Commensal bacteria and epithelial cross talk in the developing intestine

Rautava, Samuli; Walker, W. Allan

doi:10.1007/s11894-007-0047-7

Cited by 44 publications

(34 citation statements)

References 55 publications

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“…Normal gut flora has mainly been studied as a positive force in tissue homeostasis and immune maturation (2,3,49). Bacterial colonization stimulates the formation of Peyer's patches and mucosal lymphocyte maturation in germ-free mice (50), and symbiotic flora regulates postnatal gut development and host physiology, including the degradation of otherwise indigestible plant polysaccharides and dietary oxalates, the biotransformation of conjugated bile acids, or vitamin synthesis (51).…”

Section: Methodsmentioning

confidence: 99%

Bacterial control of host gene expression through RNA polymerase II

Lutay¹,

Ambite²,

Hernández³

et al. 2013

J. Clin. Invest.

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The normal flora furnishes the host with ecological barriers that prevent pathogen attack while maintaining tissue homeostasis. Urinary tract infections (UTIs) constitute a highly relevant model of microbial adaptation in which some patients infected with Escherichia coli develop acute pyelonephritis, while other patients with bacteriuria exhibit an asymptomatic carrier state similar to bacterial commensalism. It remains unclear if the lack of destructive inflammation merely reflects low virulence or if carrier strains actively inhibit disease-associated responses in the host. Here, we identify a new mechanism of bacterial adaptation through broad suppression of RNA polymerase II-dependent (Pol II-dependent) host gene expression. Over 60% of all genes were suppressed 24 hours after human inoculation with the prototype asymptomatic bacteriuria (ABU) strain E. coli 83972, and inhibition was verified by infection of human cells. Specific repressors and activators of Pol II-dependent transcription were modified, Pol II phosphorylation was inhibited, and pathogen-specific signaling was suppressed in cell lines and inoculated patients. An increased frequency of strains inhibiting Pol II was epidemiologically verified in ABU and fecal strains compared with acute pyelonephritis, and a Pol II antagonist suppressed the disease-associated host response. These results suggest that by manipulating host gene expression, ABU strains promote tissue integrity while inhibiting pathology. Such bacterial modulation of host gene expression may be essential to sustain asymptomatic bacterial carriage by ensuring that potentially destructive immune activation will not occur.

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Section: Methodsmentioning

confidence: 99%

Bacterial control of host gene expression through RNA polymerase II

Lutay¹,

Ambite²,

Hernández³

et al. 2013

J. Clin. Invest.

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“…Disrupted interaction of the host immune system with the commensal microflora, a major component of the intestinal microenvironment that contributes to mucosal homeostasis, 25 probably contributes to the development of human IBD. 26 To determine whether inflammation and tumor development in Stat3-IKO mice were dependent on the intestinal microflora, Stat3-IKO and Stat3 f/f control mice were treated with a combination of antibiotics (VNMA) to substantially reduce the bacterial content of the intestinal lumen.…”

Section: Inflammation and Tumor Development In Stat3-iko Colon Are Dementioning

confidence: 99%

A Novel Mouse Model of Inflammatory Bowel Disease Links Mammalian Target of Rapamycin-Dependent Hyperproliferation of Colonic Epithelium to Inflammation-Associated Tumorigenesis

Deng

Zhou

Sellers

et al. 2010

The American Journal of Pathology

198

161

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“…However, although an intrinsic rather than a food-driven stimulus may be suggested to control the changes of expression of proteins involved in the mentioned metabolic processes, other external stimuli should also be considered. These include the composition of the microbiota as well as the development of the immune system, which through cross talk with the IECs may provide maturational signals (30). Supporting a role of microbiota, fatty acid metabolism in piglets has been shown to be influenced by the presence of specific bacteria (1).…”

Section: Establishment Of Label-free Quantitative Proteomics Of In-mentioning

confidence: 99%

Time-resolved Quantitative Proteome Analysis of In Vivo Intestinal Development

Hansson

Panchaud

Favre

et al. 2011

Molecular & Cellular Proteomics

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Postnatal intestinal development is a very dynamic process characterized by substantial morphological changes that coincide with functional adaption to the nutritional change from a diet rich in fat (milk) to a diet rich in carbohydrates on from weaning. Time-resolved studies of intestinal development have so far been limited to investigation at the transcription level or to single or few proteins at a time. In the present study, we elucidate proteomic changes of primary intestinal epithelial cells from jejunum during early suckling (1-7 days of age), middle suckling (7-14 days), and weaning period (14 -35 days) in mice, using a label-free proteomics approach. We show differential expression of 520 proteins during intestinal development and a pronounced change of the proteome during the middle suckling period and weaning. Proteins involved in several metabolic processes were found differentially expressed along the development. The temporal expression profiles of enzymes of the glycolysis were found to correlate with the increase in carbohydrate uptake at weaning, whereas the abundance changes of proteins involved in fatty acid metabolism as well as lactose metabolism indicated a nondiet driven preparation for the nutritional change at weaning. Further, we report the developmental abundance changes of proteins playing a vital role in the neonatal acquisition of passive immunity. In addition, different isoforms of several proteins were quantified, which may contribute to a better understanding of the roles of the specific isoforms in the small intestine. In summary, we provide a first, time-resolved proteome

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Commensal bacteria and epithelial cross talk in the developing intestine

Cited by 44 publications

References 55 publications

Bacterial control of host gene expression through RNA polymerase II

Bacterial control of host gene expression through RNA polymerase II

A Novel Mouse Model of Inflammatory Bowel Disease Links Mammalian Target of Rapamycin-Dependent Hyperproliferation of Colonic Epithelium to Inflammation-Associated Tumorigenesis

Time-resolved Quantitative Proteome Analysis of In Vivo Intestinal Development

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