Combining pollination ecology and fine-scale spatial genetic structure analysis to unravel the reproductive strategy of an insular threatened orchid

Gigant, Rodolphe; Bruyn, Alexandre De; M'sa, Tachirifa; Viscardi, G. P.; Gigord, Luc D. B.; Gauvin‐Bialecki, Anne; Pailler, Thierry; Humeau, Laurence; Grisoni, Michel; Besse, Pascale

doi:10.1016/j.sajb.2016.02.205

Cited by 15 publications

(45 citation statements)

References 73 publications

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“…This is the case for example for V. humblotii Rchb. f., endangered (EN) in Mayotte [13]. Vanilla wild relatives therefore deserve special attention.…”

Section: Introductionmentioning

confidence: 99%

“…Within our current efforts to determine the reproductive biology and genetic diversity in vanilla CWR, which led us so far to study V. roscheri Rchb. f. in South Africa [20] and V. humblotii in Mayotte [13,21], we focused on wild populations of V. mexicana occurring in the island of Guadeloupe (French west indies) to unravel its mating system. The vast majority of Vanilla species displays a mixed reproductive mode [1,4] with both asexual and sexual reproduction.…”

Section: Introductionmentioning

confidence: 99%

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Microsatellite Markers Confirm Self‐Pollination and Autogamy in Wild Populations of Vanilla mexicana Mill. (syn. V. inodora) (Orchidaceae) in the Island of Guadeloupe

Gigant¹,

Rakotomanga²,

Goulié³

et al. 2016

Microsatellite Markers

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The study aimed at evaluating the mating system of Vanilla mexicana (Orchidaceae) in natural populations in the island of Guadeloupe. A total of 132 V. mexicana samples were collected from 12 sites in Guadeloupe (Basse-Terre). Five other samples coming from Martinique and Mexico completed our analyses. Reproductive biology experiments excluding pollinators with bagged flowers revealed 53.9% fruit set, a value identical to the natural fruit set measured in the populations. These results suggested that V. mexicana, unlike most Vanilla species, was reproducing by self-pollination and autogamy. Due to lack of specific DNA markers for V. mexicana, microsatellite markers, previously developed in other Vanilla species, were used for the genetic analyses. Only 6 out of the 33 markers tested were transferable and polymorphic in V. mexicana. A panel of 51 V. mexicana samples genotyped with 3 polymorphic loci was finally retained for Guadeloupe population genetic analyses. A heterozygote deficiency was detected, and the selfing rate was estimated to 74%. These results confirmed the reproductive biology results as self-pollination and autogamy were the most likely explanation for this deficit. Results were compared to those from allogamous wild Vanilla species and discussed in the light of suggested existence of a pollinator for V. mexicana in other areas (Mexico).

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“…This is the case for example for V. humblotii Rchb. f., endangered (EN) in Mayotte [13]. Vanilla wild relatives therefore deserve special attention.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microsatellite Markers Confirm Self‐Pollination and Autogamy in Wild Populations of Vanilla mexicana Mill. (syn. V. inodora) (Orchidaceae) in the Island of Guadeloupe

Gigant¹,

Rakotomanga²,

Goulié³

et al. 2016

Microsatellite Markers

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“…Bromeliaceae and Cactaceae); (x) encourage a broad network of people (local population and visitors) to gather and share information on the location of orchid species with the managers of the APA Abaeté, Parque das Dunas and Parque do Abaeté; and (xi) species management, including artificial pollination, asexual reproduction, and transportation of species to other locations of APA Abaeté (e.g. Vanilla palmarum is autogamous and has high pollination rates (Householder et al 2010, Soto Arenas & Cribb 2010; seeds can be dispersed on E. guineensis).…”

Section: Discussionmentioning

confidence: 99%

Mapping Threats to the Orchid Populations in an Environmental Protection Area in Bahia, Northeast Brazil

2019

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The Área de Proteção Ambiental das Lagoas e Dunas do Abaeté (APA Abaeté) is an environmentally protected restinga fragment located in an urban area in the municipality of Salvador, the third most populous municipality in Brazil. We presented an updated list of Orchidaceae in APA Abaeté, including data on population size, aiming to support effective conservation strategies for orchids in Atlantic Forest remnants in the state. We carried out intensive fieldwork during October 2014 and February 2018, and analyzed several collections from Brazilian herbaria. Photographs, maps, short taxonomic notes and ecological information of the species are provided. We divided APA Abaeté into 16 plots (each with about 1.9 km 2) to facilitate the discussion regarding local conservation actions. We found 15 genera and 19 species, including highly ornamental orchids (e.g. Brassavola tuberculata and Encyclia dichroma). Most species are terricolous and occur in the restinga forest formation (68%). We recognized six species as new occurrences for APA Abaeté: Catasetum roseoalbum, Eltroplectris calcarata, Habenaria schenckii, Liparis nervosa, Pelexia viridis and Prescottia leptostachya. Epistephium williamsii, Galeandra montana, H. schenckii, P. viridis and Vanilla palmarum deserve immediate conservation actions in order to avoid local extinctions. The mentioned species form small populations (less than 50 individuals) and/or are restricted to up to three plots. Deforestation, occasional changes in habitats, including illegal collection of plants, urban growth of the neighborhoods, and the possible expansion of the international airport in Salvador may drastically reduce the population sizes of the orchids present. Cyrtopodium parviflorum and Koellensteinia florida may be extinct locally; they were last collected last century and recent attempts to find them in the area were unsuccessful. Conservation efforts must include the management of species, environmental educational actions and the permanent control and surveillance of the area.

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“…(Trapnell et al 2004) and Vanilla humblotii. (Gigant et al 2016). Of these studies, only Trapnell et al (2004) examined fine-scale structure with high detail (i.e., sampling of all individuals within a tree host) showing significant patterns of relatedness at distances of less than 45 cm, likely due to a combination of vegetative reproduction and sexually derived offspring resulting from localized seed dispersal.…”

mentioning

confidence: 99%

“…While it is known that genetic diversity determined by microsatellite analysis are highly influenced by the type of repeat present in the markers (Merritt et al 2015) and that cross-species comparisons are more difficult, we can see that L. speciosa maintains population genetic diversity values similar to those found in a variety of other Orchidaceae ranging from the insular epiphytic Vanilla humblotii Rchb. f (H o = 0.450) (Gigant et al 2016), to the rare Cymbidium tortisepalum Fukuy. (H o = 0.619) (Zhao et al 2017) and the recently reduced in range grassland species Platanthera praeclara Sheviak & M. L. Bowles (H o = 0.566 -0.664) (Ross et al 2013).…”

mentioning

confidence: 99%

Maintenance of high levels of genetic diversity and significant fine-scale genetic structure among age classes in single-tree populations of the endangered epiphytic orchid Laelia speciosa (Orchidaceae) in central Mexico

2019

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Background: Epiphytic plants exist within the complex three-dimensional habitat of the tree canopy. This can have a profound effect on patterns of both genetic diversity and structure and may be important for conservation of epiphytic species. Objective: To evaluate how the epiphytic habit affects fine-scale three-dimensional genetic structure in single canopy tree populations. Studied species: Laelia speciosa, a threatened endemic Mexican epiphytic orchid. Study site and dates: Field sampling occurred during Spring 2006 in the municipality of Caurio de Guadalupe, Michoacán.Methods: All individuals occurring in two trees of Q. deserticola (178 in Tree 1 and 276 in Tree 2) were mapped, assigned to four discrete age classes, and genotyped at six microsatellite loci to understand patterns of genetic diversity and structure. Results: High levels of genetic diversity (polymorphic loci = 100 %, Ho = 0.398 – 0.506) occurred across size classes and trees but were not significantly different between the two trees. Significant fine-scale genetic structure was found in the Juvenile (plants 2-10 years old) and Adults I (11-20 years old) age classes in distances up to 15 cm. This significant structure was larger (up to 25 cm) when all individuals were treated as a single age class.Conclusion: The distribution and structure of genetic diversity is attributable to the epiphytic habit, particularly localized seed dispersal, limited selfing, and selection of adaptive traits. Conservation implications related to the sampling for in vitro propagation are given in light of the metapopulation genetic variability maintained within single-tree populations.

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Combining pollination ecology and fine-scale spatial genetic structure analysis to unravel the reproductive strategy of an insular threatened orchid

Cited by 15 publications

References 73 publications

Microsatellite Markers Confirm Self‐Pollination and Autogamy in Wild Populations of Vanilla mexicana Mill. (syn. V. inodora) (Orchidaceae) in the Island of Guadeloupe

Microsatellite Markers Confirm Self‐Pollination and Autogamy in Wild Populations of Vanilla mexicana Mill. (syn. V. inodora) (Orchidaceae) in the Island of Guadeloupe

Mapping Threats to the Orchid Populations in an Environmental Protection Area in Bahia, Northeast Brazil

Maintenance of high levels of genetic diversity and significant fine-scale genetic structure among age classes in single-tree populations of the endangered epiphytic orchid Laelia speciosa (Orchidaceae) in central Mexico

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