Comment on ‘Dengue viral infection monitoring from diagnostic to recovery using Raman spectroscopy’

Darvin, Maxim E.; Lademann, Juergen; Брандт, Н.Н.

doi:10.1088/1612-2011/13/4/048001

Cited by 3 publications

(4 citation statements)

References 43 publications

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“…Further data analysis was performed in self-programmed software based on Matlab R2016a (The MathWorks, Inc., Natick, Massachusetts). For every pre-processed spectrum, a Gaussian function was fitted to the keratin-related Amide I Raman band around 1655 cm −1 [47] by non-linear regression with non-negativity constraints, shown in figure 1. For every XY position, the depth profile of the determined 1655 cm −1 peak height as a function of depth was approached using a seventh order polynomial by linear regression, which resulted in sufficient fitting for further analysis.…”

Section: Discussionmentioning

confidence: 99%

Surface determination of 3D confocal Raman microscopy imaging of the skin

et al. 2017

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A surface determination method for the application of 3D confocal Raman microscopy on inhomogeneous skin sections has been presented, which is based on depth profiles of the keratin contribution of the acquired Raman spectra. The method was compared to two similar auto-focusing methods that are based on the intensity of the reflected excitation light and Raman spectra, respectively. The measurements were performed on hair follicles containing skin sections of porcine ears ex vivo. The surface determination on such samples is especially challenging due to their different molecular composition and surface inhomogeneity. An advantage of this method is molecular sensitivity, whereby only the surface of the sample will be detected and not the substrate of the microscope slide, in the case of disruptions during the processing of samples. A disadvantage of the method is the increased overall acquisition time if only the surface spectra are to be applied for 2D mapping.

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Section: Discussionmentioning

confidence: 99%

Surface determination of 3D confocal Raman microscopy imaging of the skin

et al. 2017

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“…Carotenoids are fat-soluble molecules with a powerful antioxidant capacity against reactive oxygen species [26][27][28]. Carotenoids are dissolved in the blood plasma [29] and have a beneficial function in the human skin [6]. Carotenoids can be quantified separately using high performance liquid chromatography [9,30] and as a total concentration using photometric absorption at 450 and 525 nm [31] or by Raman spectroscopy [29] directly in blood plasma samples.…”

Section: Determination Of Antioxidants In Blood Using Raman Spectroscopymentioning

confidence: 99%

“…Carotenoids are dissolved in the blood plasma [29] and have a beneficial function in the human skin [6]. Carotenoids can be quantified separately using high performance liquid chromatography [9,30] and as a total concentration using photometric absorption at 450 and 525 nm [31] or by Raman spectroscopy [29] directly in blood plasma samples. All these methods are widely used in practice.…”

Section: Determination Of Antioxidants In Blood Using Raman Spectroscopymentioning

confidence: 99%

Correlation between Blood Flow and Various Physiological Parameters in Human Skin

Lademann

Darvin

Meinke

et al. 2022

J-BPE

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Blood flow is an important parameter of the human organism and skin physiology. It correlates with skin temperature, penetration of active substances through the skin, delivery of antioxidants and development of disease symptoms, especially during inflammatory processes. Dr. Alexander Vasilyevich Priezzhev played a major role in the investigation of blood circulation, cell aggregation and disease correlation, as well as in the development of an aggregometer to determine the rheology of human blood flow. The following article provides an overview of the work performed at the

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“…In the validation study, a blood sample was taken and analysed for carotenoid concentrations. The transportation path of the carotenoids goes from the digestive system directly into the blood [40,41], which could be genetically dependent [42] and be influenced by the diseases [43]. In the human metabolism the carotenoid concentration in blood changes more quickly than in the skin [44].…”

Section: Measurement Of Carotenoids In the Bloodmentioning

confidence: 99%

Multiple spatially resolved reflection spectroscopy forin vivodetermination of carotenoids in human skin and blood

Darvin

Magnussen²,

Lademann

et al. 2016

Laser Phys. Lett.

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Non-invasive measurement of carotenoid antioxidants in human skin is one of the important tasks to investigate the skin physiology in vivo. Resonance Raman spectroscopy and reflection spectroscopy are the most frequently used non-invasive techniques in dermatology and skin physiology. In the present study, an improved method based on multiple spatially resolved reflection spectroscopy (MSRRS) was introduced. The results obtained were compared with those obtained using the 'gold standard' resonance Raman spectroscopy method and showed strong correlations for the total carotenoid concentration (R = 0.83) as well as for lycopene (R = 0.80). The measurement stability was confirmed to be better than 10% within the total temperature range from 5 °C to + 30 °C and pressure contact between the skin and the MSRRS sensor from 800 Pa to 18 000 Pa. In addition, blood samples taken from the subjects were analyzed for carotenoid concentrations. The MSRRS sensor was calibrated on the blood carotenoid concentrations resulting in being able to predict with a correlation of R = 0.79. On the basis of blood carotenoids it could be demonstrated that the MSRRS cutaneous measurements are not influenced by Fitzpatrick skin types I-VI. The MSRRS sensor is commercially available under the brand name biozoom.

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Comment on ‘Dengue viral infection monitoring from diagnostic to recovery using Raman spectroscopy’

Cited by 3 publications

References 43 publications

Surface determination of 3D confocal Raman microscopy imaging of the skin

Surface determination of 3D confocal Raman microscopy imaging of the skin

Correlation between Blood Flow and Various Physiological Parameters in Human Skin

Multiple spatially resolved reflection spectroscopy forin vivodetermination of carotenoids in human skin and blood

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