Commercial naphthenic acids and the organic fraction of oil sands process water downregulate pro-inflammatory gene expression and macrophage antimicrobial responses

García‐García, Erick; Pun, Jonathan; Pérez-Estrada, Leónidas A.; Din, Mohamed Gamal-El; Smith, Daniel; Martin, Jonathan W.; Belosevic, Miodrag

doi:10.1016/j.toxlet.2011.03.005

Cited by 60 publications

(47 citation statements)

References 68 publications

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“…The analysis of the presence and concentration of other substances in extracts could be beneficial for better understanding of their toxicological properties, but it is worth noting that the toxicological interactions between the compounds should also be taken into account. Garcia-Garcia et al (2011b) found that the inhibitory effect of OSPW organic fraction on BMDM NO production was eliminated by ozonation, while in this current study, increased immunotoxicity was observed in goldfish macrophages exposed to ozonated extracts. NAs were degraded by ozonation, regardless of their size or complexity (i.e., n and Z values, respectively), by 37% to 91% for the four extracts currently.…”

Section: In Vitro Toxicity Assessment Using Goldfish Macrophage Antimcontrasting

confidence: 47%

“…Fish were acclimated for at least 3 weeks prior to using them in experiments and euthanized by using tricaine methane sulfonate (TMS). Primary kidney macrophages (PKMs) were isolated and cultivated in complete NMGFL-15 medium, supplemented with 10% newborn calf and 5% carp serum as described previously (Garcia-Garcia et al, 2011b). Seven-day old PKMs were harvested, enumerated and used for nitric oxide assay.…”

Section: Fischeri Toxicity Bioassays and Goldfish Cell Nitric Oxidmentioning

confidence: 99%

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Effect of ozonation on the naphthenic acids' speciation and toxicity of pH-dependent organic extracts of oil sands process-affected water

Klamerth

Moreira

et al. 2015

Science of The Total Environment

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Section: In Vitro Toxicity Assessment Using Goldfish Macrophage Antimcontrasting

confidence: 47%

Section: Fischeri Toxicity Bioassays and Goldfish Cell Nitric Oxidmentioning

confidence: 99%

Effect of ozonation on the naphthenic acids' speciation and toxicity of pH-dependent organic extracts of oil sands process-affected water

Klamerth

Moreira

et al. 2015

Science of The Total Environment

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“…Studies involving mammals have been conducted and allow for the potential impacts of OSPW on reclaimed landscapes to be considered (Garcia-Garcia, Pun et al 2011;Garcia-Garcia et al 2012;Fu et al 2017). Additionally, mammalian studies that involve exposure to OSPW focusing on liver and immune responses as indicators of metabolism and excretion have relevance to, and can inform, human health implications (Rogers et al 2002;Garcia-Garcia, Ge et al 2011;Garcia-Garcia et al 2012).…”

Section: Workhop Highlightsmentioning

confidence: 99%

Overview of Existing Science to Inform Oil Sands Process Water Release: A Technical Workshop Summary

Tanna¹,

Redman

Frank

et al. 2019

Integr Envir Assess & Manag

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The extraction of oil sands from mining operations in the Athabasca Oil Sands Region uses an alkaline hot water extraction process. The oil sands process water (OSPW) is recycled to facilitate material transport (e.g., ore and tailings), process cooling, and is also reused in the extraction process. The industry has expanded since commercial mining began in 1967 and companies have been accumulating increasing inventories of OSPW. Short‐ and long‐term sustainable water management practices require the ability to return treated water to the environment. The safe release of OSPW needs to be based on sound science and engineering practices to ensure downstream protection of ecological and human health. A significant body of research has contributed to the understanding of the chemistry and toxicity of OSPW. A multistakeholder science workshop was held in September 2017 to summarize the state of science on the toxicity and chemistry of OSPW. The goal of the workshop was to review completed research in the areas of toxicology, chemical analysis, and monitoring to support the release of treated oil sands water. A key outcome from the workshop was identifying research needs to inform future water management practices required to support OSPW return. Another key outcome of the workshop was the recognition that methods are sufficiently developed to characterize chemical and toxicological characteristics of OSPW to address and close knowledge gaps. Industry, government, and local indigenous stakeholders have proceeded to utilize these insights in reviewing policy and regulations. Integr Environ Assess Manag 2019;15:519–527. © 2019 SETAC

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“…Their impacts include decreasing genetic diversity in wetland microbial consortia (Hadwin et al 2006), growth impacts in emergent macrophytes (Armstrong et al 2008), endocrine disruption in fish (Kavanagh et al 2012, Knag et al 2013, inducing abnormal embryonic development in yellow perch (Perca flavescens) and Japanese medaka (Orizias latipes; Peters et al 2007), and hepatic and developmental effects in Lithobates pipiens tadpoles (Melvin et al 2013). However, recent studies demonstrate differences in the toxicity of NAFC mixtures in comparison to model NAs (Peters et al 2007, Armstrong et al 2008, Garcia-Garcia et al 2011.…”

Section: Oil-sands Extraction and Development In The Fortmentioning

confidence: 99%

Evaluation of biologically mediated changes in oil sands naphthenic acid composition by Chlamydomonas reinhardtii using negative‐ion electrospray orbitrap mass spectrometry

Goff

Peru

Wilson

et al. 2014

Journal of Phycology

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Industrial activity associated with oil-sands extraction in Canada's Athabasca region produces a variety of contaminants of concern, including naphthenic acid fraction components (NAFCs). NAFCs are a complex mixture of organic compounds that are poorly understood both in terms of their chemical composition and effects on the environment. NAFC toxicity in the unicellular green algae Chlamydomonas reinhardtii P.A.Dangeard was correlated with the presence of the algal cell wall. It was suggested that the toxicity of NAFCs in C. reinhardtii was due to surfactant effects. Surfactant-cell wall interactions are specific and governed by the compound class and structure, and by the nature of the biological material. Here, we investigate the effects of wildtype (WT) C. reinhardtii and two cell-wall mutants on specific classes of NAFCs when growing cultures were treated with a 100 mg · L(-1) solution of NAFCs. Changes in the NAFC composition in the media were examined using high resolution mass spectrometry over a period of 4 d. Algal mediated changes in the NAFCs were limited to specific classes of NAFCs. In particular, the removal of large, classical naphthenic acids, with a double bond equivalent of 8, was observed in WT C. reinhardtii cultures. The observed algal mediated changes in NAFC composition would have been masked by low resolution mass spectrometry and highlight the importance of this tool in examining bioremediation of complex mixtures of NAFCs.

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Commercial naphthenic acids and the organic fraction of oil sands process water downregulate pro-inflammatory gene expression and macrophage antimicrobial responses

Cited by 60 publications

References 68 publications

Effect of ozonation on the naphthenic acids' speciation and toxicity of pH-dependent organic extracts of oil sands process-affected water

Effect of ozonation on the naphthenic acids' speciation and toxicity of pH-dependent organic extracts of oil sands process-affected water

Overview of Existing Science to Inform Oil Sands Process Water Release: A Technical Workshop Summary

Evaluation of biologically mediated changes in oil sands naphthenic acid composition by Chlamydomonas reinhardtii using negative‐ion electrospray orbitrap mass spectrometry

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