Cometabolic degradation of 2- and 3-chloroaniline because of glucose metabolism byRhodococcus sp. An 117

Schukat, B.; Janke, Dieter; Krebs, D.; Fritsche, W.

doi:10.1007/bf01568913

Cited by 38 publications

(22 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…During the dioxygenation of 3-CA, theoretically two different intermediates may be formed, e.g., 3-chlorocatechol and 4-chlorocatechol (32). The MS results obtained in this study, together with data from the literature (34,50,65), suggest that D. acidovorans B8c degrades 3-CA preferably through 4-chlorocatechol. D. acidovorans LME1 showed no accumulation of chlorinated catechols, probably because of the high level of activity of a chlorocatechol dioxygenase (34).…”

Section: Discussionsupporting

confidence: 52%

“…However, it is not clear if the genes and enzymes responsible for the transformation of aniline and 3-CA into chlorocatechol (oxidative deamination) are also different. Some aniline-degrading bacteria were able to transform 3-CA into chlorocatechol, but these bacteria needed aniline or glucose as a cosubstrate and the cells had to be preincubated with aniline (45,50). On the one hand, evidence in support of the hypothesis that the oxidative deamination of aniline and its chlorinated analogue is performed by the same enzyme was provided by the work of Latorre et al (32).…”

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Genetic Diversity among 3-Chloroaniline- and Aniline-Degrading Strains of the Comamonadaceae

Boon

Goris

Vos

et al. 2001

Appl Environ Microbiol

154

141

View full text Add to dashboard Cite

We examined the diversity of the plasmids and of the gene tdnQ, involved in the oxidative deamination of aniline, in five bacterial strains that are able to metabolize both aniline and 3-chloroaniline (3-CA). Three strains have been described and identified previously, i.e., Comamonas testosteroni I2 and Delftia acidovorans CA28 and BN3.1. Strains LME1 and B8c were isolated in this study from linuron-treated soil and from a wastewater treatment plant, respectively, and were both identified as D. acidovorans. Both Delftia and Comamonas belong to the family Comamonadaceae. All five strains possess a large plasmid of ca. 100 kb, but the plasmids from only four strains could be transferred to a recipient strain by selection on aniline or 3-CA as a sole source of carbon and/or nitrogen. Plasmid transfer experiments and Southern hybridization revealed that the plasmid of strain I2 was responsible for total aniline but not 3-CA degradation, while the plasmids of strains LME1 and B8c were responsible only for the oxidative deamination of aniline. Several transconjugant clones that had received the plasmid from strain CA28 showed different degradative capacities: all transconjugants could use aniline as a nitrogen source, while only some of the transconjugants could deaminate 3-CA. For all four plasmids, the IS1071 insertion sequence of Tn5271 was found to be located on a 1.4-kb restriction fragment, which also hybridized with the tdnQ probe. This result suggests the involvement of this insertion sequence element in the dissemination of aniline degradation genes in the environment. By use of specific primers for the tdnQ gene from Pseudomonas putida UCC22, the diversity of the PCR-amplified fragments in the five strains was examined by denaturing gradient gel electrophoresis (DGGE). With DGGE, three different clusters of the tdnQ fragment could be distinguished. Sequencing data showed that the tdnQ sequences of I2, LME1, B8c, and CA28 were very closely related, while the tdnQ sequences of BN3.1 and P. putida UCC22 were only about 83% identical to the other sequences. Northern hybridization revealed that the tdnQ gene is transcribed only in the presence of aniline and not when only 3-CA is present.For many years, anilines and chloroanilines have been among the most important industrially produced amines. They are used widely in the production of polyurethanes, rubber, azo dyes, drugs, photographic chemicals, varnishes, and pesticides (20,29). As a consequence of this widespread use, they are detected in wastewaters (31, 49). Moreover, chloroanilines have been found in waters as a consequence of the transformation of frequently used acetamide and urea herbicides (31). These toxic and recalcitrant compounds are considered important environmental pollutants (37) and are subject to legislative control by the 76/464/EEC Directive (13) and by the Priority Pollutant List of the U.S. Environmental Protection Agency (15).In aquatic environments, the major way to remove aniline is through biodegradation (20,35). The first step of t...

show abstract

Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 98%

Genetic Diversity among 3-Chloroaniline- and Aniline-Degrading Strains of the Comamonadaceae

Boon

Goris

Vos

et al. 2001

Appl Environ Microbiol

154

141

View full text Add to dashboard Cite

show abstract

“…The induction of aniline oxidizing activity in Rhodococcus erythropolis AN-13 was accelerated in the presence of glucose with an increase in cell growth (Aoki et al, 1983). Shukat et al (1983) reported that Rhodococcus sp. AN117 was able to co metabolize 2-and 3-chloroaniline in the presence of glucose and that the addition of supplemental glucose did not have any inhibitory effect on the rate of aniline degradation.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of a bacterial strain for aniline degradation

Ahmed¹,

Ahmed²,

Nisar³

et al. 2010

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Aniline, a serious environmental threat and health risk to living organisms is being released into the soil and water bodies owing to its expanded use in industry. The objective of the present study was to isolate a strain from rhizospheric soil samples of wheat (Triticum aestivum L.) taken from an agricultural site near the industrial area of Faisalabad, with the capability of degrading aniline with its maximum activity. The isolated strain was identified as Staphylococcus aureus ST1 a newly reported strain for aniline degradation. The strain ST1 showed tolerance up to 2000 ppm for aniline on mineral salt media plates and its degradative ability was checked through shake flasks experiments using HPLC. The strain was capable of degrading aniline and utilizing it as a sole source of carbon and energy. Maximum reduction of aniline concentration in medium up to 59.65% was observed after 72 h. An enhancement in biodegradation was observed using glucose as an additional growth substrate. The degradative products analyzed by HPLC were catechol, phenol and some other unknown compounds. Plasmid curing showed the involvement of plasmid encoded genes which was later followed by the isolation of plasmid DNA, which was found to be a large one of ~40 kb having restriction sites for enzymes (EcoRI, BamHI, ClaI, StuI, PstI, and HindIII) used.

show abstract

“…Some researchers had studied on the biodegradation of aniline and a few species of anilinebiodegrading bacteria including Pseudomonas [11], Moraxella [12], Comamonas [13], Rhodococcus [14], Frateuria [15], Nocardia [16] and Achromobacter [17] were successfully isolated from environment. As for the biodegradation pathway of aniline, it showed that aniline could firstly be converted via aniline dioxygenase to catechol in the aerobic environment, and then catechol was further biodegraded to cis,cis-muconic acid (ccMA) catalyzed by the catechol 1,2-dioxygenase (the ortho-cleavage pathway) or 2-hydroxymuconic semialdehyde by catechol 2,3-dioxygenase (the metacleavage pathway) [8,11,18,19].…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, aniline could be metabolized via p-aminobenzoate as the first intermediate under anaerobic denitrifying conditions [20]. Furthermore, the genes for the biodegradation of aniline was cloned and identified [14,21].…”

Section: Introductionmentioning

confidence: 99%