Combining rare alleles and grouped pollen donors to assign paternity in pollen dispersal studies

Butcher, Chelsea L.; Rubin, Berish Y.; Anderson, Sylvia L.; Nandula, Vijay K.; Owen, Micheal D. K.; Gardner, Richard G.

doi:10.1002/aps3.11330

Cited by 2 publications

(4 citation statements)

References 49 publications

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“…Seeds were germinated and seedlings were grown in a greenhouse at the Louis Calder Center (Fordham University) field station in Armonk, New York, USA during the spring of 2015 ((Solanum lycopersicum -April/May; Amaranthus tuberculatus -May/June), following Butcher et al (2020b). Briefly, seeds were germinated in 53 × 28 × 5 cm (l × w × d) potting trays (Griffin Greenhouse Supplies, Tewksbury, Massachusetts, USA) containing Sungro Sunshine Soilmix 4 (Sungro Horticulture, Agawam, Massachusetts, USA) supplemented with Osmocote 14-14-14 fertilizer (Scotts Miracle-Gro, Marysville, Ohio, USA).…”

Section: Greenhouse Methodsmentioning

confidence: 99%

“…Molecular techniques used for paternity assignment followed Butcher et al (2020b), except that Amaranthus tuberculatus hypocotyl and cotyledon tissue was incubated with AP1 buffer and RNase A (Qiagen, Hilden, Germany) for 2 hr after disruption. In brief, we extracted DNA from maternal and paternal leaf tissue, as well as from seeds produced during the experiment.…”

Section: Paternity Assignmentmentioning

confidence: 99%

“…This typically requires a multi-marker approach of genotyping all potential pollen donors and offspring to assign paternity to a single pollen donor (i.e., paternity analysis) (Van Rossum et al, 2011). Alternatively, a rare allele approach coupled with grouped pollen donors can be used to determine paternity and measure effective pollen dispersal after genotyping a limited number of pollen donors and a representative number of offspring (Butcher et al, 2020b).…”

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confidence: 99%

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Pollen dispersal patterns differ among sites for a wind‐pollinated species and an insect‐pollinated species

Butcher

Rubin

Anderson

2020

American J of Botany

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Premise Pollen dispersal, the main component of overall plant gene flow, generally decreases with increasing distance from the pollen source, but the pattern of this relationship may differ among sites. Although site‐based differences in pollen dispersal may lead to over‐ or underestimation of gene flow, no studies have investigated pollen dispersal patterns among differing urban site types, despite the incongruent range of habitats in urban areas. Methods We used paternity assignment to assess pollen dispersal patterns in a wind‐pollinated species (waterhemp; Amaranthus tuberculatus) and in an insect‐pollinated species (tomato; Solanum lycopersicum) in experimental arrays at four disparate sites (two roof‐level sites, two ground‐level sites) in the New York (New York, USA) metropolitan area. Results The number of seeds or fruits, a proxy for the number of flowers pollinated, decreased with increasing distance from the pollen donors at all sites for both species. However, the mean number of Amaranthus tuberculatusseeds produced at a given distance differed two‐fold among sites, while the slope of the relationship between Solanum lycopersicumfruit production and distance differed by a factor of four among sites. Conclusions Pollen dispersal patterns may differ substantially among sites, both in the amount of pollen dispersed at a given distance and in the proportional decrease in pollen dispersal with increasing distance, and these effects may act independently. Accordingly, the capacity of plant species to adapt to climate change and other selection pressures may be different from predictions based on pollen dispersal patterns at a single location.

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Section: Greenhouse Methodsmentioning

confidence: 99%

Section: Paternity Assignmentmentioning

confidence: 99%

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confidence: 99%

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Pollen dispersal patterns differ among sites for a wind‐pollinated species and an insect‐pollinated species

Butcher

Rubin

Anderson

2020

American J of Botany

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“…Paternity was assigned using the rare allele approach, as described in Butcher et al (2020b). In brief, all A. tuberculatus pollen receptor and donor plants, and a subset of seeds, were genotyped at markers for the external transcribed spacer region (ETS) and putative DEAD box ATP-dependent RNA helicase gene (PutDead).…”

Section: Paternity Assignmentmentioning

confidence: 99%

Long-Distance Pollen Dispersal in Urban Green Roof and Ground-Level Habitats

2022

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Long-distance pollen dispersal is critical for gene flow in plant populations, yet pollen dispersal patterns in urban habitats such as green roofs have not been extensively studied. Pollen dispersal patterns typically are assessed either by fitting non-linear models to the relationship between the degree of pollen dispersal and distance to the pollen source (i.e., curve fitting), or by fitting probability density functions (PDFs) to pollen dispersal probability histograms (i.e., PDF fitting). Studies using curve fitting typically report exponential decay patterns in pollen dispersal. However, PDF fitting typically produces more fat-tailed distributions, suggesting the exponential decay may not be the best fitting model. Because the two approaches may yield conflicting results, we used both approaches to examine pollen dispersal patterns in the wind-pollinated Amaranthus tuberculatus and the insect-pollinated Solanum lycopersicum at two green roof and two ground-level sites in the New York (NY, United States) metropolitan area. For the curve fitting analyses, the exponential decay and inverse power curves provided good fits to pollen dispersal patterns across both green roof and ground-level sites for both species. Similar patterns were observed with the PDF fitting analyses, where the exponential or inverse Gaussian were the top PDF at most sites for both species. While the curve fitting results are consistent with other studies, the results differ from most studies using PDF fitting, where long-distance pollen dispersal is more common than we observed. These results highlight the need for further research to compare curve and PDF fitting for predicting pollen dispersal patterns. And, critically, while long-distance pollen dispersal may be an important component of overall pollen dispersal for A. tuberculatus and S. lycopersicum in both urban green roof and ground-level sites, our results suggest it potentially may occur to a lesser extent compared with plants in less-urban areas.

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Combining rare alleles and grouped pollen donors to assign paternity in pollen dispersal studies

Cited by 2 publications

References 49 publications

Pollen dispersal patterns differ among sites for a wind‐pollinated species and an insect‐pollinated species

Pollen dispersal patterns differ among sites for a wind‐pollinated species and an insect‐pollinated species

Long-Distance Pollen Dispersal in Urban Green Roof and Ground-Level Habitats

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