Commitment of Annexin A2 in recruitment of microRNAs into extracellular vesicles

Hagiwara, Kenta; Katsuda, Takeshi; Gailhouste, Luc; Kosaka, Nobuyoshi; Ochiya, Takahiro

doi:10.1016/j.febslet.2015.11.036

Cited by 80 publications

(76 citation statements)

References 32 publications

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“…EVs isolated by discontinuous density gradient separation showed a heterogeneity in quantity and expression of the classical exosomal markers. The exosomal markers were mainly detected in the medium-density gradient fractions (1.08–1.14 g/mL density) which co-expressed the CD63 with classical mesenchymal vesicular markers such as CD29 and ITGA5 [2], and ANXA2 an important effector of miRNA recruitment in EVs [38]. …”

Section: Discussionmentioning

confidence: 99%

Exosome and Microvesicle-Enriched Fractions Isolated from Mesenchymal Stem Cells by Gradient Separation Showed Different Molecular Signatures and Functions on Renal Tubular Epithelial Cells

Collino

Pomatto²,

Bruno

et al. 2017

Stem Cell Rev and Rep

134

119

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Several studies have suggested that extracellular vesicles (EVs) released from mesenchymal stem cells (MSCs) may mediate MSC paracrine action on kidney regeneration. This activity has been, at least in part, ascribed to the transfer of proteins/transcription factors and different RNA species. Information on the RNA/protein content of different MSC EV subpopulations and the correlation with their biological activity is currently incomplete. The aim of this study was to evaluate the molecular composition and the functional properties on renal target cells of MSC EV sub-populations separated by gradient floatation. The results demonstrated heterogeneity in quantity and composition of MSC EVs. Two peaks of diameter were observed (90–110 and 170–190 nm). The distribution of exosomal markers and miRNAs evaluated in the twelve gradient fractions showed an enrichment in fractions with a flotation density of 1.08–1.14 g/mL. Based on this observation, we evaluated the biological activity on renal cell proliferation and apoptosis resistance of low (CF1), medium (CF2) and high (CF3) floatation density fractions. EVs derived from all fractions, were internalized by renal cells, CF1 and CF2 but not CF3 fraction stimulated significant cell proliferation. CF2 also inhibited apoptosis on renal tubular cells submitted to ischemia-reperfusion injury. Comparative miRNomic and proteomic profiles reveal a cluster of miRNAs and proteins common to all three fractions and an enrichment of selected molecules related to renal regeneration in CF2 fraction. In conclusion, the CF2 fraction enriched in exosomal markers was the most active on renal tubular cell proliferation and protection from apoptosis.Electronic supplementary materialThe online version of this article (doi:10.1007/s12015-016-9713-1) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Exosome and Microvesicle-Enriched Fractions Isolated from Mesenchymal Stem Cells by Gradient Separation Showed Different Molecular Signatures and Functions on Renal Tubular Epithelial Cells

Collino

Pomatto²,

Bruno

et al. 2017

Stem Cell Rev and Rep

134

119

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“…Annexin A2 exhibits sequence-specific RNA-binding activity and is involved in c-myc post-transcriptional regulation (Filipenko et al, 2004). Proteomic studies have revealed that Annexin A2 is among the most abundant proteins in EVs (Hagiwara et al, 2015). Hagiwara et al provided evidence that Annexin A2 can bind miRNA in the presence of Ca 2+ in diverse cancer cell lines (Hagiwara et al, 2015).…”

Section: Mechanisms Of Srna Sorting To Evsmentioning

confidence: 99%

“…Proteomic studies have revealed that Annexin A2 is among the most abundant proteins in EVs (Hagiwara et al, 2015). Hagiwara et al provided evidence that Annexin A2 can bind miRNA in the presence of Ca 2+ in diverse cancer cell lines (Hagiwara et al, 2015). The authors reported a global decrease in miRNA loading to cancer cell EVs upon Annexin A2 silencing.…”

Section: Mechanisms Of Srna Sorting To Evsmentioning

confidence: 99%

Small Luggage for a Long Journey: Transfer of Vesicle-Enclosed Small RNA in Interspecies Communication

Lefebvre

Lécuyer

2017

Front. Microbiol.

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In the evolutionary arms race, symbionts have evolved means to modulate each other's physiology, oftentimes through the dissemination of biological signals. Beyond small molecules and proteins, recent evidence shows that small RNA molecules are transferred between organisms and transmit functional RNA interference signals across biological species. However, the mechanisms through which specific RNAs involved in cross-species communication are sorted for secretion and protected from degradation in the environment remain largely enigmatic. Over the last decade, extracellular vesicles have emerged as prominent vehicles of biological signals. They can stabilize specific RNA transcripts in biological fluids and selectively deliver them to recipient cells. Here, we review examples of small RNA transfers between plants and bacterial, fungal, and animal symbionts. We also discuss the transmission of RNA interference signals from intestinal cells to populations of the gut microbiota, along with its roles in intestinal homeostasis. We suggest that extracellular vesicles may contribute to inter-species crosstalk mediated by small RNA. We review the mechanisms of RNA sorting to extracellular vesicles and evaluate their relevance in cross-species communication by discussing conservation, stability, stoichiometry, and co-occurrence of vesicles with alternative communication vehicles.

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“…Due to its known role in neurons, this protein may be of particular importance for microRNA export in the nervous system. Other proteins that have been implicated in the recruitment of microRNAs into EVs or in the selective export of microRNAs are oncogene KRAS [42], membrane-binding protein Annexin A2 [79], transcription factor KLF2 [80] and Rab family proteins [80]. However, their exact mechanisms are unknown.…”

Section: Microrna Loading Into Exosomesmentioning

confidence: 99%

Extracellular microRNAs as messengers in the central and peripheral nervous system

Scott

2017

Neuronal Signaling

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Correspondence : Hannah Scott (ScottH5@cardiff.ac.uk) MicroRNAs are small post-transcriptional regulators that play an important role in nervous system development, function and disease. More recently, microRNAs have been detected extracellularly and circulating in blood and other body fluids, where they are protected from degradation by encapsulation in vesicles, such as exosomes, or by association with proteins. These microRNAs are thought to be released from cells selectively through active processes and taken up by specific target cells within the same or in remote tissues where they are able to exert their repressive function. These characteristics make extracellular microRNAs ideal candidates for intercellular communication over short and long distances. This review aims to explore the potential mechanisms underlying microRNA communication within the nervous system and between the nervous system and other tissues. The suggested roles of extracellular microRNAs in the healthy and the diseased nervous system will be reviewed.

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Commitment of Annexin A2 in recruitment of microRNAs into extracellular vesicles

Cited by 80 publications

References 32 publications

Exosome and Microvesicle-Enriched Fractions Isolated from Mesenchymal Stem Cells by Gradient Separation Showed Different Molecular Signatures and Functions on Renal Tubular Epithelial Cells

Exosome and Microvesicle-Enriched Fractions Isolated from Mesenchymal Stem Cells by Gradient Separation Showed Different Molecular Signatures and Functions on Renal Tubular Epithelial Cells

Small Luggage for a Long Journey: Transfer of Vesicle-Enclosed Small RNA in Interspecies Communication

Extracellular microRNAs as messengers in the central and peripheral nervous system

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