Comet assay in gill cells of Prochilodus lineatus exposed in vivo to cypermethrin

Poletta, Gisela Laura; Gigena, F.; Loteste, Alicia Elena; Parma, Maria Julieta E.; Kleinsorge, E. C.; Simoniello, María Fernanda

doi:10.1016/j.pestbp.2013.10.007

Cited by 25 publications

(10 citation statements)

References 37 publications

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“…And such ALS increases in DNA are, at least partly, resulted from the monocrotophos-induced oxidative DNA damage, as indicated by the altered activities of antioxidant enzymes which disturbed the scavenging of ROS. It is worth to note that abnormally increased DNA damage is always potent inducers of cell death and many tumors (Vamvakas et al 1997;Khanna and Jackson 2001;Jackson 2002), which can further influence the growth, development, reproduction, and population dynamics of organisms (Poletta et al 2013;Santos et al 2013;Einaudi et al 2014;Ribas-Maynou et al 2014). Considering that liposoluble monocrotophos, remaining in food and the aquatic environment, may accumulate in tissues of organisms (Arifin et al 1997), appropriate discharge standards should be established in practical use to reduce the ecological risk of this pesticide.…”

Section: Discussionmentioning

confidence: 99%

Induction of DNA base damage and strand breaks in peripheral erythrocytes and the underlying mechanism in goldfish (Carassius auratus) exposed to monocrotophos

Zhao

Wei

Zhang

et al. 2015

Fish Physiol Biochem

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Using goldfish (Carassius auratus) as the model animal, the present study revealed the types of the DNA damage induced by monocrotophos, a highly toxic organophosphorus pesticide, and explored the mechanism underlying the DNA-damaging effect of this pesticide. Results of the alkaline comet assay showed that global DNA damage (including single- and double-strand breaks and alkali-labile sites) in peripheral erythrocytes of goldfish, measured as olive tail moment, was significantly increased by exposure to 0.01, 0.10, and 1.00 mg/L monocrotophos for 24, 48, 96, and 168 h. In particular, alkali-labile sites rather than single- or double-strand breaks, distinguished by the alkaline, pH 12.1, and neutral comet assays, were mainly induced by monocrotophos at 48 h. Oxidative damage in DNA bases and telomeric DNA was investigated by using the alkaline comet assay combined with endonuclease III or formamidopyrimidine DNA glycosylase and with fluorescence in situ hybridization, respectively. Further, glutathione peroxidase activity significantly decreased at 24 h but increased at 96 and 168 h, and malondialdehyde concentrations significantly increased at 48 h but gradually decreased at 96 and 168 h, which indicated an over-production of reactive oxygen species (ROS) at short exposure durations, but effective scavenging at long exposure durations in the peripheral blood tissues. Accordingly, our results suggest that DNA damage induced by monocrotophos in fish blood cells is possibly due to the inhibition of ROS scavenging and resulted accumulation of ROS.

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Section: Discussionmentioning

confidence: 99%

Induction of DNA base damage and strand breaks in peripheral erythrocytes and the underlying mechanism in goldfish (Carassius auratus) exposed to monocrotophos

Zhao

Wei

Zhang

et al. 2015

Fish Physiol Biochem

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“…Since commercial formulations are those that really have an environmental impact, it was preferred to use this kind of presentation for this study, as in most recent reports. Additionally, it is important to remark that the recovery time of CYP exposure in the aqueous solution was similar to that prepared using the pure active ingredient (Xu and Huang, 2017) and also to that prepared using a commercial formulation (Poletta et al, 2013). In Argentina, in some cases, CYP concentrations exceed aquatic ecological guidelines (Carriquiriborde et al, 2007;Jergentz et al, 2005;Mugni et al, 2010), so non-target fauna could be affected at different biological levels.…”

Section: Discussionmentioning

confidence: 78%

Toxicity of pyrethroid cypermethrin on the freshwater snail Chilina parchappii: Lethal and sublethal effects

Juan

Cortelezzi

Albornoz

et al. 2020

Ecotoxicology and Environmental Safety

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“…Mukhopadhyay et al [38] have found similar results, revealing a significant dose-dependent increase in DNA damage in the cells of brain ganglia and anterior midgut of Drosophila melanogaster exposed to low concentrations of Cyp (0.0004, 0.0008, 0.002, 0.2, and 0.5 ppm). In fish, Poletta et al [39] found a significant increase in DNA damage of epithelial gill cells of P. lineatus after in vivo acute exposure (96 h) to 0.15 and 0.3 μg/L Cyp compared to controls.…”

Section: Histological and Immunofluorescence Analysismentioning

confidence: 99%

Genotoxicity Induced by Cypermethrin in the Zebrafish Retina

Paravani¹,

Casco²

2018

Genotoxicity - A Predictable Risk to Our Actual World

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Cypermethrin (Cyp), is one of the most common contaminants in freshwater aquatic systems. We evaluated its possible genotoxic effect and oxidative stress in retinal cells of adult zebrafish exposed to 0.3 μg/L and 0.6 μg/L Cyp. Both the histological and immunofluorescence (IF) techniques showed the presence of apoptotic cells in the zebrafish retina after 9 days of treatment with 0.6 μg/L Cyp. Thus, histone γ-H2AX, a double-stranded DNA damage marker, was immunodetected in both the outer and inner nuclear layer after exposure to 0.6 μg/L Cyp for 12 days, while the anti-caspase-3 apoptotic antibody was detected in the outer nuclear layer. Compared with the morphological evidence, the damage index (DI) showed significant differences with 0.3 μg/L from day 9, while with 0.6 μg/L all the stages evaluated showed very significant differences. According to these results, it was verified that the activities of superoxide dismutase (SOD) and catalase (CAT) increased significantly after exposure to 0.6 μg/L Cyp. The same treatment caused a significant positive regulation of the mRNA levels of both genes. These results indicate that Cyp causes DNA damage and oxidative stress. This pyrethroid also has the potential to induce apoptosis in the cells of the retina.

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Comet assay in gill cells of Prochilodus lineatus exposed in vivo to cypermethrin

Cited by 25 publications

References 37 publications

Induction of DNA base damage and strand breaks in peripheral erythrocytes and the underlying mechanism in goldfish (Carassius auratus) exposed to monocrotophos

Induction of DNA base damage and strand breaks in peripheral erythrocytes and the underlying mechanism in goldfish (Carassius auratus) exposed to monocrotophos

Toxicity of pyrethroid cypermethrin on the freshwater snail Chilina parchappii: Lethal and sublethal effects

Genotoxicity Induced by Cypermethrin in the Zebrafish Retina

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