Comment les interneurones perdent la cadence dans le cerveau des souris modèles d’Alzheimer

Verret, Laure

doi:10.1051/medsci/20122812008

Cited by 3 publications

(1 citation statement)

References 11 publications

(23 reference statements)

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“…One of the main clinical outcomes of AD, other than memory impairment and cognitive decline, is seizure: in fact, AD patients suffer from a higher incidence of seizure (Amatniek et al, 2006 ). In relation to this clinical observation, recent work was conducted in our and other labs, focusing on the characterization of the disruption of hippocampal and cortical neuronal synchronized activity in models of AD (Palop et al, 2007 ; Minkeviciene et al, 2009 ; Putcha et al, 2011 ; Verret, 2012 ; Corbett et al, 2013 ; Born et al, 2014 ; Davis et al, 2014 ); further research efforts were also spent on the characterization of the intrinsic excitability (IE) alterations of neuronal properties in mouse models of amyloidopathy, such as PSAPP (Brown et al, 2011 ), PDAPP (Kerrigan et al, 2014 ), CRND8 (Wykes et al, 2012 ), 5xFAD (Kaczorowski et al, 2011 ), APPSwe/PS1dE9 (Kellner et al, 2014 ), soluble oligomers of Aβ 1–42 (Scala et al, 2015 ; Tamagnini et al, 2015 ), 3xTg APPSwe/PS1/P301L (Scala et al, 2015 ), Tg2576 (Brown et al, 2011 ; Nenov et al, 2015 ) and APP23xPS45 (Busche et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Altered intrinsic excitability of hippocampal CA1 pyramidal neurons in aged PDAPP mice

Tamagnini

Novelia

Kerrigan

et al. 2015

Front. Cell. Neurosci.

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Amyloidopathy involves the accumulation of insoluble amyloid β (Aβ) species in the brain’s parenchyma and is a key histopathological hallmark of Alzheimer’s disease (AD). Work on transgenic mice that overexpress Aβ suggests that elevated Aβ levels in the brain are associated with aberrant epileptiform activity and increased intrinsic excitability (IE) of CA1 hippocampal neurons. In this study we examined if similar changes could be observed in hippocampal CA1 pyramidal neurons from aged PDAPP mice (20–23 month old, Indiana mutation: V717F on APP gene) compared to their age-matched wild-type littermate controls. Whole-cell current clamp recordings revealed that sub-threshold intrinsic properties, such as input resistance, resting membrane potential and hyperpolarization activated “sag” were unaffected, but capacitance was significantly decreased in the transgenic animals. No differences between genotypes were observed in the overall number of action potentials (AP) elicited by 500 ms supra-threshold current stimuli. PDAPP neurons, however, exhibited higher instantaneous firing frequencies after accommodation in response to high intensity current injections. The AP waveform was narrower and shorter in amplitude in PDAPP mice: these changes, according to our in silico model of a CA1/3 pyramidal neuron, depended on the respective increase and reduction of K+ and Na+ voltage-gated channels maximal conductances. Finally, the after-hyperpolarization, seen after the first AP evoked by a +300 pA current injection and after 50 Hz AP bursts, was more pronounced in PDAPP mice. These data show that Aβ-overexpression in aged mice altered the capacitance, the neuronal firing and the AP waveform of CA1 pyramidal neurons. Some of these findings are consistent with previous work on younger PDAPP; they also show important differences that can be potentially ascribed to the interaction between amyloidopathy and ageing. Such a change of IE properties over time underlies that the increased incidence of seizure observed in AD patients might rely on different mechanistic pathways during progression of the disease.

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