Common antigens of streptococcal and non-streptococcal oral bacteria: immunochemical studies of extracellular and cell-wall-associated antigens from Streptococcus sanguis, Streptococcus mutans, Lactobacillus salivarius, and Actinomyces viscosus

Schöller, M; Klein, Jan Philipp; Frank, Robert M.

doi:10.1128/iai.31.1.52-60.1981

Cited by 41 publications

(24 citation statements)

References 43 publications

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“…Investigation of the cell surface antigens of Streptococcus tnutans have identified 4 glycoproteins (SA, I, II, III and IV) in the culture supernatant of S. tnutatrs serotype c by immunodiffusion and immunoelectrophoresis against the corresponding antisera (17), Streptoeoeeal antigens (SA) I and II are usually associated in a single molecule, (SA I/II) of 185 kD whieh has been identified as a major cell surface antigen (14,16,26). Similar glycoproteins have been isolated by other workers (19,20). The SA determinants identified in the 185 kD molecule have been detected in smaller molecular sizes, down to a peptide of 3800 D (6).…”

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confidence: 74%

Characterisation of monoclonal antibodies to common protein epitopes on the cell surface of Streptococcus mutans and Streptococcus sobrinus

SMITH

Lehner

1989

Oral Microbiology and Immunology

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Three monoclonal antibodies (MAb) were prepared against a cell surface antigen which cross-react between Streptococcus mutans (serotypes c, e and f) and Streptococcus sobrinus (serotypes d and g). Two of the MAb also recognise a determinant on the surface of Streptococcus cricetus (serotype a). The common antigen shared between S. mutans and S. sobrinus was demonstrated by Western blotting to be about 200 kD in size. This antigen is shared not only by the cell surfaces of serotypes a, c, d, e, f and g, but also by the major cell surface antigen of S. mutans of 185 kD and another of 150 kD. These MAb identify all but one mutans type of streptococci and can be utilised as analytical reagents.

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confidence: 74%

Characterisation of monoclonal antibodies to common protein epitopes on the cell surface of Streptococcus mutans and Streptococcus sobrinus

SMITH

Lehner

1989

Oral Microbiology and Immunology

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“…While this contention cannot be completely excluded, Kilian (16) failed to observe any cross-reactivity between A. viscosus NY1, A. viscosus WVU 627 (A. naeslundii genospecies 2), and A. naeslundii ATCC 12104 (A. naeslundii genospecies 1) and Escherichia and Klebsiella typing sera. On the other hand, it has been reported (23,24) that A. viscosus OMZ104 (A. naeslundii genospecies 2), Streptococcus mutans, and Streptococcus sanguis have common wall-associated and extracellular protein antigens. However, S. sanguis was rarely isolated, and S. mutans was never isolated, from the mouths of the predentate infants in our study (21).…”

Section: Discussionmentioning

confidence: 99%

Humoral Immunity to Commensal Oral Bacteria in Human Infants: Salivary Antibodies Reactive with Actinomyces naeslundii Genospecies 1 and 2 during Colonization

Cole¹,

Bryan²,

Evans³

et al. 1998

Infect. Immun.

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The secretory immune response in saliva to colonization by Actinomyces naeslundii genospecies 1 and 2 was studied in 10 human infants from birth to 2 years of age. Actinomyces species were not recovered from the mouths of the infants until approximately 4 months after the eruption of teeth. However, low levels of secretory immunoglobulin A1 (SIgA1) and SIgA2 antibodies reactive with whole cells of A. naeslundii genospecies 1 and 2 were detected within the first month after birth. Although there was a fivefold increase in the concentration of SIgA between birth and age 2 years, there were no differences between the concentrations of SIgA1 and SIgA2 antibodies reactive with A. naeslundii genospecies 1 and 2 over this period. When the concentrations of SIgA1 and SIgA2 antibodies reactive with whole cells of A. naeslundii genospecies 1 and 2 were normalized to the concentrations of SIgA1 and SIgA2 in saliva, the A. naeslundii genospecies 1-and 2-reactive SIgA1 and SIgA2 antibodies showed a significant decrease from birth to 2 years of age. The fine specificities of A. naeslundii genospecies 1-and 2-reactive SIgA1 and SIgA2 antibodies were examined by Western blotting of envelope proteins. Similarities in the molecular masses of proteins recognized by SIgA1 and SIgA2 antibodies, both within and between subjects over time, were examined by cluster analysis and showed considerable variability. Taken overall, our data suggest that among the mechanisms Actinomyces species employ to persist in the oral cavity are the induction of a limited immune response and clonal replacement with strains differing in their antigen profiles.

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“…Bacteria were cultured at 37°C in brain–heart infusion broth (Difco Laboratories) with 1% glucose under anaerobic conditions. Cell wall extracted antigens were prepared from 18 hr culture as has been described 7, 14. Bacterial viability before and after extraction of surface proteins was unaffected.…”

Section: Methodsmentioning

confidence: 99%

Profiling the humoral immune response in colon cancer patients: Diagnostic antigens from Streptococcus bovis

Tjalsma

Schöller-Guinard

Lasonder

et al. 2006

Intl Journal of Cancer

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The human bowel contains a large and dynamic bacterial population that is not only essential for intestinal health, but also critical for the development of diseases such as cancer. In this respect, the Gram-positive bacterium Streptococcus bovis has been associated with colon cancer for many years. To investigate the clinical importance of this association, an immunocapture mass spectrometry assay was developed that can generate infection-related protein profiles. The composition of these profiles is governed by the capture of specific antigens by serum antibodies from colon cancer patients. This assay showed that S. bovis antigen profiles could distinguish 11 out of 12 colon cancer patients from 8 control subjects, whereas antigen profiles derived from the gut bacterium Escherichia coli were not diagnostic for colon cancer. Moreover, S. bovis antigen profiles were also detected in polyp patients, indicating that infection with this bacterium does occur early during carcinogenesis. Highly accurate tandem mass spectrometry was used to identify one of the diagnostic antigens as a surface-exposed heparin-binding protein, which might be involved in attachment of S. bovis to tumor cells. Together, these findings corroborate the hypothesis that colonic lesions provide a specific niche for S. bovis, resulting in tumor-associated ''silent'' infections. These infections, however, only become apparent in colon cancer patients with a compromised immune system (bacteremia) or coincidental cardiac valve lesions (endocarditis). This makes profiling of the humoral immune response against ''silent'' S. bovis infections a promising diagnostic tool for the early detection of human colon cancer, which is crucial for the effective treatment of this disease. ' 2006 Wiley-Liss, Inc. Key words: colon cancer; early detection; diagnostic bacterial antigens; Streptococcus bovisThe human bowel is the natural habitat for a large and dynamic bacterial community, which is essential for the control of intestinal epithelial homeostasis and human health.1,2 However, gut flora might also be an essential factor in certain diseases, including multisystem organ failure, inflammatory bowel diseases, and colon cancer.3 Although bacterial infections were originally not considered to be a major cause of cancer, accumulating evidence suggests that bacteria can induce or promote cancer by inflammation. In this model, tumor formation is caused or promoted by induction of cell proliferation and the production of mutagenic free radicals and n-nitroso compounds. In this respect, Helicobacter pylori has been the first invasive bacterium to be identified as a definite cause of gastric cancer. 4 Similarly, an association between Streptococcus bovis infection and colon cancer has been known for at least 25 years. Moreover, recent research has shown that S. bovis can promote intestinal carcinogenesis in a rat model for colon cancer.6 Certain cell surface proteins of this bacterium can induce inflammation, supporting a linkage between S. bovis, inflammation and ...

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Common antigens of streptococcal and non-streptococcal oral bacteria: immunochemical studies of extracellular and cell-wall-associated antigens from Streptococcus sanguis, Streptococcus mutans, Lactobacillus salivarius, and Actinomyces viscosus

Cited by 41 publications

References 43 publications

Characterisation of monoclonal antibodies to common protein epitopes on the cell surface of Streptococcus mutans and Streptococcus sobrinus

Characterisation of monoclonal antibodies to common protein epitopes on the cell surface of Streptococcus mutans and Streptococcus sobrinus

Humoral Immunity to Commensal Oral Bacteria in Human Infants: Salivary Antibodies Reactive with Actinomyces naeslundii Genospecies 1 and 2 during Colonization

Profiling the humoral immune response in colon cancer patients: Diagnostic antigens from Streptococcus bovis

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