Commentary

Saheed, Mustapha; Rothman, Richard E.

doi:10.1016/j.annemergmed.2015.12.018

Cited by 1 publication

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Intriguingly, we could identify further potential TraN binding sites on several Inc18 or related multi-resistance plasmids as well as on bacterial chromosomes. Inc18 plasmids have been demonstrated to be major key players in the dissemination of vanA -encoded vancomycin resistance and other determinants facilitating non-susceptibility of MRSA strains to last-line antibiotics ( 7 , 50 , 62 , 63 ). Interestingly, most investigated bacterial plasmids and bacterial chromosomes containing a potential TraN binding site not only encoded a TraN-homolog but also a putative TraA-like relaxase and putative homologs of the motor proteins TraE and TraJ.…”

Section: Discussionmentioning

confidence: 99%

“…These broad host-range plasmids are important key players in mediating transfer of resistance genes from Enterococci to several multi-drug resistant bacterial strains. The ability of Enterococcus faecalis and Enterococcus faecium strains to acquire and disseminate mobile genetic elements encoding beneficial traits, among them resistance to last-line antibiotics, further contributed to their manifestation as leading hospital pathogens ( 5 , 7 ). Broad host-range Inc18 family plasmids were observed to confer resistance to the last-line antibiotic drug vancomycin to strains of methicillin-resistant Staphylococcus aureus (MRSA) ( 8 , 9 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

TraN: A novel repressor of an Enterococcus conjugative type IV secretion system

Kohler

Goessweiner‐Mohr

Aufschnaiter

et al. 2018

Nucleic Acids Research

View full text Add to dashboard Cite

The dissemination of multi-resistant bacteria represents an enormous burden on modern healthcare. Plasmid-borne conjugative transfer is the most prevalent mechanism, requiring a type IV secretion system that enables bacteria to spread beneficial traits, such as resistance to last-line antibiotics, among different genera. Inc18 plasmids, like the Gram-positive broad host-range plasmid pIP501, are substantially involved in propagation of vancomycin resistance from Enterococci to methicillin-resistant strains of Staphylococcus aureus. Here, we identified the small cytosolic protein TraN as a repressor of the pIP501-encoded conjugative transfer system, since deletion of traN resulted in upregulation of transfer factors, leading to highly enhanced conjugative transfer. Furthermore, we report the complex structure of TraN with DNA and define the exact sequence of its binding motif. Targeting this protein–DNA interaction might represent a novel therapeutic approach against the spreading of antibiotic resistances.

show abstract