as the reporter enzyme. In the absence of an inserted promoter fragment, they display very low background activity, making them a useful tool for analysis of low expression level promoters. z

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“…However, no striking sequence homology or similarity has been observed between these proteins and eukaryotic histones. Recently, a Pseudomonas aeruginosa gene that encodes a protein resembling in its domanial organization and primary structure the eukaryotic Hi histones was described (10, 11,27) (Fig. 1).…”

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confidence: 99%

Immunocytochemical analysis of AlgP (Hp1), a histonelike element participating in control of mucoidy in Pseudomonas aeruginosa

1992

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AlgP, a protein with an unusual carboxy-terminal domain resembling the tails of eukaryotic Hi histones, was detected in whole-cell extracts and within the cells of Pseudomonas aeruginosa by using immunoblotting and immunoelectron microscopy analyses. One known function of AIgP is its participation in the transcriptional activation of the algD gene. This is a pivotal step in the establishment of mucoidy in P. aeruginosa; mucoidy is a critical virulence factor expressed during respiratory infections in patients with cystic fibrosis. Polyclonal and monoclonal antibodies were raised against a synthetic 50-mer peptide containing two sets of six tandem repeats of the motif Lys-Pro-Ala-Ala (and its single-amino-acid substitution variants), based on the sequence of the algP gene from the standard genetic strain PAO. Western immunoblots with these antibodies and total protein extracts from P. aeruginosa revealed two polypeptides that reacted with the antibodies in all of the P. aeruginosa strains tested. The detected polypeptides displayed strain-dependent variability in their electrophoretic mobility, in accordance with the previously noted variability of the algP repeats at the DNA level. In strain PAO, the recognized polypeptides had apparent masses of 46.4

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Common Denominators of Promoter Control in Pseudomonas and Other Bacteria

Cited by 75 publications

References 46 publications

Identification of a novel composite transposable element, Tn5280, carrying chlorobenzene dioxygenase genes of Pseudomonas sp. strain P51

Identification of a novel composite transposable element, Tn5280, carrying chlorobenzene dioxygenase genes of Pseudomonas sp. strain P51

Construction of improved plasmid vectors for promoter characterization in Pseudomonas aeruginosa and other Gram-negative bacteria

Immunocytochemical analysis of AlgP (Hp1), a histonelike element participating in control of mucoidy in Pseudomonas aeruginosa

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