Common Antigens of Streptococcal and Non-streptococcal Oral Bacteria: Isolation and Biochemical Characterization of the Extracellular Protein Antigen

Schöller, M; Klein, Jan Philipp; Sommer, Pascal; Frank, Robert M.

doi:10.1099/00221287-128-9-2113

Cited by 10 publications

(18 citation statements)

References 23 publications

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“…Extracellular LTA may be LTA which was originally associated with the cell walls before being released extracellularly in a manner similar to the extracellular glycoprotein antigen of some S. sanguis species. 29 Regarding the correlation between the human platelet aggregation and the presence of extracellular LTA, the fact that the LTA-containing culture supernates of a few test strains did not show any aggregation excludes the possibility that the platelet aggregation is due to extracellular LTA. Indeed, a report that the LTA of group A streptococci bound to human blood platelets but did not cause platelet aggregati~n,~~ suggests the opposite.…”

Section: Discussionmentioning

confidence: 99%

Biologically active extracellular products of oral viridans streptococci and the aetiology of Kawasaki disease

Ohkuni

Todome²,

Mizuse³

et al. 1993

Journal of Medical Microbiology

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Summary.A bacteriological study of isolates from the oral cavity of patients with Kawasaki disease (KD), age-matched non-KD patients and healthy children, showed that over half the KD and control isolates had gram-positive, catalase-negative cocci. About 50 % of these organisms were identified as viridans streptococci by means of an API Strep 20 kit. Further identification by fluorometric DNA-DNA hybridisation demonstrated that the predominant species were S. oralis and S. mitis, each of which accounted for 25 YO of the isolates of viridans streptococci ; 40 YO of viridans strains were unidentifiable ; and S. sanguis and S. parasanguis were minor components. Studies in vivo showed that insertion of culture supernates of most of the viridans streptococci increased capillary permeability and induced redness with swelling and occasional bleeding in rabbit skin. One-third of S. mitis strains and one-fifth of the unidentified strains caused aggregation of human blood platelets, whereas S. oralis and other strains had no such effect. The distribution of extracellular lipoteichoic acids and glucan produced in the presence of sucrose was also examined. There were no significant differences in the recovery rate of viridans streptococci forming these biologically active extracellular products between KD and control groups.

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Section: Discussionmentioning

confidence: 99%

Biologically active extracellular products of oral viridans streptococci and the aetiology of Kawasaki disease

Ohkuni

Todome²,

Mizuse³

et al. 1993

Journal of Medical Microbiology

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“…Glucosyltransferase activity is associated with components in the P2 and P3 regions (Hardy et al, 1986), and proteins whose molecular mass falls within the P3 range are a 74 kDa glucan-binding protein ) and a 60 kDa common antigen produced by a number of oral Gram-positive bacteria (Scholler et al;. Antigen B or 1/11 (Pl) has received considerable attention because of tests on its use in's caries vaccine (Cohen et al, 1983), its biological properties (Cohen et al, 1983;Ayakawa et al, 1985), and its potential involvement in adhesion (Douglas & Russell, 1984;McBride et al, 1985).…”

Section: K W Knox L N Hardy and A J Wicken Discussionmentioning

confidence: 99%

Comparative Studies on the Protein Profiles and Hydrophobicity of Strains of Streptococcus mutans Serotype c

Knox¹,

Hardy²,

Wicken³

1986

Microbiology

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Twelve strains of Streptococcus mutans serotype c were grown in batch culture with glucose at constant pH (6.0) and a number of properties compared. On the basis of their cellular and extracellular protein profiles, the strains were divided into three groups, I, I1 and 111, containing five, four and three strains, respectively. The extracellular protein profiles for a particular strain differed if the organisms were grown either at pH 6.0 with fructose instead of glucose or with glucose but without pH control. The total amount of extracellular protein produced by group I11 strains grown in glucose-containing medium at pH 6.0 was several times that produced by strains of groups I and 11, which were also more hydrophobic. One of the potentially important proteins is P1, also called antigen B or 1/11, and it was shown to be entirely in the culture fluid of group I11 strains but mostly cell-associated from strains of groups I and 11. Approximately half of the cell-associated fraction of P1 could be removed with hot sodium dodecyl sulphate.

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“…By the mid-1980's, it was known that many prominent plaque bacteria (those seen in developing plaque) shared many cross-reacting antigens (Scholler et al, 1981(Scholler et al, , 1983. Investigators proceeded to isolate, purify, and characterize these antigens and adhesins.…”

Section: Search For Microbial Adhesinsmentioning

confidence: 99%

“…Adhesins are also associated with Gram-negative outer cell membranes and/or vesicles. Families of adhesins among species which are nearly homologous genetically are reminiscent of the cross-reacting antigens found by Scholler et al (1983). For example, among the various viridans streptococci and within the sanguis streptococci are adhesins named FimA, ssab, ssp-5, SraA, Psia, and ScbA (Correiaetfl/., 1995).…”

Section: Search For Microbial Adhesinsmentioning

confidence: 99%

Human Oral Microbial Ecology and Dental Caries and Periodontal Diseases

Liljemark

Bloomquist

1996

Critical Reviews in Oral Biology & Medicine

221

149

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ABSTRACT:In the human oral cavity, which is an open growth system, bacteria must first adhere to a surface in order to be able to colonize. Ability to colonize a non-shedding tooth surface is necessary prior to any odontopathic or periodontopathic process. Complex microbe-host relationships occur and must be studied before the commensal-to-pathogenic nature of the human indigenous oral flora can be understood. Medical pathogens, if present in the appropriate host, always produce specific disease. Caries and periodontal diseases are conditional diseases, requiring numbers of certain indigenous species at various sites, particularly the tooth surface. In the case of caries, the condition is related to sugar consumption. Periodontal disease/s may require certain host and environmental conditions, such as local environment or nutritional factors in gingival crevicular fluids. Nonetheless, critical numbers of certain indigenous species must be present in order for these diseases to occur. The aim of this review is to understand the acquisition of the indigenous oral flora and the development of human dental plaque. The role of the salivary pellicle and adherence of indigenous bacteria to it are critical first steps in plaque development. Bacterial interactions with saliva, nutritional factors, growth factors, and microbial physiologic processes are all involved in the overall process of microbial colonization.

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Common Antigens of Streptococcal and Non-streptococcal Oral Bacteria: Isolation and Biochemical Characterization of the Extracellular Protein Antigen

Cited by 10 publications

References 23 publications

Biologically active extracellular products of oral viridans streptococci and the aetiology of Kawasaki disease

Biologically active extracellular products of oral viridans streptococci and the aetiology of Kawasaki disease

Comparative Studies on the Protein Profiles and Hydrophobicity of Strains of Streptococcus mutans Serotype c

Human Oral Microbial Ecology and Dental Caries and Periodontal Diseases

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