Comment on " 'Stemness': Transcriptional Profiling of Embryonic and Adult Stem Cells" and "A Stem Cell Molecular Signature" (I)

Fortunel, Nicolas O.; Otu, Hasan H.; Ng, Huck Hui; Chen, Jinhui; Mu, Xiuqian; Chevassut, Timothy; Li, Xiaoyü; Joseph, Marie; Bailey, Charles; Hatzfeld, Jacques; Hatzfeld, Antoinette; Usta, Fatih; Vega, Vinsensius B.; Long, Philip M.; Libermann, Towia A.; Lim, Bing

doi:10.1126/science.1086384

Cited by 314 publications

(251 citation statements)

References 6 publications

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“…It should be noted that Lee et al [15] used two different probe sets: a NBE-specific probe sets and a NSC-enriched probe sets. The latter was drawn from three prior different studies [19][20][21] corresponding to a set highly enriched for stem cellrelated genes. In our study, we considered the two probe sets for our comparative analysis.…”

Section: Methods Of Analysismentioning

confidence: 99%

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Identification of OLIG2 as the most specific glioblastoma stem cell marker starting from comparative analysis of data from similar DNA chip microarray platforms

et al. 2014

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Despite advances in surgical and adjuvant treatments, overall survival of glioblastoma (GBM) patients remains poor. The cancer stem cell concept suggests that a rare stem cell population, called glioma stem cells (GSCs), has high ability to self-renewal leading to recurrence in GBM. The identification of specific markers of GSCs would provide a powerful tool to detect and to characterise them in order to develop targeted therapies. We carried out a comparative analysis based on the identification of inter-study concordances to identify the genes that exhibit at best differential levels of expression between GSC-enriched cell cultures and differentiated tumour cell cultures from independent studies using DNA chip microarray technologies. We finally studied the protein expression of the marker we considered the most specific by immunohistochemistry and semi-quantitative analysis on a retrospective series of 18 GBMs. Of the selected studies, 32 genes were retained. Among them, eight genes were identified to be overexpressed in GSC-enriched cultures compared to differentiated tumour cell cultures. Finally, among the eight genes, oligodendrocyte lineage transcription factor 2 (OLIG2) was characterised by the most different expression level in the "GSC model" compared to the "differentiated tumour cells model". Our approach suggests that OLIG2 is the most specific GSC marker; additional investigations with careful considerations about methodology and strategies of validation are, however, mandatory.

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Section: Methods Of Analysismentioning

confidence: 99%

“…The latter was drawn from three prior different studies [19][20][21] and all tissue samples analysed in this study came from the archives of the Department of Pathology of the Erasme University Hospital. All patients gave informed consent before entering in the study.…”

Section: Retrospective Clinical Seriesmentioning

confidence: 99%

Identification of OLIG2 as the most specific glioblastoma stem cell marker starting from comparative analysis of data from similar DNA chip microarray platforms

et al. 2014

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“…Furthermore, integrin expression may be a key feature of the "stem cell molecular signature" (Eckfeldt et al, 2005). Integrins are heterodimers of an ␣ and a ␤ chain, and a comparison of three independent cross system stem cell transcriptional profiling studies (Ivanova et al, 2002;Ramalho-Santos et al, 2002;Fortunel et al, 2003), found that the ␣ chain of the principal integrin laminin receptor ␣6␤1 was the only shared gene expressed in all stem cells. Disruption of integrin activity in the epidermal and hematopoietic niches resulted in compromised self-renewal (Frye et al, 2003;Scott et al, 2003;Hidalgo et al, 2004;Priestley et al, 2005).…”

Section: Signaling In Adult Neural and Other Stem Cell Nichesmentioning

confidence: 99%

The microenvironment of the embryonic neural stem cell: Lessons from adult niches?

Lathia

Rao

Mattson

et al. 2007

Developmental Dynamics

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A better understanding of the signals regulating embryonic neural stem cells is clearly an important goal. However, many studies on neural stem cell biology are conducted on the slowly-dividing cells found in the adult CNS, where specialized microenvironments or niches maintain the stem cells throughout life. By contrast, the embryonic VZ is a transient structure that does not fulfill the criteria conventionally used to define niches. In this review we will examine whether, despite these differences, the signals found in other adult stem cell niches are present in the VZ. Using the similarities and differences we observe, we will reconsider whether the location of embryonic stem cell populations such as the VZ can be thought of as niches. Finally, we will ask how these lessons from the niche inform our understanding of neurodevelopmental diseases and cancers of the CNS. Developmental Dynamics 236:3267-3282, 2007.

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“…110 However, when data from the two above-mentioned publications were compared by a third party, the degree of concordance between the studies regarding the transcripts common to stem cells was low. 112,113 The published exchange of opinions on this data (the primary papers, the counter analysis, and the rebuttal) are well worth reading as a primer regarding the complex issues involved in expression profiling and the application of this technology to rare cells. [112][113][114] Other investigators have returned to similar target populations as isolation techniques, definitions of cell populations, and microarrays have improved.…”

Section: Differentiation Programs and The Transcriptomementioning

confidence: 99%

“…112,113 The published exchange of opinions on this data (the primary papers, the counter analysis, and the rebuttal) are well worth reading as a primer regarding the complex issues involved in expression profiling and the application of this technology to rare cells. [112][113][114] Other investigators have returned to similar target populations as isolation techniques, definitions of cell populations, and microarrays have improved. Using improved arrays, Fosberg et al confirmed some of the LT-HSC transcriptome elements of previous authors (e.g.,ROBO4 and Slit-like2 ligand receptor pair) and the general trend of the preservation of "stem-ness" genes in earlier cells (Hoxa5 and Hoxb5), with proliferation genes arising in more mature forms (Hoxa9).…”

Section: Differentiation Programs and The Transcriptomementioning

confidence: 99%

Gene expression changes in normal haematopoietic cells

Lionberger¹,

Stirewalt²

2009

Best Practice & Research Clinical Haematology

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The complexity of the healthy hematopoietic system is immense, and as such, one must understand the biology driving normal hematopoietic expression profiles when designing experiments and interpreting expression data that involves normal cells. This chapter seeks to present an organized approach to the use and interpretation of gene profiling in normal hematopoiesis and broadly illustrates the challenges of selecting appropriate controls for high-throughput expression studies.

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Comment on " 'Stemness': Transcriptional Profiling of Embryonic and Adult Stem Cells" and "A Stem Cell Molecular Signature" (I)

Cited by 314 publications

References 6 publications

Identification of OLIG2 as the most specific glioblastoma stem cell marker starting from comparative analysis of data from similar DNA chip microarray platforms

Identification of OLIG2 as the most specific glioblastoma stem cell marker starting from comparative analysis of data from similar DNA chip microarray platforms

The microenvironment of the embryonic neural stem cell: Lessons from adult niches?

Gene expression changes in normal haematopoietic cells

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