Cometabolic biotransformation and impacts of the anti-inflammatory drug diclofenac on activated sludge microbial communities

Nguyen, Dinh Duc; Pramanik, Biplob Kumar; Oh, Sung-Tag

doi:10.1016/j.scitotenv.2018.12.094

Cited by 48 publications

(25 citation statements)

References 36 publications

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“…According to Nguyen et al [7], biodegradation and adsorption were the main removal pathways of DCF in activated sludge, and biodegradation (less than 50% in the study) occurred via cometabolic degradation rather than direct metabolism. An efficient biodegradation path for DCF could lead to the development of low-cost biological removal processes.…”

Section: Introductionmentioning

confidence: 71%

“…Biotransformation of 70% of DCF supplied as the sole carbon source was also biodegraded by Labrys portucalensis F11 after 30 days [30]. Finally, a study of Nguyen et al [7] revealed the functional capacity of Nitratireductor, Asticcacaulis, and Pseudoxanthomonas in the cometabolism of DCF but required further experimental investigations to confirm it. Compared with the contribution of adsorption on the surface of the fungal cells observed in previous studies, the results obtained with the strains DCF1, DCF2, and DIU2 revealed that adsorption, calculated as the difference in DCF concentration between the azide-killed controls and the uninoculated ones, was lower than 13%.…”

Section: Discussionmentioning

confidence: 96%

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Biological Removal and Fate Assessment of Diclofenac Using Bacillus subtilis and Brevibacillus laterosporus Strains and Ecotoxicological Effects of Diclofenac and 4′-Hydroxy-diclofenac

Grandclement

Piram

Petit

et al. 2020

Journal of Chemistry

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Since bacterial consortia involved in conventional wastewater treatment processes are not efficient in removing diclofenac (DCF), an emerging pollutant frequently detected in water bodies, the identification of microorganisms able to metabolise this pharmaceutical compound is relevant. Thus, DCF removal was investigated using bacteria isolated from aqueous stock solutions of this micropollutant and identified as Bacillus and Brevibacillus species using 16S rRNA gene sequencing. A 100% DCF removal was achieved after 17 hours of experiment at 20°C in a nutrient medium; the biodegradation kinetic followed a pseudo-first order (kbiol = 11 L·gSS−1·d−1). Quantitative assessment of DCF removal showed that its main route was biotic degradation. The main degradation product of DCF, 4′-hydroxy-diclofenac (4′-OH-DCF), was identified using liquid chromatography-electrospray ionisation high-resolution mass spectrometry. Since the ecotoxicological impact of 4′-hydroxy-diclofenac was not reported in the literature, the ecotoxicity of DCF and its metabolite were tentatively evaluated using Vibrio fischeri bioassays. Results from these tests showed that this metabolite is not more toxic than its parent compound and may hopefully be an intermediate product in the DCF transformation. Indeed, no significant difference in ecotoxicity was observed after 30 min between DCF (50 should be writtten in subscript all along the manuscript in EC50 = 23 ± 4 mg·L−1) and 4′-hydroxy-diclofenac (EC50 = 19 ± 2 mg·L−1). Besides, the study highlighted a limit of the Microtox® bioassay, which is largely used to assess ecotoxicity. The bioluminescence of Vibrio fischeri was impacted due to the production of microbial activity and the occurrence of some carbon source in the studied medium.

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Section: Introductionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 96%

Biological Removal and Fate Assessment of Diclofenac Using Bacillus subtilis and Brevibacillus laterosporus Strains and Ecotoxicological Effects of Diclofenac and 4′-Hydroxy-diclofenac

Grandclement

Piram

Petit

et al. 2020

Journal of Chemistry

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“…Arriaga et al reported a decrease of COD removal in conventional AS reactors fed with pharmaceuticals [19]. Considerable research has been performed on the composition of functionality of microbial communities in AS upon exposure to anthropogenic chemicals [15,16,[18][19][20][21]. Alvarino et al studied the inhibitory effects of acetaminophen and doxycycline on the activity of nitrifying, denitrifying, and anammox biomass and found significant inactivation of ammonium oxidizing and denitrifying bacteria [13].…”

Section: Introductionmentioning

confidence: 99%

Enzyme response of activated sludge to a mixture of emerging contaminants in continuous exposure

et al. 2020

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The relevant information about the impacts caused by presence of emerging pollutants in mixtures on the ecological environment, especially on the more vulnerable compartments such as activated sludge (AS) is relatively limited. This study investigated the effect of ibuprofen (IBU) and triclosan (TCS), alone and in combination to the performance and enzymatic activity of AS bacterial community. The assays were carried out in a pilot AS reactor operating for two-weeks under continuous dosage of pollutants. The microbial activity was tracked by measuring oxygen uptake rate, esterase activity, oxidative stress and antioxidant enzyme activities. It was found that IBU and TCS had no acute toxic effects on reactor biomass concentration. TCS led to significant decrease of COD removal efficiency, which dropped from 90% to 35%. Continuous exposure to IBU, TCS and their mixtures increased the activities of glutathione s-transferase (GST) and esterase as a response to oxidative damage. A high increase in GST activity was associated with non-reversible toxic damage while peaks of esterase activity combined with moderate GST increase were attributed to an adaptive response.

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“…DCF was almost completely disappeared in aerobic slurry regardless of the origins of the soils after 14 days of incubation. The high degradability of DCF in this study was quite different behavior from that in aerobic wastewater treatment where DCF is persistent in general [16,[27][28][29][30]. IBP and FEP were also degraded completely regardless of the origin of soils under aerobic condition, although several metabolites of IBP have been well known in biological degradation [31].…”

Section: Effect Of Soil Type On the Degradationmentioning

confidence: 57%

Aerobic and Anaerobic Biological Degradation of Pharmaceutically Active Compounds in Rice Paddy Soils

2019

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One of the concerns against the use of sewage sludge for agricultural purposes is emerging contaminants contained in sewage sludge. Most of the studies on biological degradation of pharmaceutically active compounds in agricultural land were carried out with water-unsaturated soils under relatively aerobic conditions. In this study, the degradation of pharmaceuticals mainly including non-steroidal anti-inflammatory drugs (NSAIDs) was investigated in Asian rice paddy soils that are flooded in anaerobic condition. The experimental results showed that the concentrations of the target pharmaceuticals excluding the exception of naproxen were poorly decreased in anaerobic condition. On the other hand, the microbial communities of the soils contained the aerobic degraders of clofibric acid and diclofenac, which are generally persistent in biological wastewater treatment. The higher degradation rates in aerobic condition suggest the possibility of enhanced degradation of pharmaceuticals by supplying oxygen with plowing anaerobic rice fields or with drying the field in off-season for farming.

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Cometabolic biotransformation and impacts of the anti-inflammatory drug diclofenac on activated sludge microbial communities

Cited by 48 publications

References 36 publications

Biological Removal and Fate Assessment of Diclofenac Using Bacillus subtilis and Brevibacillus laterosporus Strains and Ecotoxicological Effects of Diclofenac and 4′-Hydroxy-diclofenac

Biological Removal and Fate Assessment of Diclofenac Using Bacillus subtilis and Brevibacillus laterosporus Strains and Ecotoxicological Effects of Diclofenac and 4′-Hydroxy-diclofenac

Enzyme response of activated sludge to a mixture of emerging contaminants in continuous exposure

Aerobic and Anaerobic Biological Degradation of Pharmaceutically Active Compounds in Rice Paddy Soils

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