Commentary: Where to Now for Standardization of Anti-Rubella Virus IgG Testing

Dimech, Wayne

doi:10.1128/jcm.00800-16

Cited by 5 publications

(2 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some of the discordant results observed in our study might be the result of variations in the types and source of antigen used in the different assays Quantitative comparison of IgG results by Bland Altman analysis was also satisfactory (Fig 1). Some variation in rubella specific IgG results using different ELISA kits is expected and widely recognized (12). Since the 1980s, rubella virus IgG assays have been calibrated against the same WHO international standard rubella virus serum (RUBI-1-94) (12).…”

Section: Discussionmentioning

confidence: 99%

“…Some variation in rubella specific IgG results using different ELISA kits is expected and widely recognized (12). Since the 1980s, rubella virus IgG assays have been calibrated against the same WHO international standard rubella virus serum (RUBI-1-94) (12). Much of the variation, stems from the difference between the nature of the analyte and tests originally applied for determining the standard and the currently used test systems (13).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Comparison of two commercial ELISA kits for detection of rubella specific IgM in suspected congenital rubella syndrome cases and rubella IgG antibodies in a serosurvey of pregnant women

Viswanathan

George

Murhekar

et al. 2019

Diagnostic Microbiology and Infectious Disease

View full text Add to dashboard Cite

Enzyme linked immunosorbent assay (ELISA) plays an important role in laboratory confirmation of congenital rubella syndrome (CRS), postnatal rubella and seroprevalence studies in different populations. Variation of results are documented for samples tested by different commercial kits. The Enzygnost rubella ELISA, widely used in the WHO network, is expensive and not readily available. In the present study, performance of the Euroimmun ELISA was compared to the Enzygnost ELISA for detection of rubella specific IgM and IgG antibodies.Two hundred and eighty five serum samples collected from suspected CRS patients identified through a recently initiated surveillance for CRS at six sentinel hospitals and 435 serum samples from a serosurvey of pregnant women from these sites, were available for testing of rubella specific IgM and IgG antibodies respectively. Qualitative agreement (concordance percentage and Cohen's Kappa coefficient -κ) was evaluated for both IgM and IgG assays.Bland -Altman plots were used to assess the difference in quantitative agreement for IgG titers.Good qualitative agreement between the two ELISA kits was observed for detection of both anti rubella IgM (94.7% agreement and κ of 0.86) and IgG (96.3% agreement and κ of 0.84).Sensitivity and specificity of Euroimmun assays compared to Enzygnost was 100% and 93.1% for IgM and 95.9% and 100% for IgG respectively. Bland -Altman analysis for paired quantitative results of rubella specific IgG yielded a mean difference of 0.781 IU/ml with majority of values (97.1%) within ± 2 SD of the mean difference. Euroimmun ELISA provided on an average, higher titers as compared to Enzygnost.Our study findings suggest that Euroimmun ELISA may be considered for detection of rubella specific IgM in suspected CRS cases and rubella specific IgG in surveillance studies.

show abstract

Section: Discussionmentioning

confidence: 99%