Commercial Micropropagation

Kitto, S.L.

doi:10.21273/hortsci.32.6.1012

Cited by 25 publications

(16 citation statements)

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“…However, this does not imply that there is no fluctuation in individual companies, because some laboratories were closed and new ones were founded during this period. Similar observations were reported by Kitto (1997), who described the tissue culture industry in the United States of America to be ''volatile''. For The Netherlands, an increase in the lab size and number between 1983 (28 laboratories) and 1990 (78) was reported (Pierik, 1997).…”

Section: Foundation Of Adivk (Arbeitskreis Deutsche In Vitro Kulturensupporting

confidence: 84%

Commercial in vitro plant production in Germany in 1985–2004

Winkelmann

Geier

Preil³

2006

Plant Cell Tiss Organ Cult

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Section: Foundation Of Adivk (Arbeitskreis Deutsche In Vitro Kulturensupporting

confidence: 84%

Commercial in vitro plant production in Germany in 1985–2004

Winkelmann

Geier

Preil³

2006

Plant Cell Tiss Organ Cult

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“…Nevertheless, it is a costly technology restricted to products with a very high unit value. It has been suggested that large-scale application of micropropagation largely depends on reducing production costs with automation and the use of liquid media as likely solution (Debergh, 1988;Aitken-Christie, 1991;Kitto, 1997). However, the advantages of in vitro culture in a liquid medium are often counterbalanced by technical problems such as asphyxia, hyperhydricity, shear forces and the need for complex and costly equipment (Etienne and Berthouly, 2002).…”

Section: Introductionmentioning

confidence: 99%

Cycle characteristics in a temporary immersion bioreactor affect regeneration, morphology, water and mineral status of coffee (Coffea arabica) somatic embryos

Albarrán

Bertrand

Lartaud

et al. 2005

Plant Cell Tiss Organ Cult

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Mass regeneration of Coffea arabica L. somatic embryos using a temporary immersion bioreactor was improved by optimizing the immersion cycles, i.e. both the duration and the frequency of immersions. It was demonstrated that increasing the frequency of short immersions (1 min immersions every 24, 12 and 4 h) stimulated embryo production (480, 2,094 and 3,081 embryos/1-l bioreactor, respectively) and improved quality (60, 79 and 85% of torpedo shaped embryos, respectively). On the other hand, an increase in the immersion duration (1, 5 and 15 min) inhibited embryo regeneration (from 2,094 to 428 embryos per 1-l bioreactor) and negatively affected their morphological quality (from 79 to 49% torpedo-shaped embryos) and the conversion of embryos into plants (from 70 to 33%). A 15 min immersion duration applied every 4 h produced hyperhydric symptoms in 90% of the embryos. Hyperhydric embryos were characterized by higher fresh weight and water content, more negative values for water potential and higher K + content when compared to normal torpedo-shaped embryos. Micrographs showed structural problems in the globular stage, such as the existence of an irregular epidermis and an absence of reserves. Whatever the immersion cycle used, the somatic embryos exhibited water and mineral characteristics very different from those of their zygotic counterparts. The use of 1 min immersions every 4 h led to the production of the largest quantities of torpedo-shaped embryos without hyperhydricity that succeeded in regenerating plants (75% conversion).

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“…Medicinal plants have a great importance due to their potential uses in pharmaceutical industries. Plant tissue culture is well known biotechnological tool for the rapid propagation of medicinal plants for the purpose of commercialization and conservation (1). The species which are failed in successful propagation through seeds or lacking vegetative reproduction can be multiplied through in vitro regeneration by employing this technique.…”

Section: Introductionmentioning

confidence: 99%

Direct Organogenesis of a Critically Endangered Medicinal Liana, Coscinium Fenestratum (Gaertn.) Colebr. (Menispermaceae)

Karthika¹

2019

krj

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An efficient and reproducible regeneration protocol via direct organogenesis has been established using nodal explants of Coscinium fenestratum. Nodal explants were inoculated on MS medium supplemented with BAP and TDZ at 2.0 and 1.0mg/L respectively were produced high frequency of shoots (91.01%). The elongated shoots subcultured on half strength MS medium supplemented with IBA alone at 0.6mg/L produced high frequency of roots (97.42%). Rooted plantlets were acclimatized and established in garden soil, sand and vermicompost in the ratio 1:1:1 with good survival rate under greenhouse conditions. This protocol can be efficiently used for mass multiplication and conservation of this critically endangered medicinal liana in its native habitat.

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Commercial Micropropagation

Cited by 25 publications

References 0 publications

Commercial in vitro plant production in Germany in 1985–2004

Commercial in vitro plant production in Germany in 1985–2004

Cycle characteristics in a temporary immersion bioreactor affect regeneration, morphology, water and mineral status of coffee (Coffea arabica) somatic embryos

Direct Organogenesis of a Critically Endangered Medicinal Liana, Coscinium Fenestratum (Gaertn.) Colebr. (Menispermaceae)

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