Commercially available chemicals as immunizing haptens for the development of a polyclonal antibody recognizing carbendazim and other benzimidazole-type fungicides

Zikos, Christos; Evangelou, Alexandra; Karachaliou, Chrysoula-Evangelia; Gourma, Georgia; Blouchos, Petros; Moschopoulou, Georgia; Yialouris, Constantinos P; Griffiths, John; Johnson, Graham; Petrou, Panagiota; Kakabakos, Sotirios; Kintzios, Spyridon; Livaniou, Evangelia

doi:10.1016/j.chemosphere.2014.03.049

Cited by 22 publications

(25 citation statements)

References 23 publications

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“…The use of synthetic fungicides is the most common method of postharvest disease control (Zikos et al. ); however, for many fruit for export, including avocado, the application of fungicides is not an option. Prusky and Kobiler () mentioned that ‘new, strict regulations regarding fungicide residues, and the reductions by local authorities of the legal acceptability limits of specific fungicides make the development of new postharvest decay‐control treatments for fruits an important factor in improving their quality, and their safety for humans and the environment'.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Nanostructured Chitosan and Chitosan‐thyme Essential Oil Coatings on Colletotrichum gloeosporioides Growth in vitro and on cv Hass Avocado and Fruit Quality

Correa‐Pacheco

Bautista‐Baños

Valle-Marquina³

et al. 2017

Journal of Phytopathology

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In this study, nanostructured edible coatings based on chitosan nanoparticles (CSNPs) and chitosan‐thyme essential oil nanoparticles (CSTEO‐NPs) were characterized and evaluated on in vitro growth of Colletotrichum gloeosporioides and on inoculated avocado fruit cv. Hass, to evaluate their fungicidal activity and the effect on fruit quality. From TEM and particle size distribution characterization, the size of nanoparticles increased after thyme essential oil incorporation. Overall a synergistic effect between the chitosan and thyme essential oil (TEO) was observed. For in vitro evaluation, incorporation of this essential oil to CSNPs improved the control of C. gloeosporioides as there was a complete growth inhibition. CSNPs with concentrations of TEO at 3 and 5% had a fungicidal effect. The coating formulation with 55% CSTEO‐NPs notably reduced the incidence of C. gloeosporioides on avocado cv. Hass by up to 60%. Also, at the end of the 8‐day storage period, CSTEO‐NPs incorporation into the coating did not affect the quality of avocado; moreover, fruit firmness was better maintained than untreated fruit.

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Section: Introductionmentioning

confidence: 99%

The Effect of Nanostructured Chitosan and Chitosan‐thyme Essential Oil Coatings on Colletotrichum gloeosporioides Growth in vitro and on cv Hass Avocado and Fruit Quality

Correa‐Pacheco

Bautista‐Baños

Valle-Marquina³

et al. 2017

Journal of Phytopathology

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“…The top 12 drug-like compounds populate five distinct classes ( Figure 6 ). The benzimidazole-containing compound carbendazim is a well-known fungicide widely used in agricultural production ( Zikos et al, 2015 ). In addition, recently the benzimidazole class of compounds have been shown to inhibit a G+ bacterium, Staphylococcus aureus by targeting DNA gyraseB enzyme ( Janupally et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Discovery of novel small molecule modulators of Clavibacter michiganensis subsp. michiganensis

et al. 2015

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Clavibacter michiganensis subsp. michiganensis (Cmm) is a Gram-positive seed-transmitted bacterial phytopathogen responsible for substantial economic losses by adversely affecting tomato production worldwide. A high-throughput, cell-based screen was adapted to identify novel small molecule growth inhibitors to serve as leads for future bactericide development. A library of 4,182 compounds known to be bioactive against Saccharomyces cerevisiae was selected for primary screening against Cmm wild-type strain C290 for whole-cell growth inhibition. Four hundred sixty-eight molecules (11.2% hit rate) were identified as bacteriocidal or bacteriostatic against Cmm at 200 μM. Seventy-seven candidates were selected based on Golden Triangle analyses for secondary screening. Secondary screens showed that several of these candidates were strain-selective. Several compounds were inhibitory to multiple Cmm strains as well as Bacillus subtilis, but not to Pseudomonas fluorescens, Mitsuaria sp., Lysobacter enzymogenes, Lactobacillus rhamnosus, Bifidobacterium animalis, or Escherichia coli. Most of the compounds were not phytotoxic and did not show overt host toxicity. Using a novel 96-well bioluminescent Cmm seedling infection assay, we assessed effects of selected compounds on pathogen infection. The 12 most potent novel molecules were identified by compiling the scores from all secondary screens combined with the reduction of pathogen infection in planta. When tested for ability to develop resistance to the top-12 compounds, no resistant Cmm were recovered, suggesting that the discovered compounds are unlikely to induce resistance. In conclusion, here we report top-12 compounds that provide chemical scaffolds for future Cmm-specific bactericide development.

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“…A broadly specific polyclonal antibody showed selectivity to eleven organothiophosphate pesticides and five organophosphate pesticides [39]. Using the second approach, three 2-(2-aminoethyl) benzimidazole, 2-benzimidazole propionic acid, and 2-mercaptobenzimidazole have been conjugated to keyhole limpet hemocyanin, and the resulting mixture was used as the immunogen [40]. The obtained antibody could recognize carbendazim and other benzimidazole-type fungicides.…”

Section: Preparation and Application Of Broad-spectrum Antibodiesmentioning

confidence: 99%

Rapid Multi-Residue Detection Methods for Pesticides and Veterinary Drugs

et al. 2020

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The excessive use or abuse of pesticides and veterinary drugs leads to residues in food, which can threaten human health. Therefore, there is an extremely urgent need for multi-analyte analysis techniques for the detection of pesticide and veterinary drug residues, which can be applied as screening techniques for food safety monitoring and detection. Recent developments related to rapid multi-residue detection methods for pesticide and veterinary drug residues are reviewed herein. Methods based on different recognition elements or the inherent characteristics of pesticides and veterinary drugs are described in detail. The preparation and application of three broadly specific recognition elements—antibodies, aptamers, and molecular imprinted polymers—are summarized. Furthermore, enzymatic inhibition-based sensors, near-infrared spectroscopy, and SERS spectroscopy based on the inherent characteristics are also discussed. The aim of this review is to provide a useful reference for the further development of rapid multi-analyte analysis of pesticide and veterinary drug residues.

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Commercially available chemicals as immunizing haptens for the development of a polyclonal antibody recognizing carbendazim and other benzimidazole-type fungicides

Cited by 22 publications

References 23 publications

The Effect of Nanostructured Chitosan and Chitosan‐thyme Essential Oil Coatings on Colletotrichum gloeosporioides Growth in vitro and on cv Hass Avocado and Fruit Quality

The Effect of Nanostructured Chitosan and Chitosan‐thyme Essential Oil Coatings on Colletotrichum gloeosporioides Growth in vitro and on cv Hass Avocado and Fruit Quality

Discovery of novel small molecule modulators of Clavibacter michiganensis subsp. michiganensis

Rapid Multi-Residue Detection Methods for Pesticides and Veterinary Drugs

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