2009
DOI: 10.1016/j.devcel.2008.12.014
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Committed Neuronal Precursors Confer Astrocytic Potential on Residual Neural Precursor Cells

Abstract: During midgestation, mammalian neural precursor cells (NPCs) differentiate only into neurons. Generation of astrocytes is prevented at this stage, because astrocyte-specific gene promoters are methylated. How the subsequent switch from suppression to expression of astrocytic genes occurs is unknown. We show in this study that Notch ligands are expressed on committed neuronal precursors and young neurons in mid-gestational telencephalon, and that neighboring Notch-activated NPCs acquire the potential to become … Show more

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Cited by 322 publications
(348 citation statements)
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References 54 publications
(79 reference statements)
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“…NPCs were cultured in suspension as neurospheres in medium containing epidermal growth factor (EGF) and leukemia inhibitory factor (LIF) for 4-6 wk. In the absence of fibroblast growth factor 2 (FGF2), coapplication of EGF and LIF in prolonged culture promoted astroglial specification of NPCs (13,14) and efficiently selected for neurospheres with a high content of astroglial progenitors. After this enrichment phase, neurospheres were expanded in EGF-and FGF2-containing medium before enzymatic dissociation to single cells.…”
Section: Resultsmentioning
confidence: 99%
“…NPCs were cultured in suspension as neurospheres in medium containing epidermal growth factor (EGF) and leukemia inhibitory factor (LIF) for 4-6 wk. In the absence of fibroblast growth factor 2 (FGF2), coapplication of EGF and LIF in prolonged culture promoted astroglial specification of NPCs (13,14) and efficiently selected for neurospheres with a high content of astroglial progenitors. After this enrichment phase, neurospheres were expanded in EGF-and FGF2-containing medium before enzymatic dissociation to single cells.…”
Section: Resultsmentioning
confidence: 99%
“…In fact, two Drosophila Foxg1 orthologs, Sloppy paired-1 and -2 (Slp1 and Slp2), promote neuronogenesis at expenses of gliogenesis [59] and Foxg1 rescues the Slp1&2 null phenotype [60]. Such aberrant behavior of Foxg1-GOF NSCs might originate from unbalanced expression of Hes1, a key promoter of NSC self-renewal [61] and key gliogenesis promoters Coup-tf1, Nf1a, and Olig2 [62][63][64] (Supporting Information Fig. S11).…”
Section: Foxg1 Inhibits Gliogenesis and Potentiates Neuronogenesis Smentioning
confidence: 99%
“…L'activation de Notch résulte en l'induction du facteur de transcription NF1A (nuclear factor 1A), qui permet la déméthylation du promoteur du gène codant le marqueur astrocytaire GFAP (glial fibrillary acidic protein). Le promoteur ainsi déméthylé est activé dès que les cellules reçoivent un signal activateur dérivé de la voie Jak/Stat (Janus kinase/signal transducer and activator of transcription) [21]. Plusieurs mécanismes permettent d'éviter une production excessive d'astrocytes comme la répression de Hes1 par NF1A ou la dégradation du récepteur Notch1 par l'ubiquitine ligase Fbxw7 en fonction du stade de développement des CSN [22].…”
Section: Rôle De Notch Dans La Différenciation Neuronale Et L'équilibunclassified