Commercially produced frozen broccoli lacks the ability to form sulforaphane

Dosz, Edward B.; Jeffery, Elizabeth H.

doi:10.1016/j.jff.2013.01.033

Cited by 49 publications

(35 citation statements)

References 16 publications

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“…Freeze dried broccoli powder ( Brassica oleracea L. var. Green Magic), was kindly provided by Dr. John A. Juvik, which contained 4 mmol sulforaphane/kg broccoli powder, determined by hydrolysis of endogenous glucoraphanin, as previously reported (Dosz & Jeffery, 2013). Other diet ingredients were purchased from the Harlan Laboratory (Indianapolis, IN, USA).…”

Section: Methodsmentioning

confidence: 99%

Dietary broccoli protects against fatty liver development but not against progression of liver cancer in mice pretreated with diethylnitrosamine

Chen

Myracle

Wallig

et al. 2016

Journal of Functional Foods

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Western-style high fat, high sugar diets are associated with non-alcoholic fatty liver disease (NAFLD) and increased liver cancer risk. Sulforaphane from broccoli may protect against these. Previously we initiated broccoli feeding to mice prior to exposure to the hepatocarcinogen diethylnitrosamine (DEN), and saw protection against NAFLD and liver cancer. Here we administered DEN to unweaned mice, initiating broccoli feeding two weeks later, to determine if broccoli protects against cancer progression. Specifically, male 15-day-old C57BL/6J mice were given DEN and placed on a Western or Western+10%Broccoli diet from the age of 4 weeks through 7 months. Dietary broccoli decreased hepatic triacylglycerols, NAFLD, liver damage and tumour necrosis factor by month 5 without changing body weight or relative liver weight, but did not slow carcinogenesis, seen in 100% of mice. We conclude that broccoli, a good source of sulforaphane, slows progression of hepatic lipidosis, but not tumourigenesis in this robust model.

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Section: Methodsmentioning

confidence: 99%

Dietary broccoli protects against fatty liver development but not against progression of liver cancer in mice pretreated with diethylnitrosamine

Chen

Myracle

Wallig

et al. 2016

Journal of Functional Foods

Self Cite

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“…Multiple commercially developed sulforaphane supplements are currently available; however, the difficulty in manufacturing a potent and bioavailable formula has proved to be difficult, with an intrinsic instability of the sulforaphane molecule preventing this method of delivery (64). Manufacturing a sulforaphane-yielding supplement requires the ability to retain both the glucoraphanin precursor and the myrosinase enzyme for subsequent metabolism and transformation to the bioactive isothiocyanate (111).…”

Section: Sulforaphane In Human Clinical Trialsmentioning

confidence: 99%

Dietary Sulforaphane in Cancer Chemoprevention: The Role of Epigenetic Regulation and HDAC Inhibition

Tortorella

Royce²,

Licciardi

et al. 2015

Antioxidants & Redox Signaling

182

120

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Significance: Sulforaphane, produced by the hydrolytic conversion of glucoraphanin after ingestion of cruciferous vegetables, particularly broccoli and broccoli sprouts, has been extensively studied due to its apparent health-promoting properties in disease and limited toxicity in normal tissue. Recent Studies: Recent identification of a sub-population of tumor cells with stem cell-like self-renewal capacity that may be responsible for relapse, metastasis, and resistance, as a potential target of the dietary compound, may be an important aspect of sulforaphane chemoprevention. Evidence also suggests that sulforaphane may target the epigenetic alterations observed in specific cancers, reversing aberrant changes in gene transcription through mechanisms of histone deacetylase inhibition, global demethylation, and microRNA modulation. Critical Issues: In this review, we discuss the biochemical and biological properties of sulforaphane with a particular emphasis on the anticancer properties of the dietary compound. Sulforaphane possesses the capacity to intervene in multistage carcinogenesis through the modulation and/or regulation of important cellular mechanisms. The inhibition of phase I enzymes that are responsible for the activation of pro-carcinogens, and the induction of phase II enzymes that are critical in mutagen elimination are well-characterized chemopreventive properties. Furthermore, sulforaphane mediates a number of anticancer pathways, including the activation of apoptosis, induction of cell cycle arrest, and inhibition of NFjB. Future Directions: Further characterization of the chemopreventive properties of sulforaphane and its capacity to be selectively toxic to malignant cells are warranted to potentially establish the clinical utility of the dietary compound as an anticancer compound alone, and in combination with clinically relevant therapeutic and management strategies.

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“…Thus boiling for more than a minute, or steaming for more than 4 or 5 min will lead to loss of myrosinase activity (Wang, Farnham, & Jeffery, 2012). Furthermore, typically frozen products are blanched prior to freezing, which can be sufficient to destroy myrosinase activity (Dosz & Jeffery, 2013;Saha et al, 2012). Exogenous myrosinase is able to hydrolyze broccoli GSL.…”

Section: Glucoraphaninmentioning

confidence: 99%

Glucosinolate hydrolysis and bioavailability of resulting isothiocyanates: Focus on glucoraphanin

Angelino

Jeffery

2014

Journal of Functional Foods

128

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Commercially produced frozen broccoli lacks the ability to form sulforaphane

Cited by 49 publications

References 16 publications

Dietary broccoli protects against fatty liver development but not against progression of liver cancer in mice pretreated with diethylnitrosamine

Dietary broccoli protects against fatty liver development but not against progression of liver cancer in mice pretreated with diethylnitrosamine

Dietary Sulforaphane in Cancer Chemoprevention: The Role of Epigenetic Regulation and HDAC Inhibition

Glucosinolate hydrolysis and bioavailability of resulting isothiocyanates: Focus on glucoraphanin

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