Commensal bacteria act as a broad genetic buffer in Drosophila during chronic under-nutrition

Abstract

Summary 30Eukaryotic genomes encode several well-studied buffering mechanisms that robustly 31 maintain invariant phenotypic outcome despite fluctuating environmental conditions. . CC-BY-NC 4.0 International license It is made available under a (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint . http://dx.doi.org/10.1101/334342 doi: bioRxiv preprint first posted online May. 30, 2018; (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. Results and DiscussionsThe copyright holder for this preprint . http://dx.doi.org/10.1101/334342 doi: bioRxiv preprint first posted online May. 30, 2018; genetically diverse host population facing a nutritional challenge, hence qualifying the 166 gut microbiota as a previously unappreciated buffering agent of cryptic genetic variation. GF population should in principle also exhibit greater phenotypic variation. We therefore 186 examined the variances in pupariation timing and adult emergence in the F 2 progeny of 187 the inter-DGRP strain crosses (Fig.S3a). First, individual GF larvae pupariated and 188 eclosed later, but the variances in the pooled data were greater than that of mono-189 associated counterparts ( Fig.2c and 2d); from each vial containing an equal number of 190 larvae, the variances of pupariation and eclosion were also greater in the GF samples 191( Fig.2e and 2f). Therefore, both inter-individual and among-population variances in 192 developmental timing and adult emergence are reduced. Lastly, GF adults were slightly 193 shorter (Fig.2g); the sizes of representative organs, expressed as area of the eye and the 194 wing, were also smaller, yet the variances in these traits were greater (Fig.2h, Fig.S3g). 195Furthermore, the wing/body-length allometric slopes remained unaltered, but the 196individual GF values were more dispersed along the slope (Fig.S3i,j); when taken as a 197 ratio (wing length/body-length), the variance was greater in the GF flies (Fig.S3h) 30, 2018; buffer that confers phenotypic homogeneity in various physical fitness traits in a 200 genetically diverse host population. (Fig.3a). The incidence of wing4.0 International license It is made available under a (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint . http://dx.doi.org/10.1101/334342 doi: bioRxiv preprint first posted online May. 211anomalies differed according to the genotype, and females were more affected than males 212( Fig.3b). In contrast, the most visible "defect" in their Lp WJL associated siblings, if any, 213were rare and hardly discernable (Fig.3a, Fig.S4a) (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint . http://dx.doi.org/10.1101/334342 doi: bioRxiv preprin...

Dietary Adaptation of Microbiota in Drosophila Requires NF-κB-Dependent Control of the Translational Regulator 4E-BP

“…Although diet is one of the major driving forces behind symbiotic host-bacterial associations, less is known about host signaling mechanisms that may influence diet-microbiota interactions. Genetic and genomic analyses across taxa have provided clear evidence for the importance of host genetic control in shaping microbiota diversity (Goodman et al, 2009;Ma et al, 2019). Specifically, host innate and adaptive immune signaling systems appear to play a central role in the persistence of symbiotic intestinal microbiota (Broderick and Lemaitre, 2012;Charroux and Royet, 2012;Douglas, 2011;Goodman et al, 2009;Vijay-Kumar et al, 2010).…”