Cometabolic biotransformation of fenpropathrin by Clostridium species strain ZP3

Abstract

A novel bacterial strain capable of degrading the pyrethroid pesticide fenpropathrin was isolated from mixed wastewater and sludge samples. Phylogenetic analysis of the 16S rDNA sequence revealed that the organism belongs to the genus Clostridium. The organism can co-metabolically transform fenpropathrin at 100 mg l(-1) at 35°C and pH 7.5 in 12 days. Metabolic products of fenpropathrin from strain ZP3 were examined by gas chromatography/mass spectrometry, and the results showed that the organism degraded fenpropathrin with an oxidization process to yield benzyl alcohol, benzenemethanol, 3,5-dimethylamphetamine. Analyses of cell-free extracts from this strain showed that the optimal degrading conditions for degrading fenpropathrin were 35°C and pH 7.5, and degradation efficiency was 20.0 mg l(-1) day(-1), and it might be potential using for rapid treating fenpropathrin, for example, on the surface of fruits and vegetables.

Spirodela polyrhiza stimulates the growth of its endophytes but differentially increases their fenpropathrin-degradation capabilities

“…This result was different from that of Clostridium sp. ZP3, which exhibits high efficiency in degrading fenpropathrin in the presence of glucose (Zhang et al, 2011). Although the plant exudates and extracts can offer several bioavailable substrates, their relatively smaller concentrations lead to a less significant increase in bacterial growth and degradation of fenpropathrin, as expected.…”

“…The bacterial growth was monitored by measuring the absorbance at OD 600 by a spectrophotometer (UV-1700; Shimadzu). Fenpropathrin residuals in the water phase were extracted and quantified as per the method of Zhang et al (2011) with a slight modification. Briefly, the water phase was extracted twice with an equal volume of dichloromethane.…”

“…, Brevibacterium aureum (Chen et al, 2013), and Clostridium sp. ZP3 (Zhang et al, 2011). Fan et al (2012) have isolated and identified a pyrethroid-degrading enzyme, Sys410, from soil in the Turban Basin by constructing a metagenomic library.…”

Kinetics and Mechanism of Fenpropathrin Biodegradation by a Newly Isolated Pseudomonas aeruginosa sp. Strain JQ-41

“…Noticeably, the isolate experienced a prolonged slow growth phase for utilizing fenpropathrin at higher initial concentrations (over 100 mg l -1 ), but was not completely inhibited even at concentration as high as 800 mg l -1 . However, studies in the past reported that the fenpropathrin-degrading strains usually transform the pesticides with concentration lower than 200 mg l -1 [4,34]. This feature gives the isolate a competitive advantage in diverse environments, especially exposed to a high concentration of fenpropathrin pollution.…”

“…To date, a few fenpropathrin-degrading microorganisms such as Sphingobium cloacae [4], Clostridium celerecrescens [34], Ochrobactrum tritici [31], and Haihai Song and Zhiren Zhou have equally contributed to this work.…”