Combining dielectrophoresis and concentration polarization-based preconcentration to enhance bead-based immunoassay sensitivity

Park, Sinwook; Yossifon, Gilad

doi:10.1039/c9nr02506e

Cited by 22 publications

(29 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, previous approaches employing DEP force to immobilize beads and expose them to reagents relied on the use of planar electrodes to generate electric fields [ 20 ]. This approach relies on a simple fabrication process but leads to the creation of high electrokinetic forces only in close proximity to the chip’s surface that features the electrodes.…”

Section: Resultsmentioning

confidence: 99%

“…The height of the channel was then no longer limited and could be substantially increased, to 50 µm in this device and possibly larger, as only the microfabrication process dictates the extension of the microchannel height. Increasing the height of the channel helped us increase the reagent flow rate in the microfluidic chamber by a factor of 10 or more compared to similar detection platforms [ 18 , 20 ], improving the collection of analytes that may be only present at low concentration in serum.…”

Section: Resultsmentioning

confidence: 99%

“…It has been successfully employed to sort particles according to their size, shape, and dielectric properties [ 14 , 15 ]. It also proved to be valuable in the diagnostics field through enrichment of targeted protein [ 16 ], stream focusing in flow cytometry [ 17 ], and spatial confinement of decorated beads [ 18 , 19 , 20 ]. Iswardy et al [ 18 ] implemented a DEP-based biosensing platform for the diagnosis of Dengue virus.…”

Section: Introductionmentioning

confidence: 99%

“…Decorated beads are maintained against the flow with DEP and exposed to the sample of interest. A similar technique was proposed by Park et al [ 20 ], combining the manipulation of beads with DEP forces with a polarization-based preconcentration approach to increase the analyte concentration in the vicinity of beads. However, this solution imposes limitations on the flow rate and, thus, on the amount of analyte accessible for detection.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Rapid Multianalyte Microfluidic Homogeneous Immunoassay on Electrokinetically Driven Beads

et al. 2020

View full text Add to dashboard Cite

The simplicity of homogeneous immunoassays makes them suitable for diagnostics of acute conditions. Indeed, the absence of washing steps reduces the binding reaction duration and favors a rapid and compact device, a critical asset for patients experiencing life-threatening diseases. In order to maximize analytical performance, standard systems employed in clinical laboratories rely largely on the use of high surface-to-volume ratio suspended moieties, such as microbeads, which provide at the same time a fast and efficient collection of analytes from the sample and controlled aggregation of collected material for improved readout. Here, we introduce an integrated microfluidic system that can perform analyte detection on antibody-decorated beads and their accumulation in confined regions within 15 min. We employed the system to the concomitant analysis of clinical concentrations of Neutrophil Gelatinase-Associated Lipocalin (NGAL) and Cystatin C in serum, two acute kidney injury (AKI) biomarkers. To this end, high-aspect-ratio, three-dimensional electrodes were integrated within a microfluidic channel to impart a controlled trajectory to antibody-decorated microbeads through the application of dielectrophoretic (DEP) forces. Beads were efficiently retained against the fluid flow of reagents, granting an efficient on-chip analyte-to-bead binding. Electrokinetic forces specific to the beads’ size were generated in the same channel, leading differently decorated beads to different readout regions of the chip. Therefore, this microfluidic multianalyte immunoassay was demonstrated as a powerful tool for the rapid detection of acute life-threatening conditions.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Rapid Multianalyte Microfluidic Homogeneous Immunoassay on Electrokinetically Driven Beads

et al. 2020

View full text Add to dashboard Cite

show abstract

“…To address real-world needs for manipulation and fabrication of micro/nanomaterials, a number of micro-/nano-scaled methods have been presented. Typical examples include microfluidic [14,15], acoustic [16,17], electrokinetics [18,19], magnetic [20,21], optical [22,23], thermal [24,25], and atomic force microscope [26,27] approaches. An emerging topic on the manipulation and fabrication of micro/nanoparticles is about how to develop a novel mechanism that can complement what a single technique can offer.…”

Section: Introductionmentioning

confidence: 99%

A Review on Optoelectrokinetics-Based Manipulation and Fabrication of Micro/Nanomaterials

et al. 2020

View full text Add to dashboard Cite

Optoelectrokinetics (OEK), a fusion of optics, electrokinetics, and microfluidics, has been demonstrated to offer a series of extraordinary advantages in the manipulation and fabrication of micro/nanomaterials, such as requiring no mask, programmability, flexibility, and rapidness. In this paper, we summarize a variety of differently structured OEK chips, followed by a discussion on how they are fabricated and the ways in which they work. We also review how three differently sized polystyrene beads can be separated simultaneously, how a variety of nanoparticles can be assembled, and how micro/nanomaterials can be fabricated into functional devices. Another focus of our paper is on mask-free fabrication and assembly of hydrogel-based micro/nanostructures and its possible applications in biological fields. We provide a summary of the current challenges facing the OEK technique and its future prospects at the end of this paper.

show abstract

Biocompatible Direct Deposition of Functionalized Nanoparticles Using Shrinking Surface Plasmonic Bubble

Moon

Zhang

Huang

et al. 2020

Adv Materials Inter

View full text Add to dashboard Cite

Functionalized nanoparticles (NPs) are the foundation of diverse applications. Especially, in many biosensing applications, concentrating suspended NPs onto a surface without deteriorating their biofunction is usually an inevitable step to improve detection limit, which remains to be a great challenge. In this work, we demonstrate biocompatible deposition of functionalized NPs to optically transparent surfaces using shrinking bubbles. Leveraging the shrinking phase of bubble mitigates the biomolecule degradation problems encountered in traditional photothermal deposition techniques. The deposited NPs are closely packed, and the functional molecules are able to survive the process as verified by their strong fluorescence signals. Using high-speed videography, we reveal that the contracting contact line of the shrinking bubble forces the NPs captured by the contact line to a highly concentrated island. Such shrinking surface bubble deposition (SSBD) is low temperature in nature as no heat is added during the process. Using a hairpin DNA-functionalized gold NP suspension as a model system, SSBD is shown to enable much stronger fluorescence signal compared to the optical-pressure deposition and the conventional thermal bubble contact line deposition. The demonstrated SSBD technique capable of directly depositing functionalized NPs may significantly simplify biosensor fabrication and thus benefit a wide range of relevant applications.

show abstract

Combining dielectrophoresis and concentration polarization-based preconcentration to enhance bead-based immunoassay sensitivity

Abstract: Ionic concentration-polarization (CP)-based simultaneous preconcentration of functionalized nanoparticles and target biomolecules followed by dynamic trapping of the beads using dielectrophoresis (DEP) for binding signal assessment.

Cited by 22 publications

References 47 publications

Rapid Multianalyte Microfluidic Homogeneous Immunoassay on Electrokinetically Driven Beads

Rapid Multianalyte Microfluidic Homogeneous Immunoassay on Electrokinetically Driven Beads

A Review on Optoelectrokinetics-Based Manipulation and Fabrication of Micro/Nanomaterials

Biocompatible Direct Deposition of Functionalized Nanoparticles Using Shrinking Surface Plasmonic Bubble

Contact Info

Product

Resources

About