Commensal Propionibacterium strain UF1 mitigates intestinal inflammation via Th17 cell regulation

Colliou, Natacha; Ge, Yong; Sahay, Bikash; Gong, Minghao; Zadeh, Mojgan; Owen, Jennifer L.; Neu, Josef; Farmerie, William G.; Alonzo, Francis; Liu, Ken; Jones, Dean P.; Li, Shuzhao; Mohamadzadeh, Mansour

doi:10.1172/jci95376

Cited by 63 publications

(84 citation statements)

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“…Consequently, Bifidobacterium depletion might contribute to the dysregulation of the immune system found in hypertensive l ‐NAME‐treated rats. In addition, increased Propionibacterium was also detected in gut microbiota from l ‐NAME group, which also influence intestinal immunity increasing Th17 population in MLN . However, our study did not clarify either the mechanisms involved in dysbiosis induced by NO synthesis inhibition or if changes in gut microbiota induced by l ‐NAME are involved in the raise of BP.…”

Section: Discussionmentioning

confidence: 57%

“…By preventing barrier compromise LC40 treatment may reduce activation and migration of APC to the MLN, hence suppressing IL‐6 production and allowing for Treg to produce IL‐10, and reducing Th17 to produce IL‐17a. In addition, dihydrolipoamide acetyltransferase, one of the major proteins found in bacterial surface layer of Propionibacterium , has been described to stimulate intestinal APC, leading to T cell activation and Th17 differentiation . The reduction of Propionibacterium count in the gut microbiota induced by LC40 treatment might be involved in the lesser Th17 population found in MLN from l ‐NAME–LC40 group, as a result of inhibition of this pathway.…”

Section: Discussionmentioning

confidence: 99%

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The Probiotic Lactobacillus fermentum Prevents Dysbiosis and Vascular Oxidative Stress in Rats with Hypertension Induced by Chronic Nitric Oxide Blockade

Robles-Vera

Toral

Visitación

et al. 2018

Molecular Nutrition Food Res

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Section: Discussionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 99%

The Probiotic Lactobacillus fermentum Prevents Dysbiosis and Vascular Oxidative Stress in Rats with Hypertension Induced by Chronic Nitric Oxide Blockade

Robles-Vera

Toral

Visitación

et al. 2018

Molecular Nutrition Food Res

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“…Additionally, Bifidobacterium , Faecalibacterium , Ruminococcus , and Prevotella were inversely related to low‐grade mucosal inflammation . Propionibacterium mitigated intestinal inflammation via Th17 cell regulation, which contributed to mucosal homeostasis and disorders in SIBO state. As for microbial function, there were mainly increased activity in pathways like carbohydrate and lipid metabolism, amino acid and nucleic acid biosynthesis, which restricted nutrient transport and uptake .…”

Section: Discussionmentioning

confidence: 99%

Duodenal and rectal mucosal microbiota related to small intestinal bacterial overgrowth in diarrhea‐predominant irritable bowel syndrome

Yang

Zhang

Hong

et al. 2019

J of Gastro and Hepatol

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Key wordsglucose hydrogen breath test, irritable bowel syndrome, microbial dysbiosis index, mucosalassociated microbiota, small intestinal bacterial overgrowth. AbstractBackground and Aim: Small intestinal bacterial overgrowth (SIBO) has been proposed as an etiologic factor in irritable bowel syndrome, particularly the diarrhea-predominant subtype (IBS-D). We aimed to identify potential intestinal microbial pattern in IBS-D patients with SIBO. Methods: Diarrhea-predominant irritable bowel syndrome patients fulfilling Rome III criteria were recruited and randomly divided into an exploratory cohort (57 cases) and a validation cohort (20 cases). SIBO was identified according to standard glucose hydrogen breath test. For 16S rRNA gene sequencing, samples of duodenal mucosa, duodenal fluid, rectal mucosa, and fresh feces were collected and performed. The α and β diversity, as well as differences in microbial composition and function, in SIBO + and SIBO À IBS-D subjects were evaluated. Results: The microbial diversity and composition obviously differed between SIBO + and SIBO À IBS-D in duodenal and rectal mucosa but not in duodenal fluid and fresh feces. For rectal mucosal microbiota, it displayed markedly reduced aerobe and Gram-negative bacteria and increased facultative anaerobe and Gram-positive bacteria, moreover, altered functions of microbial metabolism in SIBO + IBS-D. Significantly higher rectal mucosarelated microbial dysbiosis index was observed in SIBO + IBS-D, and a cut-off value at À0.37 had a sensitivity of 56.55% and specificity of 90.91% to identify the SIBO in IBS-D subjects. Conclusions: Mucosal microbiota, rather than luminal bacteria, has a more apparent dysbiosis in SIBO + IBS-D patients relative to those without SIBO. Rectal mucosaassociated microbiota may act as a potential predictor of SIBO in IBS-D patients.

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“…As JS22 was shown to lack the evolutionary counterpart for SlpB (Deptula et al, 2017b), and this strain was not able to bind hydrophobic material, we conclude that the expression of SlpB at the cell surface of JS14 is the key factor mediating the adherent growth (biofilm formation) and binding of this strain to hydrophobic material. Of note, a moonlighting enzyme, dihydrolipoamide acetyltransferase was recently shown to be associated with the S-layer proteins in a commensal P. freudenreichii strain (UF1), from where it conferred increased protection against pathogen infection (Colliou et al, 2017). In the present study, a moonlighting PepN was another protein released together with SlpB from JS14; whether this aminopeptidase was associated with SlpB or whether this protein confers adaptation, for example to environments containing milk casein, or adherence to hydrophilic/-phobic surfaces remains to be shown.…”

Section: Discussionmentioning

confidence: 99%

Secretome profiling of Propionibacterium freudenreichii reveals highly variable responses even among the closely related strains

Frohnmeyer

Deptula

Nyman

et al. 2018

Microbial Biotechnology

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SummaryThis study compared the secretomes (proteins exported out of the cell) of Propionibacterium freudenreichii of different origin to identify plausible adaptation factors. Phylosecretomics indicated strain‐specific variation in secretion of adhesins/invasins (SlpA, InlA), cell‐wall hydrolysing (NlpC60 peptidase, transglycosylase), protective (RpfB) and moonlighting (DnaK, GroEL, GaPDH, IDH, ENO, ClpB) enzymes and/or proteins. Detailed secretome comparison suggested that one of the cereal strains (JS14) released a tip fimbrillin (FimB) in to the extracellular milieu, which was in line with the electron microscopy and genomic analyses, indicating the lack of surface‐associated fimbrial‐like structures, predicting a mutated type‐2 fimbrial gene cluster (fimB‐fimA‐srtC2) and production of anchorless FimB. Instead, the cereal strain produced high amounts of SlpB that tentatively mediated adherent growth on hydrophilic surface and adherence to hydrophobic material. One of the dairy strains (JS22), producing non‐covalently bound surface‐proteins (LspA, ClpB, AraI) and releasing SlpA and InlA into the culture medium, was found to form clumps under physiological conditions. The JS22 strain lacked SlpB and displayed a non‐clumping and biofilm‐forming phenotype only under conditions of increased ionic strength (300 mM NaCl). However, this strain cultured under the same conditions was not adherent to hydrophobic support, which supports the contributory role of SlpB in mediating hydrophobic interactions. Thus, this study reports significant secretome variation in P. freudenreichii and suggests that strain‐specific differences in protein export, modification and protein–protein interactions have been the driving forces behind the adaptation of this bacterial species.

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Commensal Propionibacterium strain UF1 mitigates intestinal inflammation via Th17 cell regulation

Cited by 63 publications

References 100 publications

The Probiotic Lactobacillus fermentum Prevents Dysbiosis and Vascular Oxidative Stress in Rats with Hypertension Induced by Chronic Nitric Oxide Blockade

The Probiotic Lactobacillus fermentum Prevents Dysbiosis and Vascular Oxidative Stress in Rats with Hypertension Induced by Chronic Nitric Oxide Blockade

Duodenal and rectal mucosal microbiota related to small intestinal bacterial overgrowth in diarrhea‐predominant irritable bowel syndrome

Secretome profiling of Propionibacterium freudenreichii reveals highly variable responses even among the closely related strains

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