Comet and micronucleus assays do not correlate extent of DNA damage with clinical staging of oral cavity squamous cell carcinoma

Santos, Andreza Negreli; Oliveira, Rodrigo Juliano; Pessatto, Lucas Roberto; Souza, Albert Schiavetto; Antoniolli-Silva, Andréia Conceição Milan Brochado; Cunha-Laura, Andréa Luiza; Levenhagen, Maria Margarida Morena Domingos; Freitas, Carlos Alberto Ferreira de

doi:10.4322/ahns.2020.0013

Arch. Head Neck Surg.

2020

DOI: 10.4322/ahns.2020.0013

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Comet and micronucleus assays do not correlate extent of DNA damage with clinical staging of oral cavity squamous cell carcinoma

Andreza Negreli Santos¹,

Rodrigo Juliano Oliveira²,

Lucas Roberto Pessatto³

et al.

Abstract: Financial support: None. Conflicts of interest: No conflicts of interest declared concerning the publication of this article.

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2024

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Cited by 2 publications

References 26 publications

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Measurement of chromosomal instability and level of DNA damage in peripheral blood mononuclear cells of endometrial cancer patients

Marković,

Grujičić,

Živković Radojević

et al. 2024

Mutagenesis

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Endometrial cancer is one of the most common invasive gynecologic malignancies in developed countries. The aim of this study was to evaluate chromosomal instability and level of DNA damage in peripheral blood mononuclear cells (PBMCs) of newly diagnosed endometrial cancer patients in relation to health status (diagnosis), age, histological grade of cancer, residence, smoking, number of pregnancies, miscarriages, and abortions. The analyzed sample consisted of 60 individuals, 30 endometrial cancer patients with an average age of 64.37 ± 7.08, and 30 healthy control women average age of 60.23 ± 11.55. Chromosomal instability was evaluated by the cytokinesis-block micronucleus (CBMN) assay and the level of DNA damage by the single-cell gel electrophoresis (comet) assay in PBMCs. The average frequencies of micronuclei (MNi), nucleoplasmic bridges (NPBs) as well as nuclear buds (NBUDs) were significantly higher in cancer patients compared to controls (p < 0.0005). There was no difference in the nuclear division index (NDI) among the analyzed samples. The comet assay showed that the patients had a significantly increased genetic damage index (GDI) compared with controls (p < 0.0005). Using linear regression analysis, we found that health status (diagnosis) had the strongest influence on the MN frequency as well as GDI (p < 0.0005). Our results indicated that there is a high level of genetic damage in both the level of DNA and the level of chromosomes in the PBMCs of newly diagnosed patients with endometrial cancer, where the frequency and level of damage were significantly affected by health status, grade of cancer, residence, number of pregnancies, miscarriages, and abortions.

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Measurement of chromosomal instability and level of DNA damage in peripheral blood mononuclear cells of endometrial cancer patients

Marković,

Grujičić,

Živković Radojević

et al. 2024

Mutagenesis

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Evaluation of DNA Damage in Oral Squamous Cell Carcinoma by Comet Assay

Halder,

Sardar,

Mondal

2024

Journal of Primary Care Dentistry and Oral Health

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Background: The Comet assay is a technique used to detect DNA damage at the single-cell level. This study aimed to investigate the potential relationship between the histopathological grading of oral squamous cell carcinoma (OSCC) and genotoxicity (DNA damage). Methods: Blood samples were collected from both normal subjects and patients diagnosed with OSCC. Slides prepared for the Comet assay were analyzed using an electrofluorescent microscope with a calibrated eyepiece to measure the total image length and head diameter. DNA tail length was calculated by subtracting the head diameter from the total length. Results: In the evaluation of DNA damage in 26 OSCC patients compared to 10 controls, a significantly higher level of DNA damage was observed in OSCC patients (P < 0.001). When considering different histological grades, the mean DNA damage was found to be highest in poorly differentiated (29.9 μm), followed by moderately differentiated (25.60 μm), and lowest in well-differentiated (23.28 μm) cases. Although differences in the average DNA damage did not reach statistical significance among the histological grades (P > 0.05), all “t” values fell below the critical values at the 5% level, indicating no significant differences between the stages. Conclusions: The extent of DNA damage in OSCC was highest in poorly differentiated cases, followed by moderately differentiated and well-differentiated cases. These findings suggest a potential link between histopathological grading and DNA damage in OSCC.

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Comet and micronucleus assays do not correlate extent of DNA damage with clinical staging of oral cavity squamous cell carcinoma

Abstract: Financial support: None. Conflicts of interest: No conflicts of interest declared concerning the publication of this article.

Cited by 2 publications

References 26 publications

Measurement of chromosomal instability and level of DNA damage in peripheral blood mononuclear cells of endometrial cancer patients

Measurement of chromosomal instability and level of DNA damage in peripheral blood mononuclear cells of endometrial cancer patients

Evaluation of DNA Damage in Oral Squamous Cell Carcinoma by Comet Assay

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