Commercial cellulases from Trichoderma longibrachiatum enable a large-scale production of chito-oligosaccharides

Abstract

Chito-oligosaccharides (COSs) are a substance class of high interest due to various beneficial bioactive properties. However, detailed mechanistic and application-related investigations are limited due to the poor availability of COSs with defined structural properties. Here, we present the large-scale production of COSs with defined degree of N-acetylation using a commercial cellulase preparation from Trichoderma longibrachiatum. The enzyme preparation was found to exclusively produce COSs lacking of acetyl groups while MS/MS analysis indicated a cellobiohydrolase to be the responsible for hydrolysis with the enzyme preparation. MS and NMR analysis proved the low content of acetyl groups in the COS mix and oligomers with a degree of polymerization (DP) of 2–6 were obtained. The low cost enzyme source was further exploited for large-scale production in a 20 g batch and resulted a COSs yield of 40%. An inexpensive enzyme source for the production of bioactive COSs was successfully implemented and thorough product analysis resulted in well-defined COSs. This strategy could improve the access to this substance class for a more detailed investigation of its various biological activities.

Efficient Preparation of Chitooligosaccharide With a Potential Chitosanase Csn-SH and Its Application for Fungi Disease Protection

“…To evaluate whether Csn-SH is cleaner and more efficient than chitosanases and commercial enzymes reported previously, we compared the characteristics of chitosanases, commercial cellulase, lipase, papain, pectinase, and pepsin during the hydrolytic processes of chitosan; the examined characteristics included the dosage of enzymes, water consumption, and the value of hydrolysates and resulting profits (Table 3). The characteristics of the above enzymes were summarized according to previous publications (Lin et al, 2002;Cabrera and Van Cutsem, 2005;Roncal et al, 2007;Lee et al, 2008;Tegl et al, 2016;Zhou et al, 2020). One ton of colloidal chitosan was used as the raw material, and the corresponding concentration was obtained by using 1% acetic acid.…”

Optimization of enzymatic hydrolysis of copra meal: compositions and properties of the hydrolysate

“…Commercial enzyme preparations have been successfully used for the production of oligosaccharide mixtures to act as a prebiotic (Im et al 2016;Montilla et al 2011). Commercial cellulase preparation from Trichoderma longibrachiatum was used for large-scale production of chitooligosaccharides (Tegl et al 2016). Since the commercial enzyme preparations are relatively inexpensive, they are more practical for the large-scale production of oligosaccharides.…”

Effect of Surfactant HLB Value on Enzymatic Hydrolysis of Chitosan

“…Figure 1 shows that adding all types of surfactants significantly enhanced the enzymatic hydrolysis of chitosan. The highest increment of reducing sugar formation was In the chitosan hydrolysis reaction, a glycosidic bond cleavage produces the aldehyde group that has a reductive property [28]. The reducing sugars formation proves the presence of enzymatic hydrolysis activity.…”

“…In the chitosan hydrolysis reaction, a glycosidic bond cleavage produces the aldehyde group that has a reductive property [28]. The reducing sugars formation proves the presence of enzymatic hydrolysis activity.…”