Commercial embryo transfer in the United States

McCue, P M; Troedsson, Mats H.T.

doi:10.21836/pem20030624

Cited by 2 publications

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“…Previous studies in horses (Allen 1982a, Douglas 1982McCue and Troedsson 2003;Stout 2003;Wilsher and Allen 2004) and camels (McKinnon et al 1994;Skidmore et al 2002) achieved highest pregnancy rates when the recipient ovulated 0-2 days after, not before, the donor. However, the results of the present study have demonstrated that good pregnancy rates can also be achieved using asynchronous recipient mares that have ovulated up to 3 days before the donor, provided they are treated with meclofenamic acid.…”

Section: Discussionmentioning

confidence: 84%

“…In the mare, maximum pregnancy rates are achieved when transferring embryos to recipients that ovulate +1 to -3 days with respect to the donor (Allen 1982a;Squires et al 1982;McCue and Troedsson 2003;Stout 2003;Wilsher and Allen 2004). A level of synchrony between the embryo and uterine environment is essential for the establishment of pregnancy.…”

Section: Introductionmentioning

confidence: 99%

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Meclofenamic acid extends donor-recipient asynchrony in equine embryo transfer

Wilsher¹,

Kölling²,

Allen³

2010

Equine Veterinary Journal

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Summary Reasons for performing study: A level of synchrony between embryo and uterine environment is essential for the establishment of pregnancy when performing embryo transfer. The ability to extend the acceptable degree of asynchrony would allow more efficient use of recipient mares. Objectives: To establish if administration of the prostaglandin synthetase inhibitor, meclofenamic acid, to asynchronous recipient mares could widen the acceptable window of asynchrony for embryo transfer. Hypothesis: The prostaglandin synthetase inhibitory action of meclofenamic acid may act to suppress luteolysis and thereby allow for a greater degree of asynchrony between donor and recipient mares. Methods: A total of 72 Grade 1 horse embryos were transferred nonsurgically into the uteri of recipient mares that had ovulated 2 (n = 20), 3 (n = 20), 4 (n = 16) or 5 (n = 16) days before the donor. Half of the mares in each group were treated orally with 1 g meclofenamic acid, beginning on Day 9 after ovulation and continuing for 7 days after embryo transfer. Results: Comparison of recipient:donor asynchrony between treated and untreated mares was: +2 days, 9/10 pregnancies vs. 8/10 (P = 1.00); by +3 days, 8/10 vs. 2/10 (P = 0.025); by +4 days, 5/8 vs. 1/8 (P = 0.121); and by +5 days 3/8 vs. 0/8 (P = 0.20). In 10/11 meclofenamic acid‐treated and 23/25 untreated recipient mares that failed to become pregnant, luteolysis occurred at the normal time (14–19 days) after ovulation. Conclusion: Treatment with meclofenamic acid supported the establishment of pregnancy in recipient mares that ovulated before the donors. However, meclofenamic acid appeared to provide this support in a manner other than by suppression of luteolysis. Potential relevance: Pregnancy rates can be increased in recipient mares that ovulate 3 days before the donor by administration of meclofenamic acid.

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Section: Discussionmentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Meclofenamic acid extends donor-recipient asynchrony in equine embryo transfer

Wilsher¹,

Kölling²,

Allen³

2010

Equine Veterinary Journal

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Successful transfer of day 10 horse embryos: influence of donor–recipient asynchrony on embryo development

Wilsher

Clutton-Brock²,

Allen³

2010

Reproduction

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A total of 78 day 10 horse embryos were transferred non-surgically to recipient mares that had ovulated 9, 7, 6, 5, 4, 3, 2 or 1 day after (negative asynchrony), on the same day (synchronous), or 2 or 4 days before (positive asynchrony) the donor (nZ6 or 8 mares per group). Pregnancy rates between 100% (6/6) and 63% (5/8) were seen in recipient mares that were between C2 and K6 days asynchronous. Embryo survival to the heartbeat stage declined in recipients that were K7 days asynchronous and no embryos survived in recipients that were K9 days asynchronous. Irrespective of uterine asynchrony, cessation of embryo mobility and fixation at the base of a uterine horn occurred when the conceptus was w17 days old. Conceptus growth and development was slowed when embryos were placed in negatively asynchronous uteri. At the greatest degree of negative asynchrony at which embryos survived to the heartbeat stage, i.e. K7 and K6 days, development of the embryo proper and allantois was retarded. Luteostasis was achieved in recipient mares when day 10 embryos were transferred to recipient mares at any stage of asynchrony between K9 and C2 days with respect to the donor. These results indicate that in the horse, there is a wide window for establishment of pregnancy following embryo transfer to asynchronous recipients. Although progesterone priming of the uterus to a stage equivalent to that of the transferred embryo does not appear to be a prerequisite for embryo survival, it does nonetheless influence embryonic development.Reproduction (2010) 139 575-585

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Commercial embryo transfer in the United States

Cited by 2 publications

References 0 publications

Meclofenamic acid extends donor-recipient asynchrony in equine embryo transfer

Meclofenamic acid extends donor-recipient asynchrony in equine embryo transfer

Successful transfer of day 10 horse embryos: influence of donor–recipient asynchrony on embryo development

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