Combining Qdot Nanotechnology and DNA Nanotechnology for Sensitive Single‐Cell Imaging

Zhou, Wen; Han, Yan; Beliveau, Brian J.; Gao, Xiaohu

doi:10.1002/adma.201908410

Cited by 25 publications

(17 citation statements)

References 41 publications

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“…The labeling of target antigens was reversible and could be utilized for at least ten rounds of relabeling without affecting the sample antigenicity. [ 94 ]…”

Section: Nps As Mimics Of Fluorescent Proteinsmentioning

confidence: 99%

“…The labeling of target antigens was reversible and could be utilized for at least ten rounds of relabeling without affecting the sample antigenicity. [94] QDs with fluorescence emission in the first NIR window (NIR-I, 750-900 nm) have been widely exploited for in vivo fluorescence imaging. Moreover, a great improvement in imaging quality can be obtained through application of QDs that emit in the second NIR window (NIR-II, 1000-1700 nm), which is characterized by deep issue penetration and high spatial resolution.…”

Section: Qdsmentioning

confidence: 99%

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Protein‐Mimicking Nanoparticles in Biosystems

Fan

2022

Advanced Materials

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Up to now, most protein-mimicking NPs can be classified into one of the four categories: 1) enzyme-like catalytic activities have been identified among a wide variety of nanomaterials including noble-metal-, metal-oxide-, carbon-, and metal-organic framework (MOF)-based nanozymes. Similar to engineered enzymes, the catalytic properties of nanozymes can be finetuned by the change of composition and surface modification. In addition, dual-or multiple-enzyme mimetic activities can be integrated into one type of NPs. [3,4] 2) Fluorescent NPs such as quantum dots (QDs), upconversion NPs (UCNPs), aggregation-induced emission (AIE) NPs, and semiconducting polymer NPs (SPNPs)/polymer dots (Pdots), can serve as attractive alternatives or even better replacement of fluorescent proteins, bringing broader benefits for bioimaging and light-activated therapy. NPs of this category possess high fluorescence intensity, tunable fluorescence wavelengths, and high photostability. These unique optical properties make them ideal fluorophores for long-term tracking and simultaneous monitoring of multiple biological species. [5] Moreover, some types of fluorescent NPs can be tuned to emit in the near-infrared (NIR) window, providing great advantages for deep-tissue imaging. [6] 3) More and more studies reveal that certain types of NPs show high affinity binding to specific proteins or DNA sequences. Such interaction leads to the utilization of NPs for protein or DNA recognition, and may actively alter cellular signaling and communication when introduced into living systems. [7,8] Alternatively, the DNA binding capacity can be used for the docking of DNA strands for the assembly of supramolecular structures. [9] 4) The bottom-up construction of NPs has created a diversity of nanostructures with close similarities in both morphology and function to natural protein scaffolds. NPs based on DNA strands, polymers, or carbon nanotubes (CNTs) can form membrane-embedded hollow structures (nanopores) analogous to biological ion channels. Extracellular or intracellular scaffold-mimicking NPs have been designed to mediate cell-cell contact, communication, and signaling cascade. [10,11] Besides, curved nanostructures can mimic membrane sculpting proteins to mediate membrane extrusion or invagination. [12,13] Compared with natural proteins, the abovementioned categories of protein-mimicking NPs are cost-efficient, physically, and chemically stable, and can be constructed with more versatility for translating into a broad range of applications.Proteins are essential elements for almost all life activities. The emergence of nanotechnology offers innovative strategies to create a diversity of nanoparticles (NPs) with intrinsic capacities of mimicking the functions of proteins. These artificial mimics are produced in a cost-efficient and controllable manner, with their protein-mimicking performances comparable or superior to those of natural proteins. Moreover, they can be endowed with additional functionalities that are absent in natural proteins, such ...

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“…The labeling of target antigens was reversible and could be utilized for at least ten rounds of relabeling without affecting the sample antigenicity. [ 94 ]…”

Section: Nps As Mimics Of Fluorescent Proteinsmentioning

confidence: 99%

Section: Qdsmentioning

confidence: 99%

Protein‐Mimicking Nanoparticles in Biosystems

Fan

2022

Advanced Materials

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“…Another approach uses DNA-barcoded antibodies that are visualized by cyclic addition and removal of fluorescently labeled DNA probes. Techniques based on this principle include exchangepoints accumulation in nanoscale topography (PAINT) [22], DNA exchange imaging (DEI) [23], immunostaining with signal amplification by exchange reaction (immuno-SABER) [24], quantumdot SABER [25], barcoded-antibody based cyclic immunofluorescence (cyCIF) [26], and CO-Detection by indEXing (CODEX) [27,28]. These DNA-based systems have the advantages of a single staining procedure, fast run times, and comparably simple chemistries.…”

Section: Introductionmentioning

confidence: 99%

Highly multiplexed tissue imaging using repeated oligonucleotide exchange reaction

et al. 2021

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Multiparameter tissue imaging enables analysis of cell-cell interactions in situ, the cellular basis for tissue structure, and novel cell types that are spatially restricted, giving clues to biological mechanisms behind tissue homeostasis and disease. Here, we streamlined and simplified the multiplexed imaging method CO-Detection by indEXing (CODEX) by validating 58 unique oligonucleotide barcodes that can be conjugated to antibodies. We showed that barcoded antibodies retained their specificity for staining cognate targets in human tissue. Antibodies were visualized one at a time by adding a fluorescently labeled oligonucleotide complementary to oligonucleotide barcode, imaging, stripping, and repeating this cycle. With this we developed a panel of 46 antibodies that was used to stain five human lymphoid tissues: three tonsils, a spleen, and a LN. To analyze the data produced, an image processing and analysis pipeline was developed that enabled single-cell analysis on the data, including unsupervised clustering, that revealed 31 cell types across all tissues. We compared cell-type compositions within and directly surrounding follicles from the different lymphoid organs and evaluated cell-cell density correlations. This sequential oligonucleotide exchange technique enables a facile imaging of tissues that leverages pre-existing imaging infrastructure to decrease the barriers to broad use of multiplexed imaging.

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“…Quantum Dots (QDs), due to their unique optical-electronic properties compared to traditional labeling reagents (organic-and protein-based fluorophores), has been attracting wide interest in bioimaging and biosensing, and a significant progress has been made thanks to the sophisticated surface coating technology developed especially for CdSe@ZnS core-shell QDs [ [1] , [2] , [3] , [4] , [5] , [6] , [7] , [8] , [9] , [10] , [11] , [12] ]. However, to the best of our knowledge, most of previous reported QDs-based fluorescence methods for bioassay always suffer from low sensitivity compared to that of clinic ones, such as chemical luminescence immunoassay (CLIA) [ 13 ], digital enzyme-linked immunosorbent assay (digital ELISA) [ 7 ], and time-resolved fluorescence immunoassay (TRFIA) [ 14 ], among which the sensitivity for protein detection could reach subfemtomolar concentration.…”

Section: Introductionmentioning

confidence: 99%