Commercial extenders and freezing curves for the preservation of sperm cells of white-tailed deer (<i>Odocoileus virginianus</i>)

Clemente-Sánchez, Fernando; Cessa-Reyes, Victoria; Cortez‐Romero, César; Tarango-Arámbula, Luis A.; Arenas-Baez, Pablo

doi:10.1080/09712119.2014.980422

Cited by 7 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This study showed that post-mortem cryopreservation of sperm cells of the Veracruz subspecies of white-tailed deer provided optimal parameters after thawing. On average, the viability (40.00%), progressive motility (35.75%), presence of cytoplasmic droplets (13.50%), and normal morphology (44.50%) are aligned with the consulted literature [7,12,13]. When evaluating the effect of age on the sperm parameters, old bucks (around 4 years) had a higher proportion of sperm cells with normal morphology (p<0.01) and cytoplasmic droplets (p<0.01) compared with younger animals (2 and 3 years old).…”

Section: Resultssupporting

confidence: 73%

Environmental Influences on Frozen Epididymal Sperm Cell Viability in White-Tailed Deer

Muñoz-Hernandez,

Lagunes-Moreno,

Barrientos-Villeda

et al. 2024

UPJOZ

View full text Add to dashboard Cite

The objective of this study was to evaluate the association between Temperature and Humidity Index (THI), age, and post-collection time with post-thawing sperm cell parameters such as progressive motility, viability, morphology, and cytoplasmic droplets. Samples were collected from 4 male white-tailed deer after regulated hunting. THI was calculated on collection days. Two THI categories were determined, ≥70 and <70. Thawed semen was placed on a slide and observed under a phase-contrast microscope to evaluate the progressively motile sperm cells. Morphology, viability, and cytoplasmic droplets were assessed on a smear stained with eosin-nigrosine and classified as normal or abnormal, viable or not viable, and present or absent, respectively. When THI was ≥70, the progressive motility (51.0 vs 30.67%), sperm viability (51.0 vs 36.33%), and the proportion of sperm cells with normal morphology (65.0 vs 37.67%) were higher than to days with a THI <70, without differences in the presence of cytoplasmic droplets. The 4-years bucks had a higher proportion of sperm cells with normal morphology (81.0 vs 35.5 and 26.0%) and cytoplasmic droplets (27.0 vs. 12.0 and 7.5%) compared to 2- and 3-year-old animals. Samples processed after 4 hours (51.0%) had a higher progressive motility than samples processed after 6 and 8 hours (32.0 and 25.0%, respectively). Spermatozoa processed after 8 hours (30.0%) had a lower viability than spermatozoa processed after 4 hours (51.0%). Compared to samples processed after 6 and 8 hours, those processed after 2 and 4 hours had a higher proportion of sperm cells with normal morphology (26.0 and 6.0 vs 81.0 and 65.0%, respectively). Samples processed after 2 hours had a higher (27.0 vs. 12.0%) proportion of sperm cells with cytoplasmic droplets than to those processed after 6 hours. High THI favors progressive motility and sperm viability. The older bucks had a greater proportion of sperm cells with normal morphology and cytoplasmic droplets than to the young ones. The greater progressive motility, viability, morphology, and presence of cytoplasmic droplets were found when the samples were processed within the first 4 hours.

show abstract

Section: Resultssupporting

confidence: 73%

Environmental Influences on Frozen Epididymal Sperm Cell Viability in White-Tailed Deer

Muñoz-Hernandez,

Lagunes-Moreno,

Barrientos-Villeda

et al. 2024

UPJOZ

View full text Add to dashboard Cite

show abstract

“…White-tailed deer is a dimorphic species; this dimorphism could be more evident in winter, since this is when the breeding season occurs and the production of testosterone increases (Clemente-Sánchez et al, 2015). Males have well-developed antlers, flared necks, and an accumulation of body fat to maintain themselves during the winter and breeding season (Dostaler, Quillet, Therrien & Côte, 2011;Galindo-Leal & Weber, 1998).…”

Section: Discussionmentioning

confidence: 99%

“…White-tailed deer (Odocoileus virginianus) is one of the most important wildlife species in Mexico due to the number of animals that are hunted annually (Clemente-Sánchez, F., Cessa-Reyes, V., Cortez-Romero, C., Tarango-Arámbula, L. A., & Arenas-Baez, 2015). Established regulations for the harvest of white-tailed deer by institutions in North America usually allow different numbers of animals to be harvested depending upon their sex, having a preference for males (Lindsay & Belant, 2008;Riley et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

White-tailed deer sex identification from faecal DNA and pellet morphometry by neural network and fuzzy logic analyses

et al. 2019

View full text Add to dashboard Cite

Knowing the sex of white-tailed deer (Odocoileus virginianus) individuals can provide information to set harvesting rates and management activities. Therefore, the aim of this study is to identify the sex through classification function by using faecal pellet morphometry. Faeces were collected for 12 months in Durango, Mexico; their morphometric variables were measured, the faecal DNA was extracted, and the SRY gene marker was amplified to identify sex. A neural network and fuzzy logic sex classification functions were obtained. The outputs were validated with the SRY gene results. Data from adults in the winter were used to obtain the classification functions. Classification functions could accurately classify sex in 94.4% with neural networks and 86.9% with fuzzy logic. The neural network classified more accurately the sex of adult white-tailed deer studied in winter with the faecal pellets morphometry than with the fuzzy logic analysis. This technique can be a tool for non-invasive studies and monitoring of populations.

show abstract

“…La movilidad puede evaluarse por métodos subjetivos y poco fiables o por métodos objetivos, mediante los sistemas CASA (Yániz et al, 2018). El análisis subjetivo de la movilidad considera la movilidad masal (Clemente-Sánchez et al, 2015), que se evalúa en semen no diluido y se valora el número, la densidad y la velocidad del movimiento de las ondas o remolinos que se forman en la superficie de la gota de semen. Cuando no se ven ondas y los espermatozoides no se mueven se asigna un valor cero (categoría inferior), se van asignando valores hasta el valor más elevado (categoría 5, según la escala), donde se observan ondas densas y con movimiento muy rápido.…”

Section: Movilidad Reducida Astenozoospermiaunclassified

Biotecnología aplicada al estudio de la movilidad del semen porcino

2021

View full text Add to dashboard Cite

Introducción. El uso de biotecnologías reproductivas ha significado una mejora relevante sobre los parámetros de eficiencia y rentabilidad en la industria porcina. Objetivo. Determinar el estado del arte en el que se encuentra la biotecnología aplicada al estudio del semen y la implementación; en granjas porcinas; de técnicas de reproducción asistida como la inseminación artificial. Desarrollo. En el sector porcino, la fertilidad de los verracos (Sus scrofa domestica) tiene un efecto significativo sobre la eficiencia general de la reproducción de las piaras. Aunque hay métodos objetivos de valoración precisa de las variables seminales de los verracos, muchas granjas aún continúan realizando sus evaluaciones de forma subjetiva. Los conceptos bioquímicos que determinan la funcionalidad del gameto masculino o espermatozoide son molecularmente complejos, sin embargo, pueden conocerse los aspectos generales de la funcionalidad y desarrollar métodos de manejo del semen que permita el mantenimiento de las condiciones óptimas de las muestras seminales o dosis de semen utilizadas en inseminación artificial (IA). Dentro de la funcionalidad espermática intervienen parámetros clásicos de volumen, concentración y movilidad que son fundamentales para la optimización de las dosis seminales producidas por verraco. Los sistemas CASA (Computerassisted semen analysis) son una aplicación biotecnológica que desplaza el análisis subjetivo tradicional del semen para la valoración de la calidad, estos aportan grandes volúmenes de información que han permitido explorar más en detalle los eyaculados porcinos. Conclusiones. En las granjas que utilizan la inseminación artificial, el uso de técnicas biotecnológicas para el análisis seminal, constituye un valor agregado para el estudio de la calidad del semen y la optimización de dosis seminales producidas.

show abstract

Commercial extenders and freezing curves for the preservation of sperm cells of white-tailed deer (Odocoileus virginianus)

Cited by 7 publications

References 13 publications

Environmental Influences on Frozen Epididymal Sperm Cell Viability in White-Tailed Deer

Environmental Influences on Frozen Epididymal Sperm Cell Viability in White-Tailed Deer

White-tailed deer sex identification from faecal DNA and pellet morphometry by neural network and fuzzy logic analyses

Biotecnología aplicada al estudio de la movilidad del semen porcino

Contact Info

Product

Resources

About