Comments on Microelectrodes

Gesteland, Robert C.; Howland, Bradford; Lettvin, Jerome Y.; Pitts, Walter

doi:10.1109/jrproc.1959.287156

Cited by 226 publications

(80 citation statements)

References 6 publications

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“…18-25·µm; crosssectional area approx. 250-500·µm 2 ; impedance, 10-40·KΩ) placed against the sensory face of an olfactory lamella (Gesteland et al, 1959;Caprio, 1995). The electrode was r.c.-coupled (220·pF capacitor, 20·MΩ resistor) to a highimpedance probe at one input with the other input grounded via a hypodermic needle embedded in the flank musculature of the fish.…”

Section: Electrophysiological Recording Techniquesmentioning

confidence: 99%

Polyamines as olfactory stimuli in the goldfish Carassius auratus

Rolen

Sorensen

Mattson

et al. 2003

Journal of Experimental Biology

109

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SUMMARY Electrophysiological responses of goldfish olfactory receptor neurons(ORNs) and goldfish behavioral responses to polyamines were investigated in vivo. Electro-olfactogram (EOG) recordings indicated that polyamines (putrescine, cadaverine and spermine) are potent olfactory stimuli for goldfish with estimated electrophysiological thresholds of 10–8–10–7 mol l–1,similar to that for L-arginine, the most stimulatory amino acid. Although thresholds were similar, the magnitude of the EOG responses to intermediate(10–5–10–4 mol l–1)and high (10–3 mol l–1) concentrations of polyamines dwarfed the responses to amino acids and related single amine containing compounds (amylamine and butylamine). The EOG responses to 0.1 mmol l–1 putrescine, cadaverine and spermine were, respectively,4.2×, 4.3× and 10.3× the response of the standard, 0.1 mmol l–1 L-arginine. Electrophysiological cross-adaptation experiments indicated that polyamine receptor sites are independent from those to L-amino acids (alanine, arginine, glutamate, lysine, methionine and ornithine), bile salts (sodium taurocholate and taurolithocholate), the single amine containing compounds (amylamine and butylamine) and ATP. Further, the cross-adaptation experiments revealed the existence of independent receptor sites for the different polyamines tested. Pharmacological experiments suggested that polyamine odorant transduction does not primarily involve the cyclic AMP and IP3 second messenger pathways. Behavioral assays indicated that polyamines are attractants that elicit feeding behavior similar to that elicited by L-amino acids.

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Section: Electrophysiological Recording Techniquesmentioning

confidence: 99%

Polyamines as olfactory stimuli in the goldfish Carassius auratus

Rolen

Sorensen

Mattson

et al. 2003

Journal of Experimental Biology

109

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“…Single receptor responses were recorded using metal-filled electrodes described by Gesteland, Howland, Lettvin & Pitts (1959). The tips were covered with a gelatinized platinum black sponge.…”

Section: Threshold Responses Of Olfactory Receptors 425mentioning

confidence: 99%

Unitary recordings of near threshold responses of receptor cells in the olfactory mucosa of the frog.

Drongelen¹

1978

The Journal of Physiology

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“…We speculate that this is due to a rate limiting interfacial interaction or diffusion process at the liquid-solid boundary. It is striking and has also been documented in the literature 19 that the voltage noise too closely follows the J-N formula. We believe that this is due to the fact that our system, measured under conditions of negligible current flow, is close to thermal equilibrium.…”

mentioning

confidence: 63%

“…This verifies the absence of any major noise component other than J-N noise. 19 In log-log scale, S imp V is observed to be linearly dependent on f with a slope -c for all TiN electrode areas displayed. The low-frequency behavior of an EDL can be described using a phenomenological equivalent circuit component referred to as the constant phase element (CPE), 20 with Zðf Þ ¼ 1=Qðif Þ c , where i is the imaginary unit and Q is a CPE parameter.…”

mentioning

confidence: 92%