Common and distinct factors regulate expression of mRNA for ETV5 and GDNF, Sertoli cell proteins essential for spermatogonial stem cell maintenance

Simon, Liz; Ekman, Gail C.; Tyagi, Gaurav; Hess, Rex A.; Murphy, Kenneth M.; Cooke, Paul S.

doi:10.1016/j.yexcr.2007.05.002

Cited by 83 publications

(83 citation statements)

References 56 publications

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“…Simon and colleagues recently confirmed this data by demonstrating that FSH increases the level of expression of Gdnf in the Sertoli cell line TM4 (Simon et al, 2007). In addition, Gdnf production by primary Sertoli cells is also dependent on Fgf2, tumor necrosis factor alpha (Tnfα) and interleukin-1beta (Il-1β) in vitro (Simon et al, 2007). Therefore, Gdnf production by Sertoli cells and SSCs self-renewal are controlled locally and systemically ( Figure 3).…”

Section: Spermatogenesis and The Spermatogonial Stem Cell Nichementioning

confidence: 82%

“…Conversely, primary cultures of Sertoli cells responded to FSH by an increase in Gdnf expression. Simon and colleagues recently confirmed this data by demonstrating that FSH increases the level of expression of Gdnf in the Sertoli cell line TM4 (Simon et al, 2007). In addition, Gdnf production by primary Sertoli cells is also dependent on Fgf2, tumor necrosis factor alpha (Tnfα) and interleukin-1beta (Il-1β) in vitro (Simon et al, 2007).…”

Section: Spermatogenesis and The Spermatogonial Stem Cell Nichementioning

confidence: 87%

“…As mentioned previously, Gdnf in the testis is produced by Sertoli cells, which establish the niche in which the SSCs reside (Viglietto et al, 2000;Meng et al, 2000;Meng et al, 2001a), and this activity extends throughout life (Chen et al, 2005). In Sertoli cells, the production of Gdnf is mainly under the influence of FSH, growth factors and cytokines (Tadokoro et al, 2002;Simon et al, 2007).…”

Section: Role Of Gdnf In the Mammalian Testismentioning

confidence: 94%

“…This phenotype provided the first evidence that a single transcription factor can regulate a stem cell niche in a vertebrate animal. Etv5 expression by Sertoli cells is up-regulated by Fgf2 and Egf, but interestingly, neither testosterone nor FSH seem to have any influence on this process, at least in vitro (Simon et al, 2007). Therefore, Etv5 expression might be regulated exclusively by local soluble factors (Figure 7).…”

Section: Etv5 and Other Transcription Factorsmentioning

confidence: 98%

“…The niche microenvironment is provided by the somatic Sertoli cells, the basement membrane and cellular components of the interstitial space between the seminiferous tubules. More specifically, these extrinsic signals include growth factors produced by Sertoli cells (Simon et al, 2007), adhesion molecules linking the SSCs to basement membrane components such as laminin (Orwig et al, 2002), and stimuli from the vascular network and interstitial cells (Yoshida et al, 2007). Spermatogonial stem cells are located at the periphery of the seminiferous epithelium, in contact with the basement membrane and Sertoli cells.…”

Section: Spermatogenesis and The Spermatogonial Stem Cell Nichementioning

confidence: 99%

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Gdnf signaling pathways within the mammalian spermatogonial stem cell niche

Hofmann

2008

Molecular and Cellular Endocrinology

200

177

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SummaryMammalian spermatogenesis is a complex process in which male germ-line stem cells develop to ultimately form spermatozoa. Spermatogonial stem cells, or SSCs, are found in the basal compartment of the seminiferous epithelium. They self-renew to maintain the pool of stem cells throughout life, or they differentiate to generate a large number of germ cells. A balance between SSC self-renewal and differentiation in the adult testis is therefore essential to maintain normal spermatogenesis and fertility. Maintenance and self-renewal are tightly regulated by extrinsic signals from the surrounding microenvironment, called the spermatogonial stem cell niche. By physically supporting the SSCs and providing them with growth factors, the Sertoli cell is the main component of the niche. In addition, adhesion molecules that connect the SSCs to the basement membrane and cellular components of the interstitium between the seminiferous tubules are important regulators of the niche function. This review mainly focuses on glial cell line-derived neurotrophic factor (Gdnf), which is produced by Sertoli cells to maintain SSCs self-renewal, and the downstream signaling pathways induced by this crucial growth factor. Interactions between Gdnf and other signaling pathways that maintain self-renewal, as well as the role of novel SSC-and Sertoli cell-specific transcription factors, are also discussed.

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Section: Spermatogenesis and The Spermatogonial Stem Cell Nichementioning

confidence: 82%

Section: Spermatogenesis and The Spermatogonial Stem Cell Nichementioning

confidence: 87%

Section: Role Of Gdnf In the Mammalian Testismentioning

confidence: 94%

Section: Etv5 and Other Transcription Factorsmentioning

confidence: 98%

Section: Spermatogenesis and The Spermatogonial Stem Cell Nichementioning

confidence: 99%

See 3 more Smart Citations

Gdnf signaling pathways within the mammalian spermatogonial stem cell niche

Hofmann

2008

Molecular and Cellular Endocrinology

200

177

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The enhancement of neural stem cell survival and growth by coculturing with expanded sertoli cells in vitro

Shi¹,

Deng²,

Shi³

et al. 2011

Biotechnology Progress

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Sertoli cells (SCs) have been described as the "nurse cells" of testis to provide essential growth factors and to create a proper environment for the development of other cells (e.g., germinal and neural stem cell). However, the physiological functions of the SCs obtained from different culture conditions are different in a coculturing system, and thus the optimal SC culturing condition should be investigated in vitro. In this paper, primary Sertoli cells were isolated from a 12-day-old mouse and expanded in two different culture conditions: a two dimensional (2D) plastic tissue disc and a three dimensional (3D) microcarrier culture system. They were then cocultured with neural stem cells (NSCs) isolated from 14-day-old mouse embryos. The metabolic activities of SCs(2D) (SCs in 2D) and SCs(3D) (SCs in 3D) and the amount of proteins secreted from two culturing systems were compared. The results show that the metabolic activity and the amount of secreted proteins from SCs(3D) were higher than both from SCs(2D). Three coculturing groups: NSCs+SC(2D), NSCs+SC(3D), and NSCs +SC-conditioned medium (SCCM, control group) were also compared regarding cell morphology and the numbers of neurons, neural outgrowths and neurospheres. The quantity of neurons, neural outgrowths and neurospheres were the highest in the NSCs+SC(3D) group. SCs cultured in the 3D system had a strong trophic effect on NSCs and enhanced their survival and growth. Besides, the mRNA of trophic and nutritive factors such as Glial-cell-line-derived neurotrophic factor (GDNF) and Interleukin-1 α (IL-1 α) secreted by the SCs from both 2D and 3D culture system were analyzed by real time-PCR and gel assay. The mRNA transcription of GDNF and IL-1α is more apparent in the 3D culture system than that from the 2D one. The coculturing system of NSCs+SC(3D) is a promising candidate for future neural stem cell transplantation.

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Porcine spermatogonial stem cells self‐renew effectively in a three dimensional culture microenvironment

Park

Kim

et al. 2017

Cell Biology International

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Generally, self-renewal of spermatogonial stem cells (SSCs) is maintained in vivo in a three-dimensional (3D) microenvironment consisting of the seminiferous tubule basement membrane, indicating the importance of the 3D microenvironment for in vitro culture of SSCs. Here, we report a 3D culture microenvironment that effectively maintains porcine SSC self-renewal during culture. Porcine SSCs were cultured in an agarose-based 3D hydrogel and in 2D culture plates either with or without feeder cells. Subsequently, the effects of 3D culture on the maintenance of undifferentiated SSCs were identified by analyzing cell colony formation and morphology, AP activity, and transcriptional and translational regulation of self-renewal-related genes and the effects on proliferation by analyzing cell viability and single cell-derived colony number. The 3D culture microenvironment constructed using a 0.2% (w/v) agarose-based 3D hydrogel showed the strongest maintenance of porcine SSC self-renewal and induced significant improvements in proliferation compared with 2D culture microenvironments. These results demonstrate that self-renewal of porcine SSCs can be maintained more effectively in a 3D than in a 2D culture microenvironment. Moreover, this will play a significant role in developing novel culture systems for SSCs derived from diverse species in the future, which will contribute to SSC-related research.

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Common and distinct factors regulate expression of mRNA for ETV5 and GDNF, Sertoli cell proteins essential for spermatogonial stem cell maintenance

Cited by 83 publications

References 56 publications

Gdnf signaling pathways within the mammalian spermatogonial stem cell niche

Gdnf signaling pathways within the mammalian spermatogonial stem cell niche

The enhancement of neural stem cell survival and growth by coculturing with expanded sertoli cells in vitro

Porcine spermatogonial stem cells self‐renew effectively in a three dimensional culture microenvironment

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